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Evaluation of GeneXpert MTB/RIF for detection of Mycobacterium tuberculosis complex and rpo B gene in respiratory and non-respiratory clinical specimens at a tertiary care teaching hospital in Saudi Arabia

OBJECTIVES: To assess the performance of Xpert MTB/RIF, an automated molecular test for Mycobacterium tuberculosis (MTB) and resistance to rifampin (RIF), against smear microscopy and culture method for diagnosis of MTB infection. METHODS: This is a retrospective analysis of 103 respiratory and 137...

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Autores principales: Somily, Ali M., Barry, Mazin A., Habib, Hanan A., Alotaibi, Fawzia E., Al-Zamil, Fahad A., Khan, Mohammed A., Sarwar, Mohammed S., Bakhash, Nawab D., Alrabiaah, Abdulkarim A., Shakoor, Zahid A., Senok, Abiola C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Saudi Medical Journal 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5303782/
https://www.ncbi.nlm.nih.gov/pubmed/27874159
http://dx.doi.org/10.15537/smj.2016.12.15506
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author Somily, Ali M.
Barry, Mazin A.
Habib, Hanan A.
Alotaibi, Fawzia E.
Al-Zamil, Fahad A.
Khan, Mohammed A.
Sarwar, Mohammed S.
Bakhash, Nawab D.
Alrabiaah, Abdulkarim A.
Shakoor, Zahid A.
Senok, Abiola C.
author_facet Somily, Ali M.
Barry, Mazin A.
Habib, Hanan A.
Alotaibi, Fawzia E.
Al-Zamil, Fahad A.
Khan, Mohammed A.
Sarwar, Mohammed S.
Bakhash, Nawab D.
Alrabiaah, Abdulkarim A.
Shakoor, Zahid A.
Senok, Abiola C.
author_sort Somily, Ali M.
collection PubMed
description OBJECTIVES: To assess the performance of Xpert MTB/RIF, an automated molecular test for Mycobacterium tuberculosis (MTB) and resistance to rifampin (RIF), against smear microscopy and culture method for diagnosis of MTB infection. METHODS: This is a retrospective analysis of 103 respiratory and 137 non-respiratory patient specimens suspected of tuberculosis at King Khalid University Hospital, Riyadh, Kingdom of Saudi Arabia performed between April 2014 and March 2015. Each sample underwent smear microscopy, mycobacterial culture, and GeneXpert MTB/RIF test. RESULTS: Fifteen out of 103 respiratory samples were smear and culture positive, whereas 9 out of 137 non-respiratory samples were smear positive. Out of 9 smear positive specimens, 8 were also culture positive. All 15 culture positive respiratory samples were detected by Xpert MTB/RIF (sensitivity and positive predictive value [PPV]=100%). Similarly, all 8 culture positive non-respiratory specimens were identified by Xpert MTB/RIF (sensitivity 100%; PPV 88.8%). The Xpert MTB/RIF detected only one false positive result in 88 smear negative respiratory specimens (specificity 98.9%; negative predictive value [NPV]= 100%). All 125 smear negative non-respiratory specimens tested negative by culture and Xpert MTB/RIF (sensitivity, specificity, PPV, NPV= 100%). CONCLUSION: The performance of Xpert MTB/RIF was comparable to the gold standard culture method for identification of MTB in both respiratory and non-respiratory clinical specimens.
