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The Influence of Modified Silica Nanomaterials on Adult Stem Cell Culture

The preparation of tailored nanomaterials able to support cell growth and viability is mandatory for tissue engineering applications. In the present work, silica nanoparticles were prepared by a sol-gel procedure and were then functionalized by condensation of amino groups and by adsorption of silve...

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Autores principales: Tarpani, Luigi, Morena, Francesco, Gambucci, Marta, Zampini, Giulia, Massaro, Giuseppina, Argentati, Chiara, Emiliani, Carla, Martino, Sabata, Latterini, Loredana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5302627/
https://www.ncbi.nlm.nih.gov/pubmed/28335232
http://dx.doi.org/10.3390/nano6060104
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author Tarpani, Luigi
Morena, Francesco
Gambucci, Marta
Zampini, Giulia
Massaro, Giuseppina
Argentati, Chiara
Emiliani, Carla
Martino, Sabata
Latterini, Loredana
author_facet Tarpani, Luigi
Morena, Francesco
Gambucci, Marta
Zampini, Giulia
Massaro, Giuseppina
Argentati, Chiara
Emiliani, Carla
Martino, Sabata
Latterini, Loredana
author_sort Tarpani, Luigi
collection PubMed
description The preparation of tailored nanomaterials able to support cell growth and viability is mandatory for tissue engineering applications. In the present work, silica nanoparticles were prepared by a sol-gel procedure and were then functionalized by condensation of amino groups and by adsorption of silver nanoparticles. Transmission electron microscopy (TEM) imaging was used to establish the morphology and the average dimensions of about 130 nm, which were not affected by the functionalization. The three silica samples were deposited (1 mg/mL) on cover glasses, which were used as a substrate to culture adult human bone marrow-mesenchymal stem cells (hBM-MSCs) and human adipose-derived stem cells (hASCs). The good cell viability over the different silica surfaces was evaluated by monitoring the mitochondrial dehydrogenase activity. The analysis of the morphological parameters (aspect ratio, cell length, and nuclear shape Index) yielded information about the interactions of stem cells with the surface of three different nanoparticles. The data are discussed in terms of chemical properties of the surface of silica nanoparticles.
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spelling pubmed-53026272017-03-21 The Influence of Modified Silica Nanomaterials on Adult Stem Cell Culture Tarpani, Luigi Morena, Francesco Gambucci, Marta Zampini, Giulia Massaro, Giuseppina Argentati, Chiara Emiliani, Carla Martino, Sabata Latterini, Loredana Nanomaterials (Basel) Article The preparation of tailored nanomaterials able to support cell growth and viability is mandatory for tissue engineering applications. In the present work, silica nanoparticles were prepared by a sol-gel procedure and were then functionalized by condensation of amino groups and by adsorption of silver nanoparticles. Transmission electron microscopy (TEM) imaging was used to establish the morphology and the average dimensions of about 130 nm, which were not affected by the functionalization. The three silica samples were deposited (1 mg/mL) on cover glasses, which were used as a substrate to culture adult human bone marrow-mesenchymal stem cells (hBM-MSCs) and human adipose-derived stem cells (hASCs). The good cell viability over the different silica surfaces was evaluated by monitoring the mitochondrial dehydrogenase activity. The analysis of the morphological parameters (aspect ratio, cell length, and nuclear shape Index) yielded information about the interactions of stem cells with the surface of three different nanoparticles. The data are discussed in terms of chemical properties of the surface of silica nanoparticles. MDPI 2016-06-04 /pmc/articles/PMC5302627/ /pubmed/28335232 http://dx.doi.org/10.3390/nano6060104 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tarpani, Luigi
Morena, Francesco
Gambucci, Marta
Zampini, Giulia
Massaro, Giuseppina
Argentati, Chiara
Emiliani, Carla
Martino, Sabata
Latterini, Loredana
The Influence of Modified Silica Nanomaterials on Adult Stem Cell Culture
title The Influence of Modified Silica Nanomaterials on Adult Stem Cell Culture
title_full The Influence of Modified Silica Nanomaterials on Adult Stem Cell Culture
title_fullStr The Influence of Modified Silica Nanomaterials on Adult Stem Cell Culture
title_full_unstemmed The Influence of Modified Silica Nanomaterials on Adult Stem Cell Culture
title_short The Influence of Modified Silica Nanomaterials on Adult Stem Cell Culture
title_sort influence of modified silica nanomaterials on adult stem cell culture
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5302627/
https://www.ncbi.nlm.nih.gov/pubmed/28335232
http://dx.doi.org/10.3390/nano6060104
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