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Development of a flow chamber system for the reproducible in vitro analysis of biofilm formation on implant materials

Since the introduction of modern dental implants in the 1980s, the number of inserted implants has steadily increased. Implant systems have become more sophisticated and have enormously enhanced patients’ quality of life. Although there has been tremendous development in implant materials and clinic...

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Autores principales: Rath, Henryke, Stumpp, Sascha Nico, Stiesch, Meike
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5302373/
https://www.ncbi.nlm.nih.gov/pubmed/28187188
http://dx.doi.org/10.1371/journal.pone.0172095
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author Rath, Henryke
Stumpp, Sascha Nico
Stiesch, Meike
author_facet Rath, Henryke
Stumpp, Sascha Nico
Stiesch, Meike
author_sort Rath, Henryke
collection PubMed
description Since the introduction of modern dental implants in the 1980s, the number of inserted implants has steadily increased. Implant systems have become more sophisticated and have enormously enhanced patients’ quality of life. Although there has been tremendous development in implant materials and clinical methods, bacterial infections are still one of the major causes of implant failure. These infections involve the formation of sessile microbial communities, called biofilms. Biofilms possess unique physical and biochemical properties and are hard to treat conventionally. There is a great demand for innovative methods to functionalize surfaces antibacterially, which could be used as the basis of new implant technologies. Present, there are few test systems to evaluate bacterial growth on these surfaces under physiological flow conditions. We developed a flow chamber model optimized for the assessment of dental implant materials. As a result it could be shown that biofilms of the five important oral bacteria Streptococcus gordonii, Streptococcus oralis, Streptococcus salivarius, Porphyromonas gingivalis, and Aggregatibacter actinomycetemcomitans, can be reproducibly formed on the surface of titanium, a frequent implant material. This system can be run automatically in combination with an appropriate microscopic device and is a promising approach for testing the antibacterial effect of innovative dental materials.
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spelling pubmed-53023732017-02-28 Development of a flow chamber system for the reproducible in vitro analysis of biofilm formation on implant materials Rath, Henryke Stumpp, Sascha Nico Stiesch, Meike PLoS One Research Article Since the introduction of modern dental implants in the 1980s, the number of inserted implants has steadily increased. Implant systems have become more sophisticated and have enormously enhanced patients’ quality of life. Although there has been tremendous development in implant materials and clinical methods, bacterial infections are still one of the major causes of implant failure. These infections involve the formation of sessile microbial communities, called biofilms. Biofilms possess unique physical and biochemical properties and are hard to treat conventionally. There is a great demand for innovative methods to functionalize surfaces antibacterially, which could be used as the basis of new implant technologies. Present, there are few test systems to evaluate bacterial growth on these surfaces under physiological flow conditions. We developed a flow chamber model optimized for the assessment of dental implant materials. As a result it could be shown that biofilms of the five important oral bacteria Streptococcus gordonii, Streptococcus oralis, Streptococcus salivarius, Porphyromonas gingivalis, and Aggregatibacter actinomycetemcomitans, can be reproducibly formed on the surface of titanium, a frequent implant material. This system can be run automatically in combination with an appropriate microscopic device and is a promising approach for testing the antibacterial effect of innovative dental materials. Public Library of Science 2017-02-10 /pmc/articles/PMC5302373/ /pubmed/28187188 http://dx.doi.org/10.1371/journal.pone.0172095 Text en © 2017 Rath et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Rath, Henryke
Stumpp, Sascha Nico
Stiesch, Meike
Development of a flow chamber system for the reproducible in vitro analysis of biofilm formation on implant materials
title Development of a flow chamber system for the reproducible in vitro analysis of biofilm formation on implant materials
title_full Development of a flow chamber system for the reproducible in vitro analysis of biofilm formation on implant materials
title_fullStr Development of a flow chamber system for the reproducible in vitro analysis of biofilm formation on implant materials
title_full_unstemmed Development of a flow chamber system for the reproducible in vitro analysis of biofilm formation on implant materials
title_short Development of a flow chamber system for the reproducible in vitro analysis of biofilm formation on implant materials
title_sort development of a flow chamber system for the reproducible in vitro analysis of biofilm formation on implant materials
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5302373/
https://www.ncbi.nlm.nih.gov/pubmed/28187188
http://dx.doi.org/10.1371/journal.pone.0172095
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