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Community- and hospital-acquired infections with oseltamivir- and peramivir-resistant influenza A(H1N1)pdm09 viruses during the 2015–2016 season in Japan

We report five cases of community- and hospital-acquired infections with oseltamivir- and peramivir-resistant A(H1N1)pdm09 viruses possessing the neuraminidase (NA) H275Y mutation during January–February 2016 in Japan. One case was hospitalized and was receiving oseltamivir for prophylaxis. The rema...

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Autores principales: Hibino, Akinobu, Kondo, Hiroki, Masaki, Hironori, Tanabe, Yoshinari, Sato, Isamu, Takemae, Nobuhiro, Saito, Takehiko, Zaraket, Hassan, Saito, Reiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5306182/
https://www.ncbi.nlm.nih.gov/pubmed/27714496
http://dx.doi.org/10.1007/s11262-016-1396-9
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author Hibino, Akinobu
Kondo, Hiroki
Masaki, Hironori
Tanabe, Yoshinari
Sato, Isamu
Takemae, Nobuhiro
Saito, Takehiko
Zaraket, Hassan
Saito, Reiko
author_facet Hibino, Akinobu
Kondo, Hiroki
Masaki, Hironori
Tanabe, Yoshinari
Sato, Isamu
Takemae, Nobuhiro
Saito, Takehiko
Zaraket, Hassan
Saito, Reiko
author_sort Hibino, Akinobu
collection PubMed
description We report five cases of community- and hospital-acquired infections with oseltamivir- and peramivir-resistant A(H1N1)pdm09 viruses possessing the neuraminidase (NA) H275Y mutation during January–February 2016 in Japan. One case was hospitalized and was receiving oseltamivir for prophylaxis. The remaining four cases were not taking antiviral drugs at the time of sampling. These cases were geographically distant and epidemiologically unrelated. The five viruses showed ~300-fold rise in IC(50) values against oseltamivir and peramivir, defined as highly reduced inhibition according to the WHO definition. Overall, the prevalence of the H275Y A(H1N1)pdm09 viruses was 1.8 % (5/282). The resistant viruses possessed the V241I, N369 K, and N386 K substitutions in the NA that have been previously reported among A(H1N1)pdm09 to alter transmission fitness. Analysis of Michaelis constant (Km) revealed that two of the isolates had reduced NA affinity to MUNANA, while the other three isolates displayed a slightly decreased affinity compared to the sensitive viruses. Further studies are needed to monitor the community spread of resistant viruses and to assess their transmissibility. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s11262-016-1396-9) contains supplementary material, which is available to authorized users.
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spelling pubmed-53061822017-02-24 Community- and hospital-acquired infections with oseltamivir- and peramivir-resistant influenza A(H1N1)pdm09 viruses during the 2015–2016 season in Japan Hibino, Akinobu Kondo, Hiroki Masaki, Hironori Tanabe, Yoshinari Sato, Isamu Takemae, Nobuhiro Saito, Takehiko Zaraket, Hassan Saito, Reiko Virus Genes Article We report five cases of community- and hospital-acquired infections with oseltamivir- and peramivir-resistant A(H1N1)pdm09 viruses possessing the neuraminidase (NA) H275Y mutation during January–February 2016 in Japan. One case was hospitalized and was receiving oseltamivir for prophylaxis. The remaining four cases were not taking antiviral drugs at the time of sampling. These cases were geographically distant and epidemiologically unrelated. The five viruses showed ~300-fold rise in IC(50) values against oseltamivir and peramivir, defined as highly reduced inhibition according to the WHO definition. Overall, the prevalence of the H275Y A(H1N1)pdm09 viruses was 1.8 % (5/282). The resistant viruses possessed the V241I, N369 K, and N386 K substitutions in the NA that have been previously reported among A(H1N1)pdm09 to alter transmission fitness. Analysis of Michaelis constant (Km) revealed that two of the isolates had reduced NA affinity to MUNANA, while the other three isolates displayed a slightly decreased affinity compared to the sensitive viruses. Further studies are needed to monitor the community spread of resistant viruses and to assess their transmissibility. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s11262-016-1396-9) contains supplementary material, which is available to authorized users. Springer US 2016-10-06 2017 /pmc/articles/PMC5306182/ /pubmed/27714496 http://dx.doi.org/10.1007/s11262-016-1396-9 Text en © The Author(s) 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Article
Hibino, Akinobu
Kondo, Hiroki
Masaki, Hironori
Tanabe, Yoshinari
Sato, Isamu
Takemae, Nobuhiro
Saito, Takehiko
Zaraket, Hassan
Saito, Reiko
Community- and hospital-acquired infections with oseltamivir- and peramivir-resistant influenza A(H1N1)pdm09 viruses during the 2015–2016 season in Japan
title Community- and hospital-acquired infections with oseltamivir- and peramivir-resistant influenza A(H1N1)pdm09 viruses during the 2015–2016 season in Japan
title_full Community- and hospital-acquired infections with oseltamivir- and peramivir-resistant influenza A(H1N1)pdm09 viruses during the 2015–2016 season in Japan
title_fullStr Community- and hospital-acquired infections with oseltamivir- and peramivir-resistant influenza A(H1N1)pdm09 viruses during the 2015–2016 season in Japan
title_full_unstemmed Community- and hospital-acquired infections with oseltamivir- and peramivir-resistant influenza A(H1N1)pdm09 viruses during the 2015–2016 season in Japan
title_short Community- and hospital-acquired infections with oseltamivir- and peramivir-resistant influenza A(H1N1)pdm09 viruses during the 2015–2016 season in Japan
title_sort community- and hospital-acquired infections with oseltamivir- and peramivir-resistant influenza a(h1n1)pdm09 viruses during the 2015–2016 season in japan
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5306182/
https://www.ncbi.nlm.nih.gov/pubmed/27714496
http://dx.doi.org/10.1007/s11262-016-1396-9
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