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Synergistic Antibacterial Effects of Nanoparticles Encapsulated with Scutellaria baicalensis and Pure Chlorhexidine on Oral Bacterial Biofilms

Scutellaria baicalensis (SB) is a traditional Chinese medicine for treating infectious and inflammatory diseases. Our recent study shows potent antibacterial effects of nanoparticle-encapsulated chlorhexidine (Nano-CHX). Herein, we explored the synergistic effects of the nanoparticle-encapsulated SB...

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Autores principales: Leung, Ken Cham-Fai, Seneviratne, Chaminda Jayampath, Li, Xuan, Leung, Ping Chung, Lau, Clara Bik San, Wong, Chi-Hin, Pang, Ka Yan, Wong, Chun Wai, Wat, Elaine, Jin, Lijian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5302556/
https://www.ncbi.nlm.nih.gov/pubmed/28335189
http://dx.doi.org/10.3390/nano6040061
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author Leung, Ken Cham-Fai
Seneviratne, Chaminda Jayampath
Li, Xuan
Leung, Ping Chung
Lau, Clara Bik San
Wong, Chi-Hin
Pang, Ka Yan
Wong, Chun Wai
Wat, Elaine
Jin, Lijian
author_facet Leung, Ken Cham-Fai
Seneviratne, Chaminda Jayampath
Li, Xuan
Leung, Ping Chung
Lau, Clara Bik San
Wong, Chi-Hin
Pang, Ka Yan
Wong, Chun Wai
Wat, Elaine
Jin, Lijian
author_sort Leung, Ken Cham-Fai
collection PubMed
description Scutellaria baicalensis (SB) is a traditional Chinese medicine for treating infectious and inflammatory diseases. Our recent study shows potent antibacterial effects of nanoparticle-encapsulated chlorhexidine (Nano-CHX). Herein, we explored the synergistic effects of the nanoparticle-encapsulated SB (Nano-SB) and Nano-CHX on oral bacterial biofilms. Loading efficiency of Nano-SB was determined by thermogravimetric analysis, and its releasing profile was assessed by high-performance liquid chromatographyusing baicalin (a flavonoid compound of SB) as the marker. The mucosal diffusion assay on Nano-SB was undertaken in a porcine model. The antibacterial effects of the mixed nanoparticles (Nano-MIX) of Nano-SB and Nano-CHX at 9:1 (w/w) ratio were analyzed in both planktonic and biofilm modes of representative oral bacteria. The Nano-MIX was effective on the mono-species biofilms of Streptococcus (S.) mutans, S. sobrinus, Fusobacterium (F.) nucleatum, and Aggregatibacter (A.) actinomycetemcomitans (MIC 50 μg/mL) at 24 h, and exhibited an enhanced effect against the multi-species biofilms such as S. mutans, F. nucleatum, A. actinomycetemcomitans, and Porphyromonas (P.) gingivalis (MIC 12.5 μg/mL) at 24 h that was supported by the findings of both scanning electron microscopy (SEM) and confocal scanning laser microscopy (CLSM). This study shows enhanced synergistic antibacterial effects of the Nano-MIX on common oral bacterial biofilms, which could be potentially developed as a novel antimicrobial agent for clinical oral/periodontal care.
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spelling pubmed-53025562017-03-21 Synergistic Antibacterial Effects of Nanoparticles Encapsulated with Scutellaria baicalensis and Pure Chlorhexidine on Oral Bacterial Biofilms Leung, Ken Cham-Fai Seneviratne, Chaminda Jayampath Li, Xuan Leung, Ping Chung Lau, Clara Bik San Wong, Chi-Hin Pang, Ka Yan Wong, Chun Wai Wat, Elaine Jin, Lijian Nanomaterials (Basel) Article Scutellaria baicalensis (SB) is a traditional Chinese medicine for treating infectious and inflammatory diseases. Our recent study shows potent antibacterial effects of nanoparticle-encapsulated chlorhexidine (Nano-CHX). Herein, we explored the synergistic effects of the nanoparticle-encapsulated SB (Nano-SB) and Nano-CHX on oral bacterial biofilms. Loading efficiency of Nano-SB was determined by thermogravimetric analysis, and its releasing profile was assessed by high-performance liquid chromatographyusing baicalin (a flavonoid compound of SB) as the marker. The mucosal diffusion assay on Nano-SB was undertaken in a porcine model. The antibacterial effects of the mixed nanoparticles (Nano-MIX) of Nano-SB and Nano-CHX at 9:1 (w/w) ratio were analyzed in both planktonic and biofilm modes of representative oral bacteria. The Nano-MIX was effective on the mono-species biofilms of Streptococcus (S.) mutans, S. sobrinus, Fusobacterium (F.) nucleatum, and Aggregatibacter (A.) actinomycetemcomitans (MIC 50 μg/mL) at 24 h, and exhibited an enhanced effect against the multi-species biofilms such as S. mutans, F. nucleatum, A. actinomycetemcomitans, and Porphyromonas (P.) gingivalis (MIC 12.5 μg/mL) at 24 h that was supported by the findings of both scanning electron microscopy (SEM) and confocal scanning laser microscopy (CLSM). This study shows enhanced synergistic antibacterial effects of the Nano-MIX on common oral bacterial biofilms, which could be potentially developed as a novel antimicrobial agent for clinical oral/periodontal care. MDPI 2016-04-07 /pmc/articles/PMC5302556/ /pubmed/28335189 http://dx.doi.org/10.3390/nano6040061 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Leung, Ken Cham-Fai
Seneviratne, Chaminda Jayampath
Li, Xuan
Leung, Ping Chung
Lau, Clara Bik San
Wong, Chi-Hin
Pang, Ka Yan
Wong, Chun Wai
Wat, Elaine
Jin, Lijian
Synergistic Antibacterial Effects of Nanoparticles Encapsulated with Scutellaria baicalensis and Pure Chlorhexidine on Oral Bacterial Biofilms
title Synergistic Antibacterial Effects of Nanoparticles Encapsulated with Scutellaria baicalensis and Pure Chlorhexidine on Oral Bacterial Biofilms
title_full Synergistic Antibacterial Effects of Nanoparticles Encapsulated with Scutellaria baicalensis and Pure Chlorhexidine on Oral Bacterial Biofilms
title_fullStr Synergistic Antibacterial Effects of Nanoparticles Encapsulated with Scutellaria baicalensis and Pure Chlorhexidine on Oral Bacterial Biofilms
title_full_unstemmed Synergistic Antibacterial Effects of Nanoparticles Encapsulated with Scutellaria baicalensis and Pure Chlorhexidine on Oral Bacterial Biofilms
title_short Synergistic Antibacterial Effects of Nanoparticles Encapsulated with Scutellaria baicalensis and Pure Chlorhexidine on Oral Bacterial Biofilms
title_sort synergistic antibacterial effects of nanoparticles encapsulated with scutellaria baicalensis and pure chlorhexidine on oral bacterial biofilms
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5302556/
https://www.ncbi.nlm.nih.gov/pubmed/28335189
http://dx.doi.org/10.3390/nano6040061
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