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Community- and hospital-acquired infections with oseltamivir- and peramivir-resistant influenza A(H1N1)pdm09 viruses during the 2015–2016 season in Japan
We report five cases of community- and hospital-acquired infections with oseltamivir- and peramivir-resistant A(H1N1)pdm09 viruses possessing the neuraminidase (NA) H275Y mutation during January–February 2016 in Japan. One case was hospitalized and was receiving oseltamivir for prophylaxis. The rema...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer US
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5306182/ https://www.ncbi.nlm.nih.gov/pubmed/27714496 http://dx.doi.org/10.1007/s11262-016-1396-9 |
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author | Hibino, Akinobu Kondo, Hiroki Masaki, Hironori Tanabe, Yoshinari Sato, Isamu Takemae, Nobuhiro Saito, Takehiko Zaraket, Hassan Saito, Reiko |
author_facet | Hibino, Akinobu Kondo, Hiroki Masaki, Hironori Tanabe, Yoshinari Sato, Isamu Takemae, Nobuhiro Saito, Takehiko Zaraket, Hassan Saito, Reiko |
author_sort | Hibino, Akinobu |
collection | PubMed |
description | We report five cases of community- and hospital-acquired infections with oseltamivir- and peramivir-resistant A(H1N1)pdm09 viruses possessing the neuraminidase (NA) H275Y mutation during January–February 2016 in Japan. One case was hospitalized and was receiving oseltamivir for prophylaxis. The remaining four cases were not taking antiviral drugs at the time of sampling. These cases were geographically distant and epidemiologically unrelated. The five viruses showed ~300-fold rise in IC(50) values against oseltamivir and peramivir, defined as highly reduced inhibition according to the WHO definition. Overall, the prevalence of the H275Y A(H1N1)pdm09 viruses was 1.8 % (5/282). The resistant viruses possessed the V241I, N369 K, and N386 K substitutions in the NA that have been previously reported among A(H1N1)pdm09 to alter transmission fitness. Analysis of Michaelis constant (Km) revealed that two of the isolates had reduced NA affinity to MUNANA, while the other three isolates displayed a slightly decreased affinity compared to the sensitive viruses. Further studies are needed to monitor the community spread of resistant viruses and to assess their transmissibility. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s11262-016-1396-9) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-5306182 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Springer US |
record_format | MEDLINE/PubMed |
spelling | pubmed-53061822017-02-24 Community- and hospital-acquired infections with oseltamivir- and peramivir-resistant influenza A(H1N1)pdm09 viruses during the 2015–2016 season in Japan Hibino, Akinobu Kondo, Hiroki Masaki, Hironori Tanabe, Yoshinari Sato, Isamu Takemae, Nobuhiro Saito, Takehiko Zaraket, Hassan Saito, Reiko Virus Genes Article We report five cases of community- and hospital-acquired infections with oseltamivir- and peramivir-resistant A(H1N1)pdm09 viruses possessing the neuraminidase (NA) H275Y mutation during January–February 2016 in Japan. One case was hospitalized and was receiving oseltamivir for prophylaxis. The remaining four cases were not taking antiviral drugs at the time of sampling. These cases were geographically distant and epidemiologically unrelated. The five viruses showed ~300-fold rise in IC(50) values against oseltamivir and peramivir, defined as highly reduced inhibition according to the WHO definition. Overall, the prevalence of the H275Y A(H1N1)pdm09 viruses was 1.8 % (5/282). The resistant viruses possessed the V241I, N369 K, and N386 K substitutions in the NA that have been previously reported among A(H1N1)pdm09 to alter transmission fitness. Analysis of Michaelis constant (Km) revealed that two of the isolates had reduced NA affinity to MUNANA, while the other three isolates displayed a slightly decreased affinity compared to the sensitive viruses. Further studies are needed to monitor the community spread of resistant viruses and to assess their transmissibility. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s11262-016-1396-9) contains supplementary material, which is available to authorized users. Springer US 2016-10-06 2017 /pmc/articles/PMC5306182/ /pubmed/27714496 http://dx.doi.org/10.1007/s11262-016-1396-9 Text en © The Author(s) 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Article Hibino, Akinobu Kondo, Hiroki Masaki, Hironori Tanabe, Yoshinari Sato, Isamu Takemae, Nobuhiro Saito, Takehiko Zaraket, Hassan Saito, Reiko Community- and hospital-acquired infections with oseltamivir- and peramivir-resistant influenza A(H1N1)pdm09 viruses during the 2015–2016 season in Japan |
title | Community- and hospital-acquired infections with oseltamivir- and peramivir-resistant influenza A(H1N1)pdm09 viruses during the 2015–2016 season in Japan |
title_full | Community- and hospital-acquired infections with oseltamivir- and peramivir-resistant influenza A(H1N1)pdm09 viruses during the 2015–2016 season in Japan |
title_fullStr | Community- and hospital-acquired infections with oseltamivir- and peramivir-resistant influenza A(H1N1)pdm09 viruses during the 2015–2016 season in Japan |
title_full_unstemmed | Community- and hospital-acquired infections with oseltamivir- and peramivir-resistant influenza A(H1N1)pdm09 viruses during the 2015–2016 season in Japan |
title_short | Community- and hospital-acquired infections with oseltamivir- and peramivir-resistant influenza A(H1N1)pdm09 viruses during the 2015–2016 season in Japan |
title_sort | community- and hospital-acquired infections with oseltamivir- and peramivir-resistant influenza a(h1n1)pdm09 viruses during the 2015–2016 season in japan |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5306182/ https://www.ncbi.nlm.nih.gov/pubmed/27714496 http://dx.doi.org/10.1007/s11262-016-1396-9 |
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