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An integrative framework to identify cell death-related microRNAs in esophageal squamous cell carcinoma
Cell death is a critical biological process involved in many important functions, and defects in this system are usually linked with numerous human diseases including cancers. Esophageal squamous cell carcinoma is one of the most chemo- and biological therapy resistant cancers. Based on knowledge re...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5302951/ https://www.ncbi.nlm.nih.gov/pubmed/27462775 http://dx.doi.org/10.18632/oncotarget.10779 |
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author | Wu, Bing-Li Wang, Dong Bai, Wen-Jing Zhang, Fan Zhao, Xing Yi, Ying Zhang, Ting Shen, Wen-Jun Li, En-Min Xu, Li-Yan Xu, Jian-Zhen |
author_facet | Wu, Bing-Li Wang, Dong Bai, Wen-Jing Zhang, Fan Zhao, Xing Yi, Ying Zhang, Ting Shen, Wen-Jun Li, En-Min Xu, Li-Yan Xu, Jian-Zhen |
author_sort | Wu, Bing-Li |
collection | PubMed |
description | Cell death is a critical biological process involved in many important functions, and defects in this system are usually linked with numerous human diseases including cancers. Esophageal squamous cell carcinoma is one of the most chemo- and biological therapy resistant cancers. Based on knowledge repository and four miRNAs profiling data, we proposed a general framework to hunt for cell death miRNAs in a context dependent manner. We predicted 12 candidate miRNAs from hundreds of others. Follow-up experimental verification of 7 miRNAs indicated at least 3 miRNAs (MIR20b, MIR498 and MIR196) were involved in both apoptosis and autophagy processes. These results indicated miRNAs intimately connected the two cell death modules in esophageal squamous cell carcinoma. This integrative framework can also be easily extended to identify miRNAs in other key cellular signaling pathways or may find conditional specific miRNAs in other cancer types. |
format | Online Article Text |
id | pubmed-5302951 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-53029512017-02-13 An integrative framework to identify cell death-related microRNAs in esophageal squamous cell carcinoma Wu, Bing-Li Wang, Dong Bai, Wen-Jing Zhang, Fan Zhao, Xing Yi, Ying Zhang, Ting Shen, Wen-Jun Li, En-Min Xu, Li-Yan Xu, Jian-Zhen Oncotarget Research Paper Cell death is a critical biological process involved in many important functions, and defects in this system are usually linked with numerous human diseases including cancers. Esophageal squamous cell carcinoma is one of the most chemo- and biological therapy resistant cancers. Based on knowledge repository and four miRNAs profiling data, we proposed a general framework to hunt for cell death miRNAs in a context dependent manner. We predicted 12 candidate miRNAs from hundreds of others. Follow-up experimental verification of 7 miRNAs indicated at least 3 miRNAs (MIR20b, MIR498 and MIR196) were involved in both apoptosis and autophagy processes. These results indicated miRNAs intimately connected the two cell death modules in esophageal squamous cell carcinoma. This integrative framework can also be easily extended to identify miRNAs in other key cellular signaling pathways or may find conditional specific miRNAs in other cancer types. Impact Journals LLC 2016-07-22 /pmc/articles/PMC5302951/ /pubmed/27462775 http://dx.doi.org/10.18632/oncotarget.10779 Text en Copyright: © 2016 Wu et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Wu, Bing-Li Wang, Dong Bai, Wen-Jing Zhang, Fan Zhao, Xing Yi, Ying Zhang, Ting Shen, Wen-Jun Li, En-Min Xu, Li-Yan Xu, Jian-Zhen An integrative framework to identify cell death-related microRNAs in esophageal squamous cell carcinoma |
title | An integrative framework to identify cell death-related microRNAs in esophageal squamous cell carcinoma |
title_full | An integrative framework to identify cell death-related microRNAs in esophageal squamous cell carcinoma |
title_fullStr | An integrative framework to identify cell death-related microRNAs in esophageal squamous cell carcinoma |
title_full_unstemmed | An integrative framework to identify cell death-related microRNAs in esophageal squamous cell carcinoma |
title_short | An integrative framework to identify cell death-related microRNAs in esophageal squamous cell carcinoma |
title_sort | integrative framework to identify cell death-related micrornas in esophageal squamous cell carcinoma |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5302951/ https://www.ncbi.nlm.nih.gov/pubmed/27462775 http://dx.doi.org/10.18632/oncotarget.10779 |
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