Cargando…

A novel RNA sequencing data analysis method for cell line authentication

We have developed a novel analysis method that can interrogate the authenticity of biological samples used for generation of transcriptome profiles in public data repositories. The method uses RNA sequencing information to reveal mutations in expressed transcripts and subsequently confirms the ident...

Descripción completa

Detalles Bibliográficos
Autores principales:	Fasterius, Erik, Raso, Cinzia, Kennedy, Susan, Rauch, Nora, Lundin, Pär, Kolch, Walter, Uhlén, Mathias, Al-Khalili Szigyarto, Cristina
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2017
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5305277/ https://www.ncbi.nlm.nih.gov/pubmed/28192450 http://dx.doi.org/10.1371/journal.pone.0171435

Ejemplares similares

Single-cell RNA-seq variant analysis for exploration of genetic heterogeneity in cancer
por: Fasterius, Erik, et al.
Publicado: (2019)

Analysis of public RNA-sequencing data reveals biological consequences of genetic heterogeneity in cell line populations
por: Fasterius, Erik, et al.
Publicado: (2018)

Poly (ADP-ribose) polymerase (PARP) is not involved in base excision repair but PARP inhibition traps a single-strand intermediate
por: Ström, Cecilia E., et al.
Publicado: (2011)

Transcriptional and metabolic rewiring of colorectal cancer cells expressing the oncogenic KRAS(G13D) mutation
por: Charitou, Theodosia, et al.
Publicado: (2019)

Transcriptome profiling of the interconnection of pathways involved in malignant transformation and response to hypoxia
por: Danielsson, Frida, et al.
Publicado: (2018)

Orthogonal proteomics methods warrant the development of Duchenne muscular dystrophy biomarkers
por: Johansson, Camilla, et al.
Publicado: (2023)

Biomarkers of Duchenne muscular dystrophy: current findings
por: Szigyarto, Cristina Al-Khalili, et al.
Publicado: (2018)

Extensive rewiring of the EGFR network in colorectal cancer cells expressing transforming levels of KRAS(G13D)
por: Kennedy, Susan A., et al.
Publicado: (2020)

Penalized regression calibration: A method for the prediction of survival outcomes using complex longitudinal and high‐dimensional data
por: Signorelli, Mirko, et al.
Publicado: (2021)

Blood-derived biomarkers correlate with clinical progression in Duchenne muscular dystrophy
por: Strandberg, Kristin, et al.
Publicado: (2020)

On authentication of cell lines
por: The Editors of Molecular Vision,
Publicado: (2013)

A systematic analysis of signaling reactivation and drug resistance
por: Kholodenko, Boris N., et al.
Publicado: (2021)

Proteomic Mapping of the Interactome of KRAS Mutants Identifies New Features of RAS Signalling Networks and the Mechanism of Action of Sotorasib
por: Nolan, Aoife, et al.
Publicado: (2023)

Mouse cell line authentication
por: Almeida, Jamie L., et al.
Publicado: (2013)

On-Beads Digestion in Conjunction with Data-Dependent Mass Spectrometry: A Shortcut to Quantitative and Dynamic Interaction Proteomics
por: Turriziani, Benedetta, et al.
Publicado: (2014)

Interactome dynamics of RAF1-BRAF kinase monomers and dimers
por: Iglesias-Martinez, Luis F., et al.
Publicado: (2023)

High-throughput sequencing for species authentication and contamination detection of 63 cell lines
por: Lung, Oliver, et al.
Publicado: (2021)

Standards for Cell Line Authentication and Beyond
por: Almeida, Jamie L., et al.
Publicado: (2016)

Enhanced validation of antibodies for research applications
por: Edfors, Fredrik, et al.
Publicado: (2018)

Cell Type-Specific Activation of AKT and ERK Signaling Pathways by Small Negatively-Charged Magnetic Nanoparticles
por: Rauch, Jens, et al.
Publicado: (2012)

Targeting MAPK Signaling in Cancer: Mechanisms of Drug Resistance and Sensitivity
por: Lee, Shannon, et al.
Publicado: (2020)

Short tandem repeat profiling via next-generation sequencing for cell line authentication
por: Chen, Yi-Hsien, et al.
Publicado: (2023)

Authentication, characterization and contamination detection of cell lines, xenografts and organoids by barcode deep NGS sequencing
por: Chen, Xiaobo, et al.
Publicado: (2020)

Comprehensive analysis of the genome transcriptome and proteome landscapes of three tumor cell lines
por: Akan, Pelin, et al.
Publicado: (2012)

The secret life of kinases: functions beyond catalysis
por: Rauch, Jens, et al.
Publicado: (2011)

Secure Command Line Solution for Token-based Authentication
por: Dykstra, Dave
Publicado: (2021)

Establishment, authenticity, and characterization of cervical cancer cell lines
por: Zuñiga Martinez, Ma de Lourdes, et al.
Publicado: (2022)

CCLHunter: An efficient toolkit for cancer cell line authentication
por: Bu, Congfan, et al.
Publicado: (2023)

On the authenticity of COVID-19 case figures
por: Kennedy, Adrian Patrick, et al.
Publicado: (2020)

Characterization of hemocytes and hematopoietic cells of a freshwater crayfish based on single-cell transcriptome analysis
por: Söderhäll, Irene, et al.
Publicado: (2022)

Authentication of the R06E Fruit Bat Cell Line
por: Jordan, Ingo, et al.
Publicado: (2012)

Cell line authentication: a necessity for reproducible biomedical research
por: Souren, Nicole Y, et al.
Publicado: (2022)

Cell lines authentication and mycoplasma detection as minimun quality control of cell lines in biobanking
por: Corral-Vázquez, C., et al.
Publicado: (2017)

Authenticity and Typicity of Traditional Cheeses: A Review on Geographical Origin Authentication Methods
por: Cardin, Marco, et al.
Publicado: (2022)

Interrupted coding sequences in Mycobacterium smegmatis: authentic mutations or sequencing errors?
por: Deshayes, Caroline, et al.
Publicado: (2007)

Toward on-site food authentication using nanopore sequencing
por: Voorhuijzen-Harink, Marleen M., et al.
Publicado: (2019)

Authentication of Herbal Supplements Using Next-Generation Sequencing
por: Ivanova, Natalia V., et al.
Publicado: (2016)

Determining the Authenticity of Shark Meat Products by DNA Sequencing
por: Marchetti, Patrizia, et al.
Publicado: (2020)

Evaluating the usefulness of next-generation sequencing for herb authentication
por: Delgado-Tejedor, Anna, et al.
Publicado: (2021)

Kinase Suppressor of RAS 1 (KSR1) Maintains the Transformed Phenotype of BRAFV600E Mutant Human Melanoma Cells
por: Liu, Zhi, et al.
Publicado: (2023)

Cannot write session to /tmp/vufind_sessions/sess_dp5q46n72rnnqjl1adbipmdhrt