Harvesting pre-polarized macrophages using thermo-responsive substrates

In the cell culture environment macrophages are highly adherent cells. Currently used methods to harvest macrophages have the disadvantage of reducing cell viability and their ability to re-attach after seeding. Although thermo-responsive surfaces have been employed to harvest cell sheets no reports...

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Detalles Bibliográficos
Autores principales: Malheiro, Vera, Elbs-Glatz, Yvonne, Obarzanek-Fojt, Magdalena, Maniura-Weber, Katharina, Bruinink, Arie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5307341/
https://www.ncbi.nlm.nih.gov/pubmed/28195152
http://dx.doi.org/10.1038/srep42495
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author Malheiro, Vera
Elbs-Glatz, Yvonne
Obarzanek-Fojt, Magdalena
Maniura-Weber, Katharina
Bruinink, Arie
author_facet Malheiro, Vera
Elbs-Glatz, Yvonne
Obarzanek-Fojt, Magdalena
Maniura-Weber, Katharina
Bruinink, Arie
author_sort Malheiro, Vera
collection PubMed
description In the cell culture environment macrophages are highly adherent cells. Currently used methods to harvest macrophages have the disadvantage of reducing cell viability and their ability to re-attach after seeding. Although thermo-responsive surfaces have been employed to harvest cell sheets no reports are available to use these to harvest (pre-polarized) macrophages. We show that this method significantly improves the yield of living macrophages and percentage of subsequent cell reattachment, whilst having a minimal effect on the cell phenotype.
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spelling pubmed-53073412017-02-22 Harvesting pre-polarized macrophages using thermo-responsive substrates Malheiro, Vera Elbs-Glatz, Yvonne Obarzanek-Fojt, Magdalena Maniura-Weber, Katharina Bruinink, Arie Sci Rep Article In the cell culture environment macrophages are highly adherent cells. Currently used methods to harvest macrophages have the disadvantage of reducing cell viability and their ability to re-attach after seeding. Although thermo-responsive surfaces have been employed to harvest cell sheets no reports are available to use these to harvest (pre-polarized) macrophages. We show that this method significantly improves the yield of living macrophages and percentage of subsequent cell reattachment, whilst having a minimal effect on the cell phenotype. Nature Publishing Group 2017-02-14 /pmc/articles/PMC5307341/ /pubmed/28195152 http://dx.doi.org/10.1038/srep42495 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Malheiro, Vera
Elbs-Glatz, Yvonne
Obarzanek-Fojt, Magdalena
Maniura-Weber, Katharina
Bruinink, Arie
Harvesting pre-polarized macrophages using thermo-responsive substrates
title Harvesting pre-polarized macrophages using thermo-responsive substrates
title_full Harvesting pre-polarized macrophages using thermo-responsive substrates
title_fullStr Harvesting pre-polarized macrophages using thermo-responsive substrates
title_full_unstemmed Harvesting pre-polarized macrophages using thermo-responsive substrates
title_short Harvesting pre-polarized macrophages using thermo-responsive substrates
title_sort harvesting pre-polarized macrophages using thermo-responsive substrates
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5307341/
https://www.ncbi.nlm.nih.gov/pubmed/28195152
http://dx.doi.org/10.1038/srep42495
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