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Therapeutic Effect of Dendrobium candidum on Lupus Nephritis in Mice
CONTEXT: Dendrobium candidum (D. candimum) widely is a functional drug. The curative effect of D. candidum on lupus nephritis has been studied in vivo. MATERIALS AND METHOD: The DBA/2 and B6D2F1 mice were used for this in vivo experiment. The 50% effective dose (ED(50)) was used to check the effecti...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Medknow Publications & Media Pvt Ltd
2017
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5307896/ https://www.ncbi.nlm.nih.gov/pubmed/28216896 http://dx.doi.org/10.4103/0973-1296.197653 |
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author | Wang, Qiang Sun, Peng Wang, Rui Zhao, Xin |
author_facet | Wang, Qiang Sun, Peng Wang, Rui Zhao, Xin |
author_sort | Wang, Qiang |
collection | PubMed |
description | CONTEXT: Dendrobium candidum (D. candimum) widely is a functional drug. The curative effect of D. candidum on lupus nephritis has been studied in vivo. MATERIALS AND METHOD: The DBA/2 and B6D2F1 mice were used for this in vivo experiment. The 50% effective dose (ED(50)) was used to check the effective concentration for this study. Then the SCr, BUN, TC, TG, IL-6, IL-12, TNF-α, and IFN-γ levels were determined by kits. The output of urine protein was determined by means of Coomassie Brilliant Blue, and the auto-antibody dsDNA was determined with titer plate technology and indirect immunofluorescence. The NF-κB, IκB-α, TGF ‘β1, Fas, and FasL expressions were measured by RT-PCR and western blot assay. The component analysis of D. candidum was determined by nuclear magnetic resonance. RESULTS: Based on the ED(50) result at 329 mg/kg, 200 and 400 mg/kg doses were chosen for this study. SCr, BUN, TC and TG levels of 400 mg/kg D. candidum mice were lower than control mice, TP and ALB levels were higher than control mice. The control and 400 mg/kg treated mice tested positive for dsDNA at the end of sixth and tenth week after the experiment began. The glomerular number of 400 mg/kg treated mice was more than control group. Treatment with 400 mg/kg D. candidum reduced IL-6, IL-12, TNF-α and IFN-γcytokine levels as compared to control mice. D. candidum decreased NF-κb, TGF ‘β1, Fas, FasL and increased IκB-α expressions in kidney tissue. There were 11 compounds in dry D. candidum, these compounds might make the curative effects of lupus nephritis. CONCLUSION: D. candidum showed a potential curative effect on lupus nephritis. It could be used as a health medicine on lupus nephritis. SUMMARY: D. candidum reduced the SCr, BUN, TC, TG serum levels and raised the TP, ALB levels compared to control group. The glomerular number of D. candidum treated mice was more than control group. D. candidum treated mice showed lower IL-6, IL-12, TNF-α and IFN-γ cytokine levels than control mice. D. candidum decreased NF-κb, TGF-β1, Fas, FasL and increased IκB-α expressions in kidney tissue. Abbreviations used: LN: Lupus nephritis, SLE: systemic lupus erythematosus, D. candidum: Dendrobium candidum; IL-6: interleukin-6, IL-12: interleukin-12, TNF-α: tumor necrosis factor alpha, IFN-γ: Interferon-gamma, SCr: serum creatinine, BUN: blood urea nitrogen, TC: total cholesterol, TG: triglyceride, TP: total protein, ALB: albumin. |
format | Online Article Text |
id | pubmed-5307896 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Medknow Publications & Media Pvt Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-53078962017-02-17 Therapeutic Effect of Dendrobium candidum on Lupus Nephritis in Mice Wang, Qiang Sun, Peng Wang, Rui Zhao, Xin Pharmacogn Mag Original Article CONTEXT: Dendrobium candidum (D. candimum) widely is a functional drug. The curative effect of D. candidum on lupus nephritis has been studied in vivo. MATERIALS AND METHOD: The DBA/2 and B6D2F1 mice were used for this in vivo experiment. The 50% effective dose (ED(50)) was used to check the effective concentration for this study. Then the SCr, BUN, TC, TG, IL-6, IL-12, TNF-α, and IFN-γ levels were determined by kits. The output of urine protein was determined by means of Coomassie Brilliant Blue, and the auto-antibody dsDNA was determined with titer plate technology and indirect immunofluorescence. The NF-κB, IκB-α, TGF ‘β1, Fas, and FasL expressions were measured by RT-PCR and western blot assay. The component analysis of D. candidum was determined by nuclear magnetic resonance. RESULTS: Based on the ED(50) result at 329 mg/kg, 200 and 400 mg/kg doses were chosen for this study. SCr, BUN, TC and TG levels of 400 mg/kg D. candidum mice were lower than control mice, TP and ALB levels were higher than control mice. The control and 400 mg/kg treated mice tested positive for dsDNA at the end of sixth and tenth week after the experiment began. The glomerular number of 400 mg/kg treated mice was more than control group. Treatment with 400 mg/kg D. candidum reduced IL-6, IL-12, TNF-α and IFN-γcytokine levels as compared to control mice. D. candidum decreased NF-κb, TGF ‘β1, Fas, FasL and increased IκB-α expressions in kidney tissue. There were 11 compounds in dry D. candidum, these compounds might make the curative effects of lupus nephritis. CONCLUSION: D. candidum showed a potential curative effect on lupus nephritis. It could be used as a health medicine on lupus nephritis. SUMMARY: D. candidum reduced the SCr, BUN, TC, TG serum levels and raised the TP, ALB levels compared to control group. The glomerular number of D. candidum treated mice was more than control group. D. candidum treated mice showed lower IL-6, IL-12, TNF-α and IFN-γ cytokine levels than control mice. D. candidum decreased NF-κb, TGF-β1, Fas, FasL and increased IκB-α expressions in kidney tissue. Abbreviations used: LN: Lupus nephritis, SLE: systemic lupus erythematosus, D. candidum: Dendrobium candidum; IL-6: interleukin-6, IL-12: interleukin-12, TNF-α: tumor necrosis factor alpha, IFN-γ: Interferon-gamma, SCr: serum creatinine, BUN: blood urea nitrogen, TC: total cholesterol, TG: triglyceride, TP: total protein, ALB: albumin. Medknow Publications & Media Pvt Ltd 2017 /pmc/articles/PMC5307896/ /pubmed/28216896 http://dx.doi.org/10.4103/0973-1296.197653 Text en Copyright: © 2017 Pharmacognosy Magazine http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms. |
spellingShingle | Original Article Wang, Qiang Sun, Peng Wang, Rui Zhao, Xin Therapeutic Effect of Dendrobium candidum on Lupus Nephritis in Mice |
title | Therapeutic Effect of Dendrobium candidum on Lupus Nephritis in Mice |
title_full | Therapeutic Effect of Dendrobium candidum on Lupus Nephritis in Mice |
title_fullStr | Therapeutic Effect of Dendrobium candidum on Lupus Nephritis in Mice |
title_full_unstemmed | Therapeutic Effect of Dendrobium candidum on Lupus Nephritis in Mice |
title_short | Therapeutic Effect of Dendrobium candidum on Lupus Nephritis in Mice |
title_sort | therapeutic effect of dendrobium candidum on lupus nephritis in mice |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5307896/ https://www.ncbi.nlm.nih.gov/pubmed/28216896 http://dx.doi.org/10.4103/0973-1296.197653 |
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