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Effect of Absolute From Hibiscus syriacus L. Flower on Wound Healing in Keratinocytes

BACKGROUND: Proliferation and migration of keratinocytes are essential for the repair of cutaneous wounds. Hibiscus syriacus L. has been used in Asian medicine; however, research on keratinocytes is inadequate. OBJECTIVE: To establish the dermatological properties of absolute from Hibiscus syriacus...

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Autores principales: Yoon, Seok Won, Lee, Kang Pa, Kim, Do-Yoon, Hwang, Dae Il, Won, Kyung-Jong, Lee, Dae Won, Lee, Hwan Myung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5307920/
https://www.ncbi.nlm.nih.gov/pubmed/28216888
http://dx.doi.org/10.4103/0973-1296.197644
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author Yoon, Seok Won
Lee, Kang Pa
Kim, Do-Yoon
Hwang, Dae Il
Won, Kyung-Jong
Lee, Dae Won
Lee, Hwan Myung
author_facet Yoon, Seok Won
Lee, Kang Pa
Kim, Do-Yoon
Hwang, Dae Il
Won, Kyung-Jong
Lee, Dae Won
Lee, Hwan Myung
author_sort Yoon, Seok Won
collection PubMed
description BACKGROUND: Proliferation and migration of keratinocytes are essential for the repair of cutaneous wounds. Hibiscus syriacus L. has been used in Asian medicine; however, research on keratinocytes is inadequate. OBJECTIVE: To establish the dermatological properties of absolute from Hibiscus syriacus L. flower (HSF) and to provide fundamental research for alternative medicine. MATERIALS AND METHODS: We identified the composition of HSF absolute using gas chromatography-mass spectrometry analysis. We also examined the effect of HSF absolute in HaCaT cells using the XTT assay, Boyden chamber assay, sprout-out growth assay, and western blotting. We conducted an in-vivo wound healing assay in rat tail-skin. RESULTS: Ten major active compounds were identified from HSF absolute. As determined by the XTT assay, Boyden chamber assay, and sprout-out growth assay results, HSF absolute exhibited similar effects as that of epidermal growth factor on the proliferation and migration patterns of keratinocytes (HaCaT cells), which were significantly increased after HSF absolute treatment. The expression levels of the phosphorylated signaling proteins relevant to proliferation, including extracellular signal-regulated kinase 1/2 (Erk 1/2) and Akt, were also determined by western blot analysis. CONCLUSION: These results of our in-vitro and ex-vivo studies indicate that HSF absolute induced cell growth and migration of HaCaT cells by phosphorylating both Erk 1/2 and Akt. Moreover, we confirmed the wound-healing effect of HSF on injury of the rat tail-skin. Therefore, our results suggest that HSF absolute is promising for use in cosmetics and alternative medicine. SUMMARY: Hisbiscus syriacus L. flower absolute increases HaCaT cell migration and proliferation. Hisbiscus syriacus L. flower absolute regulates phosphorylation of ERK 1/2 and Akt in HaCaT cell. Treatment with Hisbiscus syriacus L. flower induced sprout outgrowth. The wound in the tail-skin of rat was reduced by Hisbiscus syriacus L. flower absolute. Abbreviations used: HSF: Hibiscus syriacus L. flower, Erk 1/2: extracellular signal-regulated kinase 1/2, EGF: epidermal growth factor, GC/MS: gas chromatography-mass spectrometry, DMEM: dulbecco's modified eagle medium, FBS: fetal bovine serum, BSA: bovine serum albumin, p-Akt: phosphorylation of Akt, p-Erk 1/2: phosphorylation of Erk 1/2.
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spelling pubmed-53079202017-02-17 Effect of Absolute From Hibiscus syriacus L. Flower on Wound Healing in Keratinocytes Yoon, Seok Won Lee, Kang Pa Kim, Do-Yoon Hwang, Dae Il Won, Kyung-Jong Lee, Dae Won Lee, Hwan Myung Pharmacogn Mag Original Article BACKGROUND: Proliferation and migration of keratinocytes are essential for the repair of cutaneous wounds. Hibiscus syriacus L. has been used in Asian medicine; however, research on keratinocytes is inadequate. OBJECTIVE: To establish the dermatological properties of absolute from Hibiscus syriacus L. flower (HSF) and to provide fundamental research for alternative medicine. MATERIALS AND METHODS: We identified the composition of HSF absolute using gas chromatography-mass spectrometry analysis. We also examined the effect of HSF absolute in HaCaT cells using the XTT assay, Boyden chamber assay, sprout-out growth assay, and western blotting. We conducted an in-vivo wound healing assay in rat tail-skin. RESULTS: Ten major active compounds were identified from HSF absolute. As determined by the XTT assay, Boyden chamber assay, and sprout-out growth assay results, HSF absolute exhibited similar effects as that of epidermal growth factor on the proliferation and migration patterns of keratinocytes (HaCaT cells), which were significantly increased after HSF absolute treatment. The expression levels of the phosphorylated signaling proteins relevant to proliferation, including extracellular signal-regulated kinase 1/2 (Erk 1/2) and Akt, were also determined by western blot analysis. CONCLUSION: These results of our in-vitro and ex-vivo studies indicate that HSF absolute induced cell growth and migration of HaCaT cells by phosphorylating both Erk 1/2 and Akt. Moreover, we confirmed the wound-healing effect of HSF on injury of the rat tail-skin. Therefore, our results suggest that HSF absolute is promising for use in cosmetics and alternative medicine. SUMMARY: Hisbiscus syriacus L. flower absolute increases HaCaT cell migration and proliferation. Hisbiscus syriacus L. flower absolute regulates phosphorylation of ERK 1/2 and Akt in HaCaT cell. Treatment with Hisbiscus syriacus L. flower induced sprout outgrowth. The wound in the tail-skin of rat was reduced by Hisbiscus syriacus L. flower absolute. Abbreviations used: HSF: Hibiscus syriacus L. flower, Erk 1/2: extracellular signal-regulated kinase 1/2, EGF: epidermal growth factor, GC/MS: gas chromatography-mass spectrometry, DMEM: dulbecco's modified eagle medium, FBS: fetal bovine serum, BSA: bovine serum albumin, p-Akt: phosphorylation of Akt, p-Erk 1/2: phosphorylation of Erk 1/2. Medknow Publications & Media Pvt Ltd 2017 /pmc/articles/PMC5307920/ /pubmed/28216888 http://dx.doi.org/10.4103/0973-1296.197644 Text en Copyright: © 2017 Pharmacognosy Magazine http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.
spellingShingle Original Article
Yoon, Seok Won
Lee, Kang Pa
Kim, Do-Yoon
Hwang, Dae Il
Won, Kyung-Jong
Lee, Dae Won
Lee, Hwan Myung
Effect of Absolute From Hibiscus syriacus L. Flower on Wound Healing in Keratinocytes
title Effect of Absolute From Hibiscus syriacus L. Flower on Wound Healing in Keratinocytes
title_full Effect of Absolute From Hibiscus syriacus L. Flower on Wound Healing in Keratinocytes
title_fullStr Effect of Absolute From Hibiscus syriacus L. Flower on Wound Healing in Keratinocytes
title_full_unstemmed Effect of Absolute From Hibiscus syriacus L. Flower on Wound Healing in Keratinocytes
title_short Effect of Absolute From Hibiscus syriacus L. Flower on Wound Healing in Keratinocytes
title_sort effect of absolute from hibiscus syriacus l. flower on wound healing in keratinocytes
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5307920/
https://www.ncbi.nlm.nih.gov/pubmed/28216888
http://dx.doi.org/10.4103/0973-1296.197644
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