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Biocompatibility of Mineral Trioxide Aggregate with TiO2 Nanoparticles on Human Gingival Fibroblasts

BACKGROUND: The New compositions of white mineral trioxide aggregate (WMTA) or use of various additives like nanoparticles might affect MTA’s ideal characteristics This study was performed to evaluate the cytotoxicity of WMTA and WMTA with Titanium dioxide (TiO2) nanoparticles (1% weight ratio) at d...

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Autores principales: Samiei, Mohammad, Ghasemi, Negin, Aghazadeh, Marzieh, Divband, Baharak, Akbarzadeh, Farzaneh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medicina Oral S.L. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5303314/
https://www.ncbi.nlm.nih.gov/pubmed/28210432
http://dx.doi.org/10.4317/jced.53126
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author Samiei, Mohammad
Ghasemi, Negin
Aghazadeh, Marzieh
Divband, Baharak
Akbarzadeh, Farzaneh
author_facet Samiei, Mohammad
Ghasemi, Negin
Aghazadeh, Marzieh
Divband, Baharak
Akbarzadeh, Farzaneh
author_sort Samiei, Mohammad
collection PubMed
description BACKGROUND: The New compositions of white mineral trioxide aggregate (WMTA) or use of various additives like nanoparticles might affect MTA’s ideal characteristics This study was performed to evaluate the cytotoxicity of WMTA and WMTA with Titanium dioxide (TiO2) nanoparticles (1% weight ratio) at different storage times after mixing on human gingival fibroblasts (HGFs). MATERIAL AND METHODS: HGFs were obtained from the attached gingiva of human premolars. HGFs were cultured in Dulbecco’s Modified Eagle medium, supplemented with 10% fetal calf serum, penicillin and streptomycin. The cells were exposed to WMTA (groups 1 and 2) and WMTA+TiO2 (groups 3 and 4). The fifth and sixth groups served as controls. Each group contained 15 wells. After 24h (groups 1, 3 and 5) and 48 h (groups 2, 4 and 6) of exposure, HGF viability was determined by Mosmann’s tetrazolium toxicity (MTT) assay. Statistical analysis of the data was performed by using one-way analysis of variance and Tukey post hoc test, with significance of p < 0.05. RESULTS: With both materials, the viability of HGFs significantly decrased with increasing the incubation time from 24h to 48 h (P<0.05). There was no significant difference between the materials regarding HGF viability (P>0.05). CONCLUSIONS: Under the limitations of the present study, incorporation of TiO2 nanoparticles into MTA at 1 wt% had no negative effect on its biocompatibility. Key words:Cytotoxicity, fibroblast, MTA, MTT assay, nanoparticle, TiO2.
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spelling pubmed-53033142017-02-16 Biocompatibility of Mineral Trioxide Aggregate with TiO2 Nanoparticles on Human Gingival Fibroblasts Samiei, Mohammad Ghasemi, Negin Aghazadeh, Marzieh Divband, Baharak Akbarzadeh, Farzaneh J Clin Exp Dent Research BACKGROUND: The New compositions of white mineral trioxide aggregate (WMTA) or use of various additives like nanoparticles might affect MTA’s ideal characteristics This study was performed to evaluate the cytotoxicity of WMTA and WMTA with Titanium dioxide (TiO2) nanoparticles (1% weight ratio) at different storage times after mixing on human gingival fibroblasts (HGFs). MATERIAL AND METHODS: HGFs were obtained from the attached gingiva of human premolars. HGFs were cultured in Dulbecco’s Modified Eagle medium, supplemented with 10% fetal calf serum, penicillin and streptomycin. The cells were exposed to WMTA (groups 1 and 2) and WMTA+TiO2 (groups 3 and 4). The fifth and sixth groups served as controls. Each group contained 15 wells. After 24h (groups 1, 3 and 5) and 48 h (groups 2, 4 and 6) of exposure, HGF viability was determined by Mosmann’s tetrazolium toxicity (MTT) assay. Statistical analysis of the data was performed by using one-way analysis of variance and Tukey post hoc test, with significance of p < 0.05. RESULTS: With both materials, the viability of HGFs significantly decrased with increasing the incubation time from 24h to 48 h (P<0.05). There was no significant difference between the materials regarding HGF viability (P>0.05). CONCLUSIONS: Under the limitations of the present study, incorporation of TiO2 nanoparticles into MTA at 1 wt% had no negative effect on its biocompatibility. Key words:Cytotoxicity, fibroblast, MTA, MTT assay, nanoparticle, TiO2. Medicina Oral S.L. 2017-02-01 /pmc/articles/PMC5303314/ /pubmed/28210432 http://dx.doi.org/10.4317/jced.53126 Text en Copyright: © 2017 Medicina Oral S.L. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Samiei, Mohammad
Ghasemi, Negin
Aghazadeh, Marzieh
Divband, Baharak
Akbarzadeh, Farzaneh
Biocompatibility of Mineral Trioxide Aggregate with TiO2 Nanoparticles on Human Gingival Fibroblasts
title Biocompatibility of Mineral Trioxide Aggregate with TiO2 Nanoparticles on Human Gingival Fibroblasts
title_full Biocompatibility of Mineral Trioxide Aggregate with TiO2 Nanoparticles on Human Gingival Fibroblasts
title_fullStr Biocompatibility of Mineral Trioxide Aggregate with TiO2 Nanoparticles on Human Gingival Fibroblasts
title_full_unstemmed Biocompatibility of Mineral Trioxide Aggregate with TiO2 Nanoparticles on Human Gingival Fibroblasts
title_short Biocompatibility of Mineral Trioxide Aggregate with TiO2 Nanoparticles on Human Gingival Fibroblasts
title_sort biocompatibility of mineral trioxide aggregate with tio2 nanoparticles on human gingival fibroblasts
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5303314/
https://www.ncbi.nlm.nih.gov/pubmed/28210432
http://dx.doi.org/10.4317/jced.53126
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