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Induction of Invertebrate Larval Settlement; Different Bacteria, Different Mechanisms?
Recruitment via settlement of pelagic larvae is critical for the persistence of benthic marine populations. For many benthic invertebrates, larval settlement occurs in response to surface microbial films. Larvae of the serpulid polychaete Hydroides elegans can be induced to settle by single bacteria...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5307369/ https://www.ncbi.nlm.nih.gov/pubmed/28195220 http://dx.doi.org/10.1038/srep42557 |
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author | Freckelton, Marnie L. Nedved, Brian T. Hadfield, Michael G. |
author_facet | Freckelton, Marnie L. Nedved, Brian T. Hadfield, Michael G. |
author_sort | Freckelton, Marnie L. |
collection | PubMed |
description | Recruitment via settlement of pelagic larvae is critical for the persistence of benthic marine populations. For many benthic invertebrates, larval settlement occurs in response to surface microbial films. Larvae of the serpulid polychaete Hydroides elegans can be induced to settle by single bacterial species. Until now, only Pseudoalteromonas luteoviolacea had been subjected to detailed genetic and mechanistic studies. To determine if the complex structures, termed tailocins, derived from phage-tail gene assemblies and hypothesized to be the settlement cue in P. luteoviolacea were present in all inductive bacteria, genomic comparisons with inductive strains of Cellulophaga lytica, Bacillus aquimaris and Staphylococcus warneri were undertaken. They revealed that the gene assemblies for tailocins are lacking in these other bacteria. Negatively stained TEM images confirmed the absence of tailocins and revealed instead large numbers of extracellular vesicles in settlement-inductive fractions from all three bacteria. TEM imaging confirmed for C. lytica that the vesicles are budded from cell surfaces in a manner consistent with the production of outer membrane vesicles. Finding multiple bacteria settlement cues highlights the importance of further studies into the role of bacterial extracellular vesicles in eliciting settlement and metamorphosis of benthic marine larvae. |
format | Online Article Text |
id | pubmed-5307369 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-53073692017-02-22 Induction of Invertebrate Larval Settlement; Different Bacteria, Different Mechanisms? Freckelton, Marnie L. Nedved, Brian T. Hadfield, Michael G. Sci Rep Article Recruitment via settlement of pelagic larvae is critical for the persistence of benthic marine populations. For many benthic invertebrates, larval settlement occurs in response to surface microbial films. Larvae of the serpulid polychaete Hydroides elegans can be induced to settle by single bacterial species. Until now, only Pseudoalteromonas luteoviolacea had been subjected to detailed genetic and mechanistic studies. To determine if the complex structures, termed tailocins, derived from phage-tail gene assemblies and hypothesized to be the settlement cue in P. luteoviolacea were present in all inductive bacteria, genomic comparisons with inductive strains of Cellulophaga lytica, Bacillus aquimaris and Staphylococcus warneri were undertaken. They revealed that the gene assemblies for tailocins are lacking in these other bacteria. Negatively stained TEM images confirmed the absence of tailocins and revealed instead large numbers of extracellular vesicles in settlement-inductive fractions from all three bacteria. TEM imaging confirmed for C. lytica that the vesicles are budded from cell surfaces in a manner consistent with the production of outer membrane vesicles. Finding multiple bacteria settlement cues highlights the importance of further studies into the role of bacterial extracellular vesicles in eliciting settlement and metamorphosis of benthic marine larvae. Nature Publishing Group 2017-02-14 /pmc/articles/PMC5307369/ /pubmed/28195220 http://dx.doi.org/10.1038/srep42557 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Freckelton, Marnie L. Nedved, Brian T. Hadfield, Michael G. Induction of Invertebrate Larval Settlement; Different Bacteria, Different Mechanisms? |
title | Induction of Invertebrate Larval Settlement; Different Bacteria, Different Mechanisms? |
title_full | Induction of Invertebrate Larval Settlement; Different Bacteria, Different Mechanisms? |
title_fullStr | Induction of Invertebrate Larval Settlement; Different Bacteria, Different Mechanisms? |
title_full_unstemmed | Induction of Invertebrate Larval Settlement; Different Bacteria, Different Mechanisms? |
title_short | Induction of Invertebrate Larval Settlement; Different Bacteria, Different Mechanisms? |
title_sort | induction of invertebrate larval settlement; different bacteria, different mechanisms? |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5307369/ https://www.ncbi.nlm.nih.gov/pubmed/28195220 http://dx.doi.org/10.1038/srep42557 |
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