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Comparative and Quantitative Global Proteomics Approaches: An Overview

Proteomics became a key tool for the study of biological systems. The comparison between two different physiological states allows unravelling the cellular and molecular mechanisms involved in a biological process. Proteomics can confirm the presence of proteins suggested by their mRNA content and p...

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Autores principales: Deracinois, Barbara, Flahaut, Christophe, Duban-Deweer, Sophie, Karamanos, Yannis
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5302699/
https://www.ncbi.nlm.nih.gov/pubmed/28250403
http://dx.doi.org/10.3390/proteomes1030180
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author Deracinois, Barbara
Flahaut, Christophe
Duban-Deweer, Sophie
Karamanos, Yannis
author_facet Deracinois, Barbara
Flahaut, Christophe
Duban-Deweer, Sophie
Karamanos, Yannis
author_sort Deracinois, Barbara
collection PubMed
description Proteomics became a key tool for the study of biological systems. The comparison between two different physiological states allows unravelling the cellular and molecular mechanisms involved in a biological process. Proteomics can confirm the presence of proteins suggested by their mRNA content and provides a direct measure of the quantity present in a cell. Global and targeted proteomics strategies can be applied. Targeted proteomics strategies limit the number of features that will be monitored and then optimise the methods to obtain the highest sensitivity and throughput for a huge amount of samples. The advantage of global proteomics strategies is that no hypothesis is required, other than a measurable difference in one or more protein species between the samples. Global proteomics methods attempt to separate quantify and identify all the proteins from a given sample. This review highlights only the different techniques of separation and quantification of proteins and peptides, in view of a comparative and quantitative global proteomics analysis. The in-gel and off-gel quantification of proteins will be discussed as well as the corresponding mass spectrometry technology. The overview is focused on the widespread techniques while keeping in mind that each approach is modular and often recovers the other.
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spelling pubmed-53026992017-02-27 Comparative and Quantitative Global Proteomics Approaches: An Overview Deracinois, Barbara Flahaut, Christophe Duban-Deweer, Sophie Karamanos, Yannis Proteomes Review Proteomics became a key tool for the study of biological systems. The comparison between two different physiological states allows unravelling the cellular and molecular mechanisms involved in a biological process. Proteomics can confirm the presence of proteins suggested by their mRNA content and provides a direct measure of the quantity present in a cell. Global and targeted proteomics strategies can be applied. Targeted proteomics strategies limit the number of features that will be monitored and then optimise the methods to obtain the highest sensitivity and throughput for a huge amount of samples. The advantage of global proteomics strategies is that no hypothesis is required, other than a measurable difference in one or more protein species between the samples. Global proteomics methods attempt to separate quantify and identify all the proteins from a given sample. This review highlights only the different techniques of separation and quantification of proteins and peptides, in view of a comparative and quantitative global proteomics analysis. The in-gel and off-gel quantification of proteins will be discussed as well as the corresponding mass spectrometry technology. The overview is focused on the widespread techniques while keeping in mind that each approach is modular and often recovers the other. MDPI 2013-10-11 /pmc/articles/PMC5302699/ /pubmed/28250403 http://dx.doi.org/10.3390/proteomes1030180 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Review
Deracinois, Barbara
Flahaut, Christophe
Duban-Deweer, Sophie
Karamanos, Yannis
Comparative and Quantitative Global Proteomics Approaches: An Overview
title Comparative and Quantitative Global Proteomics Approaches: An Overview
title_full Comparative and Quantitative Global Proteomics Approaches: An Overview
title_fullStr Comparative and Quantitative Global Proteomics Approaches: An Overview
title_full_unstemmed Comparative and Quantitative Global Proteomics Approaches: An Overview
title_short Comparative and Quantitative Global Proteomics Approaches: An Overview
title_sort comparative and quantitative global proteomics approaches: an overview
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5302699/
https://www.ncbi.nlm.nih.gov/pubmed/28250403
http://dx.doi.org/10.3390/proteomes1030180
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