Conjugation of Polymer-Coated Gold Nanoparticles with Antibodies—Synthesis and Characterization

The synthesis of polymer-coated gold nanoparticles with high colloidal stability is described, together with appropriate characterization techniques concerning the colloidal properties of the nanoparticles. Antibodies against vascular endothelial growth factor (VEGF) are conjugated to the surface of...

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Autores principales: Tan, Gamze, Kantner, Karsten, Zhang, Qian, Soliman, Mahmoud G., del Pino, Pablo, Parak, Wolfgang J., Onur, Mehmet A., Valdeperez, Daniel, Rejman, Joanna, Pelaz, Beatriz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5304631/
https://www.ncbi.nlm.nih.gov/pubmed/28347065
http://dx.doi.org/10.3390/nano5031297
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author Tan, Gamze
Kantner, Karsten
Zhang, Qian
Soliman, Mahmoud G.
del Pino, Pablo
Parak, Wolfgang J.
Onur, Mehmet A.
Valdeperez, Daniel
Rejman, Joanna
Pelaz, Beatriz
author_facet Tan, Gamze
Kantner, Karsten
Zhang, Qian
Soliman, Mahmoud G.
del Pino, Pablo
Parak, Wolfgang J.
Onur, Mehmet A.
Valdeperez, Daniel
Rejman, Joanna
Pelaz, Beatriz
author_sort Tan, Gamze
collection PubMed
description The synthesis of polymer-coated gold nanoparticles with high colloidal stability is described, together with appropriate characterization techniques concerning the colloidal properties of the nanoparticles. Antibodies against vascular endothelial growth factor (VEGF) are conjugated to the surface of the nanoparticles. Antibody attachment is probed by different techniques, giving a guideline about the characterization of such conjugates. The effect of the nanoparticles on human adenocarcinoma alveolar basal epithelial cells (A549) and human umbilical vein endothelial cells (HUVECs) is probed in terms of internalization and viability assays.
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spelling pubmed-53046312017-03-21 Conjugation of Polymer-Coated Gold Nanoparticles with Antibodies—Synthesis and Characterization Tan, Gamze Kantner, Karsten Zhang, Qian Soliman, Mahmoud G. del Pino, Pablo Parak, Wolfgang J. Onur, Mehmet A. Valdeperez, Daniel Rejman, Joanna Pelaz, Beatriz Nanomaterials (Basel) Article The synthesis of polymer-coated gold nanoparticles with high colloidal stability is described, together with appropriate characterization techniques concerning the colloidal properties of the nanoparticles. Antibodies against vascular endothelial growth factor (VEGF) are conjugated to the surface of the nanoparticles. Antibody attachment is probed by different techniques, giving a guideline about the characterization of such conjugates. The effect of the nanoparticles on human adenocarcinoma alveolar basal epithelial cells (A549) and human umbilical vein endothelial cells (HUVECs) is probed in terms of internalization and viability assays. MDPI 2015-08-07 /pmc/articles/PMC5304631/ /pubmed/28347065 http://dx.doi.org/10.3390/nano5031297 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tan, Gamze
Kantner, Karsten
Zhang, Qian
Soliman, Mahmoud G.
del Pino, Pablo
Parak, Wolfgang J.
Onur, Mehmet A.
Valdeperez, Daniel
Rejman, Joanna
Pelaz, Beatriz
Conjugation of Polymer-Coated Gold Nanoparticles with Antibodies—Synthesis and Characterization
title Conjugation of Polymer-Coated Gold Nanoparticles with Antibodies—Synthesis and Characterization
title_full Conjugation of Polymer-Coated Gold Nanoparticles with Antibodies—Synthesis and Characterization
title_fullStr Conjugation of Polymer-Coated Gold Nanoparticles with Antibodies—Synthesis and Characterization
title_full_unstemmed Conjugation of Polymer-Coated Gold Nanoparticles with Antibodies—Synthesis and Characterization
title_short Conjugation of Polymer-Coated Gold Nanoparticles with Antibodies—Synthesis and Characterization
title_sort conjugation of polymer-coated gold nanoparticles with antibodies—synthesis and characterization
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5304631/
https://www.ncbi.nlm.nih.gov/pubmed/28347065
http://dx.doi.org/10.3390/nano5031297
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