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spelling pubmed-53037822017-02-16 Evaluation of GeneXpert MTB/RIF for detection of Mycobacterium tuberculosis complex and rpo B gene in respiratory and non-respiratory clinical specimens at a tertiary care teaching hospital in Saudi Arabia Somily, Ali M. Barry, Mazin A. Habib, Hanan A. Alotaibi, Fawzia E. Al-Zamil, Fahad A. Khan, Mohammed A. Sarwar, Mohammed S. Bakhash, Nawab D. Alrabiaah, Abdulkarim A. Shakoor, Zahid A. Senok, Abiola C. Saudi Med J Brief Communication OBJECTIVES: To assess the performance of Xpert MTB/RIF, an automated molecular test for Mycobacterium tuberculosis (MTB) and resistance to rifampin (RIF), against smear microscopy and culture method for diagnosis of MTB infection. METHODS: This is a retrospective analysis of 103 respiratory and 137 non-respiratory patient specimens suspected of tuberculosis at King Khalid University Hospital, Riyadh, Kingdom of Saudi Arabia performed between April 2014 and March 2015. Each sample underwent smear microscopy, mycobacterial culture, and GeneXpert MTB/RIF test. RESULTS: Fifteen out of 103 respiratory samples were smear and culture positive, whereas 9 out of 137 non-respiratory samples were smear positive. Out of 9 smear positive specimens, 8 were also culture positive. All 15 culture positive respiratory samples were detected by Xpert MTB/RIF (sensitivity and positive predictive value [PPV]=100%). Similarly, all 8 culture positive non-respiratory specimens were identified by Xpert MTB/RIF (sensitivity 100%; PPV 88.8%). The Xpert MTB/RIF detected only one false positive result in 88 smear negative respiratory specimens (specificity 98.9%; negative predictive value [NPV]= 100%). All 125 smear negative non-respiratory specimens tested negative by culture and Xpert MTB/RIF (sensitivity, specificity, PPV, NPV= 100%). CONCLUSION: The performance of Xpert MTB/RIF was comparable to the gold standard culture method for identification of MTB in both respiratory and non-respiratory clinical specimens. Saudi Medical Journal 2016-12 /pmc/articles/PMC5303782/ /pubmed/27874159 http://dx.doi.org/10.15537/smj.2016.12.15506 Text en Copyright: © Saudi Medical Journal http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Brief Communication
Somily, Ali M.
Barry, Mazin A.
Habib, Hanan A.
Alotaibi, Fawzia E.
Al-Zamil, Fahad A.
Khan, Mohammed A.
Sarwar, Mohammed S.
Bakhash, Nawab D.
Alrabiaah, Abdulkarim A.
Shakoor, Zahid A.
Senok, Abiola C.
Evaluation of GeneXpert MTB/RIF for detection of Mycobacterium tuberculosis complex and rpo B gene in respiratory and non-respiratory clinical specimens at a tertiary care teaching hospital in Saudi Arabia
title Evaluation of GeneXpert MTB/RIF for detection of Mycobacterium tuberculosis complex and rpo B gene in respiratory and non-respiratory clinical specimens at a tertiary care teaching hospital in Saudi Arabia
title_full Evaluation of GeneXpert MTB/RIF for detection of Mycobacterium tuberculosis complex and rpo B gene in respiratory and non-respiratory clinical specimens at a tertiary care teaching hospital in Saudi Arabia
title_fullStr Evaluation of GeneXpert MTB/RIF for detection of Mycobacterium tuberculosis complex and rpo B gene in respiratory and non-respiratory clinical specimens at a tertiary care teaching hospital in Saudi Arabia
title_full_unstemmed Evaluation of GeneXpert MTB/RIF for detection of Mycobacterium tuberculosis complex and rpo B gene in respiratory and non-respiratory clinical specimens at a tertiary care teaching hospital in Saudi Arabia
title_short Evaluation of GeneXpert MTB/RIF for detection of Mycobacterium tuberculosis complex and rpo B gene in respiratory and non-respiratory clinical specimens at a tertiary care teaching hospital in Saudi Arabia
title_sort evaluation of genexpert mtb/rif for detection of mycobacterium tuberculosis complex and rpo b gene in respiratory and non-respiratory clinical specimens at a tertiary care teaching hospital in saudi arabia
topic Brief Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5303782/
https://www.ncbi.nlm.nih.gov/pubmed/27874159
http://dx.doi.org/10.15537/smj.2016.12.15506
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