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A three-dimensional collagen scaffold cell culture system for screening anti-glioma therapeutics

Three-dimensional (3D) culture, which can simulate in vivo microenvironments, has been increasingly used to study tumor cell biology. Since most preclinical anti-glioma drug tests still rely on conventional 2D cell culture, we established a collagen scaffold for 3D glioma cell culture. Glioma cells...

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Autores principales: Lv, Donglai, Yu, Shi-cang, Ping, Yi-fang, Wu, Haibo, Zhao, Xilong, Zhang, Huarong, Cui, Youhong, Chen, Bing, Zhang, Xia, Dai, Jianwu, Bian, Xiu-wu, Yao, Xiao-hong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5302961/
https://www.ncbi.nlm.nih.gov/pubmed/27486877
http://dx.doi.org/10.18632/oncotarget.10885
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author Lv, Donglai
Yu, Shi-cang
Ping, Yi-fang
Wu, Haibo
Zhao, Xilong
Zhang, Huarong
Cui, Youhong
Chen, Bing
Zhang, Xia
Dai, Jianwu
Bian, Xiu-wu
Yao, Xiao-hong
author_facet Lv, Donglai
Yu, Shi-cang
Ping, Yi-fang
Wu, Haibo
Zhao, Xilong
Zhang, Huarong
Cui, Youhong
Chen, Bing
Zhang, Xia
Dai, Jianwu
Bian, Xiu-wu
Yao, Xiao-hong
author_sort Lv, Donglai
collection PubMed
description Three-dimensional (3D) culture, which can simulate in vivo microenvironments, has been increasingly used to study tumor cell biology. Since most preclinical anti-glioma drug tests still rely on conventional 2D cell culture, we established a collagen scaffold for 3D glioma cell culture. Glioma cells cultured on these 3D scaffolds showed greater degree of dedifferentiation and quiescence than cells in 2D culture. 3D-cultured cells also exhibited enhanced resistance to chemotherapeutic alkylating agents, with a much higher proportion of glioma stem cells and upregulation of O6-methylguanine DNA methyltransferase (MGMT). Importantly, tumor cells in 3D culture showed chemotherapy resistance patterns similar to those observed in glioma patients. Our results suggest that 3D collagen scaffolds are promising in vitro research platforms for screening new anti-glioma therapeutics.
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spelling pubmed-53029612017-02-13 A three-dimensional collagen scaffold cell culture system for screening anti-glioma therapeutics Lv, Donglai Yu, Shi-cang Ping, Yi-fang Wu, Haibo Zhao, Xilong Zhang, Huarong Cui, Youhong Chen, Bing Zhang, Xia Dai, Jianwu Bian, Xiu-wu Yao, Xiao-hong Oncotarget Research Paper Three-dimensional (3D) culture, which can simulate in vivo microenvironments, has been increasingly used to study tumor cell biology. Since most preclinical anti-glioma drug tests still rely on conventional 2D cell culture, we established a collagen scaffold for 3D glioma cell culture. Glioma cells cultured on these 3D scaffolds showed greater degree of dedifferentiation and quiescence than cells in 2D culture. 3D-cultured cells also exhibited enhanced resistance to chemotherapeutic alkylating agents, with a much higher proportion of glioma stem cells and upregulation of O6-methylguanine DNA methyltransferase (MGMT). Importantly, tumor cells in 3D culture showed chemotherapy resistance patterns similar to those observed in glioma patients. Our results suggest that 3D collagen scaffolds are promising in vitro research platforms for screening new anti-glioma therapeutics. Impact Journals LLC 2016-07-28 /pmc/articles/PMC5302961/ /pubmed/27486877 http://dx.doi.org/10.18632/oncotarget.10885 Text en Copyright: © 2016 Lv et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Lv, Donglai
Yu, Shi-cang
Ping, Yi-fang
Wu, Haibo
Zhao, Xilong
Zhang, Huarong
Cui, Youhong
Chen, Bing
Zhang, Xia
Dai, Jianwu
Bian, Xiu-wu
Yao, Xiao-hong
A three-dimensional collagen scaffold cell culture system for screening anti-glioma therapeutics
title A three-dimensional collagen scaffold cell culture system for screening anti-glioma therapeutics
title_full A three-dimensional collagen scaffold cell culture system for screening anti-glioma therapeutics
title_fullStr A three-dimensional collagen scaffold cell culture system for screening anti-glioma therapeutics
title_full_unstemmed A three-dimensional collagen scaffold cell culture system for screening anti-glioma therapeutics
title_short A three-dimensional collagen scaffold cell culture system for screening anti-glioma therapeutics
title_sort three-dimensional collagen scaffold cell culture system for screening anti-glioma therapeutics
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5302961/
https://www.ncbi.nlm.nih.gov/pubmed/27486877
http://dx.doi.org/10.18632/oncotarget.10885
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