Characterization of a recombinant Newcastle disease virus expressing the glycoprotein of bovine ephemeral fever virus

Bovine ephemeral fever (BEF) is caused by the arthropod-borne bovine ephemeral fever virus (BEFV), which is a member of the family Rhabdoviridae and the genus Ephemerovirus. BEFV causes an acute febrile infection in cattle and water buffalo. In this study, a recombinant Newcastle disease virus (NDV)...

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Autores principales: Zhang, Minmin, Ge, Jinying, Wen, Zhiyuan, Chen, Weiye, Wang, Xijun, Liu, Renqiang, Bu, Zhigao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Vienna 2016
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5306239/
https://www.ncbi.nlm.nih.gov/pubmed/27757685
http://dx.doi.org/10.1007/s00705-016-3078-2
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author Zhang, Minmin
Ge, Jinying
Wen, Zhiyuan
Chen, Weiye
Wang, Xijun
Liu, Renqiang
Bu, Zhigao
author_facet Zhang, Minmin
Ge, Jinying
Wen, Zhiyuan
Chen, Weiye
Wang, Xijun
Liu, Renqiang
Bu, Zhigao
author_sort Zhang, Minmin
collection PubMed
description Bovine ephemeral fever (BEF) is caused by the arthropod-borne bovine ephemeral fever virus (BEFV), which is a member of the family Rhabdoviridae and the genus Ephemerovirus. BEFV causes an acute febrile infection in cattle and water buffalo. In this study, a recombinant Newcastle disease virus (NDV) expressing the glycoprotein (G) of BEFV (rL-BEFV-G) was constructed, and its biological characteristics in vitro and in vivo, pathogenicity, and immune response in mice and cattle were evaluated. BEFV G enabled NDV to spread from cell to cell. rL-BEFV-G remained nonvirulent in poultry and mice compared with vector LaSota virus. rL-BEFV-G triggered a high titer of neutralizing antibodies against BEFV in mice and cattle. These results suggest that rL-BEFV-G might be a suitable candidate vaccine against BEF.
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spelling pubmed-53062392017-02-24 Characterization of a recombinant Newcastle disease virus expressing the glycoprotein of bovine ephemeral fever virus Zhang, Minmin Ge, Jinying Wen, Zhiyuan Chen, Weiye Wang, Xijun Liu, Renqiang Bu, Zhigao Arch Virol Original Article Bovine ephemeral fever (BEF) is caused by the arthropod-borne bovine ephemeral fever virus (BEFV), which is a member of the family Rhabdoviridae and the genus Ephemerovirus. BEFV causes an acute febrile infection in cattle and water buffalo. In this study, a recombinant Newcastle disease virus (NDV) expressing the glycoprotein (G) of BEFV (rL-BEFV-G) was constructed, and its biological characteristics in vitro and in vivo, pathogenicity, and immune response in mice and cattle were evaluated. BEFV G enabled NDV to spread from cell to cell. rL-BEFV-G remained nonvirulent in poultry and mice compared with vector LaSota virus. rL-BEFV-G triggered a high titer of neutralizing antibodies against BEFV in mice and cattle. These results suggest that rL-BEFV-G might be a suitable candidate vaccine against BEF. Springer Vienna 2016-10-18 2017 /pmc/articles/PMC5306239/ /pubmed/27757685 http://dx.doi.org/10.1007/s00705-016-3078-2 Text en © The Author(s) 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Original Article
Zhang, Minmin
Ge, Jinying
Wen, Zhiyuan
Chen, Weiye
Wang, Xijun
Liu, Renqiang
Bu, Zhigao
Characterization of a recombinant Newcastle disease virus expressing the glycoprotein of bovine ephemeral fever virus
title Characterization of a recombinant Newcastle disease virus expressing the glycoprotein of bovine ephemeral fever virus
title_full Characterization of a recombinant Newcastle disease virus expressing the glycoprotein of bovine ephemeral fever virus
title_fullStr Characterization of a recombinant Newcastle disease virus expressing the glycoprotein of bovine ephemeral fever virus
title_full_unstemmed Characterization of a recombinant Newcastle disease virus expressing the glycoprotein of bovine ephemeral fever virus
title_short Characterization of a recombinant Newcastle disease virus expressing the glycoprotein of bovine ephemeral fever virus
title_sort characterization of a recombinant newcastle disease virus expressing the glycoprotein of bovine ephemeral fever virus
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5306239/
https://www.ncbi.nlm.nih.gov/pubmed/27757685
http://dx.doi.org/10.1007/s00705-016-3078-2
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