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A three-dimensional collagen scaffold cell culture system for screening anti-glioma therapeutics
Three-dimensional (3D) culture, which can simulate in vivo microenvironments, has been increasingly used to study tumor cell biology. Since most preclinical anti-glioma drug tests still rely on conventional 2D cell culture, we established a collagen scaffold for 3D glioma cell culture. Glioma cells...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5302961/ https://www.ncbi.nlm.nih.gov/pubmed/27486877 http://dx.doi.org/10.18632/oncotarget.10885 |
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author | Lv, Donglai Yu, Shi-cang Ping, Yi-fang Wu, Haibo Zhao, Xilong Zhang, Huarong Cui, Youhong Chen, Bing Zhang, Xia Dai, Jianwu Bian, Xiu-wu Yao, Xiao-hong |
author_facet | Lv, Donglai Yu, Shi-cang Ping, Yi-fang Wu, Haibo Zhao, Xilong Zhang, Huarong Cui, Youhong Chen, Bing Zhang, Xia Dai, Jianwu Bian, Xiu-wu Yao, Xiao-hong |
author_sort | Lv, Donglai |
collection | PubMed |
description | Three-dimensional (3D) culture, which can simulate in vivo microenvironments, has been increasingly used to study tumor cell biology. Since most preclinical anti-glioma drug tests still rely on conventional 2D cell culture, we established a collagen scaffold for 3D glioma cell culture. Glioma cells cultured on these 3D scaffolds showed greater degree of dedifferentiation and quiescence than cells in 2D culture. 3D-cultured cells also exhibited enhanced resistance to chemotherapeutic alkylating agents, with a much higher proportion of glioma stem cells and upregulation of O6-methylguanine DNA methyltransferase (MGMT). Importantly, tumor cells in 3D culture showed chemotherapy resistance patterns similar to those observed in glioma patients. Our results suggest that 3D collagen scaffolds are promising in vitro research platforms for screening new anti-glioma therapeutics. |
format | Online Article Text |
id | pubmed-5302961 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-53029612017-02-13 A three-dimensional collagen scaffold cell culture system for screening anti-glioma therapeutics Lv, Donglai Yu, Shi-cang Ping, Yi-fang Wu, Haibo Zhao, Xilong Zhang, Huarong Cui, Youhong Chen, Bing Zhang, Xia Dai, Jianwu Bian, Xiu-wu Yao, Xiao-hong Oncotarget Research Paper Three-dimensional (3D) culture, which can simulate in vivo microenvironments, has been increasingly used to study tumor cell biology. Since most preclinical anti-glioma drug tests still rely on conventional 2D cell culture, we established a collagen scaffold for 3D glioma cell culture. Glioma cells cultured on these 3D scaffolds showed greater degree of dedifferentiation and quiescence than cells in 2D culture. 3D-cultured cells also exhibited enhanced resistance to chemotherapeutic alkylating agents, with a much higher proportion of glioma stem cells and upregulation of O6-methylguanine DNA methyltransferase (MGMT). Importantly, tumor cells in 3D culture showed chemotherapy resistance patterns similar to those observed in glioma patients. Our results suggest that 3D collagen scaffolds are promising in vitro research platforms for screening new anti-glioma therapeutics. Impact Journals LLC 2016-07-28 /pmc/articles/PMC5302961/ /pubmed/27486877 http://dx.doi.org/10.18632/oncotarget.10885 Text en Copyright: © 2016 Lv et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Lv, Donglai Yu, Shi-cang Ping, Yi-fang Wu, Haibo Zhao, Xilong Zhang, Huarong Cui, Youhong Chen, Bing Zhang, Xia Dai, Jianwu Bian, Xiu-wu Yao, Xiao-hong A three-dimensional collagen scaffold cell culture system for screening anti-glioma therapeutics |
title | A three-dimensional collagen scaffold cell culture system for screening anti-glioma therapeutics |
title_full | A three-dimensional collagen scaffold cell culture system for screening anti-glioma therapeutics |
title_fullStr | A three-dimensional collagen scaffold cell culture system for screening anti-glioma therapeutics |
title_full_unstemmed | A three-dimensional collagen scaffold cell culture system for screening anti-glioma therapeutics |
title_short | A three-dimensional collagen scaffold cell culture system for screening anti-glioma therapeutics |
title_sort | three-dimensional collagen scaffold cell culture system for screening anti-glioma therapeutics |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5302961/ https://www.ncbi.nlm.nih.gov/pubmed/27486877 http://dx.doi.org/10.18632/oncotarget.10885 |
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