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Molecular Characteristics and Antibiotic Resistance Profiles of Klebsiella Isolates in Mthatha, Eastern Cape Province, South Africa
The increase in the incidence of extended-spectrum β-lactamase- (ESBL-) producing Klebsiella species has become a serious problem worldwide, because of their incrimination in antibiotic resistance. The objective of this study is to investigate the resistance genes responsible for ESBL-producing Kleb...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Hindawi Publishing Corporation
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5303861/ https://www.ncbi.nlm.nih.gov/pubmed/28250772 http://dx.doi.org/10.1155/2017/8486742 |
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author | Vasaikar, Sandeep Obi, Larry Morobe, Isaac Bisi-Johnson, Mary |
author_facet | Vasaikar, Sandeep Obi, Larry Morobe, Isaac Bisi-Johnson, Mary |
author_sort | Vasaikar, Sandeep |
collection | PubMed |
description | The increase in the incidence of extended-spectrum β-lactamase- (ESBL-) producing Klebsiella species has become a serious problem worldwide, because of their incrimination in antibiotic resistance. The objective of this study is to investigate the resistance genes responsible for ESBL-producing Klebsiella species and carbapenemase-producing Klebsiella (CRE) isolated in Mthatha and to study their epidemiology. A prospective, descriptive study of 202 nonrepetitive samples from patients was obtained from Nelson Mandela Academic Hospital. The cultured Klebsiella isolates were subjected to antimicrobial susceptibility tests and the polymerase chain reaction of bla(CTX-M), bla(TEM), bla(SHV), bla(KPC), and bla(NDM) genes. Overall K. pneumoniae were the majority with 169 (83.7%) species isolates, followed by K. oxytoca with 29 (14.4%), while K. ozaenae and Raoultella ornithinolytica were 2 (0.9%) each. The prevalence of ESBL production in all Klebsiella species was 117 (57.9%). ESBL-genotypic resistance is driven in Mthatha by bla(SHV) 121 (77.1%) followed by bla(TEM) 105 (66.9%) and bla(CTX-M) at 89 (56.7%). The most common ESBL genotype combination among the Klebsiella was bla(TEM) + bla(SHV) + bla(CTX-M) at 79 (50.3%). There is a steady increase in the rate of ESBL genes in the last five years. |
format | Online Article Text |
id | pubmed-5303861 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-53038612017-03-01 Molecular Characteristics and Antibiotic Resistance Profiles of Klebsiella Isolates in Mthatha, Eastern Cape Province, South Africa Vasaikar, Sandeep Obi, Larry Morobe, Isaac Bisi-Johnson, Mary Int J Microbiol Research Article The increase in the incidence of extended-spectrum β-lactamase- (ESBL-) producing Klebsiella species has become a serious problem worldwide, because of their incrimination in antibiotic resistance. The objective of this study is to investigate the resistance genes responsible for ESBL-producing Klebsiella species and carbapenemase-producing Klebsiella (CRE) isolated in Mthatha and to study their epidemiology. A prospective, descriptive study of 202 nonrepetitive samples from patients was obtained from Nelson Mandela Academic Hospital. The cultured Klebsiella isolates were subjected to antimicrobial susceptibility tests and the polymerase chain reaction of bla(CTX-M), bla(TEM), bla(SHV), bla(KPC), and bla(NDM) genes. Overall K. pneumoniae were the majority with 169 (83.7%) species isolates, followed by K. oxytoca with 29 (14.4%), while K. ozaenae and Raoultella ornithinolytica were 2 (0.9%) each. The prevalence of ESBL production in all Klebsiella species was 117 (57.9%). ESBL-genotypic resistance is driven in Mthatha by bla(SHV) 121 (77.1%) followed by bla(TEM) 105 (66.9%) and bla(CTX-M) at 89 (56.7%). The most common ESBL genotype combination among the Klebsiella was bla(TEM) + bla(SHV) + bla(CTX-M) at 79 (50.3%). There is a steady increase in the rate of ESBL genes in the last five years. Hindawi Publishing Corporation 2017 2017-01-30 /pmc/articles/PMC5303861/ /pubmed/28250772 http://dx.doi.org/10.1155/2017/8486742 Text en Copyright © 2017 Sandeep Vasaikar et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Vasaikar, Sandeep Obi, Larry Morobe, Isaac Bisi-Johnson, Mary Molecular Characteristics and Antibiotic Resistance Profiles of Klebsiella Isolates in Mthatha, Eastern Cape Province, South Africa |
title | Molecular Characteristics and Antibiotic Resistance Profiles of Klebsiella Isolates in Mthatha, Eastern Cape Province, South Africa |
title_full | Molecular Characteristics and Antibiotic Resistance Profiles of Klebsiella Isolates in Mthatha, Eastern Cape Province, South Africa |
title_fullStr | Molecular Characteristics and Antibiotic Resistance Profiles of Klebsiella Isolates in Mthatha, Eastern Cape Province, South Africa |
title_full_unstemmed | Molecular Characteristics and Antibiotic Resistance Profiles of Klebsiella Isolates in Mthatha, Eastern Cape Province, South Africa |
title_short | Molecular Characteristics and Antibiotic Resistance Profiles of Klebsiella Isolates in Mthatha, Eastern Cape Province, South Africa |
title_sort | molecular characteristics and antibiotic resistance profiles of klebsiella isolates in mthatha, eastern cape province, south africa |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5303861/ https://www.ncbi.nlm.nih.gov/pubmed/28250772 http://dx.doi.org/10.1155/2017/8486742 |
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