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Osteoblasts Proliferation and Differentiation Stimulating Activities of the Main Components of Epimedii folium

BACKGROUND: Osteoporosis is a disease of bones that leads to an increased risk of fracture. Epimedii Folium is commonly used for treating bone fractures and joint diseases for thousands of years in China. METHODS: This study was aimed to screen active components, which might have the potency to stim...

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Autores principales: Liu, Mingming, Xu, Haiyan, Ma, Yong, Cheng, Jian, Hua, Zhen, Huang, Guicheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5307921/
https://www.ncbi.nlm.nih.gov/pubmed/28216889
http://dx.doi.org/10.4103/0973-1296.197654
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author Liu, Mingming
Xu, Haiyan
Ma, Yong
Cheng, Jian
Hua, Zhen
Huang, Guicheng
author_facet Liu, Mingming
Xu, Haiyan
Ma, Yong
Cheng, Jian
Hua, Zhen
Huang, Guicheng
author_sort Liu, Mingming
collection PubMed
description BACKGROUND: Osteoporosis is a disease of bones that leads to an increased risk of fracture. Epimedii Folium is commonly used for treating bone fractures and joint diseases for thousands of years in China. METHODS: This study was aimed to screen active components, which might have the potency to stimulate osteoblasts proliferation and differentiation in Epimedii Folium. An HPLC method was established to analyze the main components in Epimedii Folium. The MTT and ALP methods were utilized for the assay of osteoblasts proliferation and differentiation activity. Bavachin, a flavonoid compound was treated as the positive control. RESULTS: Totally eight compounds have been identified by comparing their retention time with correspondent standard substances. Icariside I and icariside II significantly stimulated cell proliferation and osteoblasts differentiation. All these compounds were found with a characterized flavonoid structure in each of their molecule backbones. CONCLUSION: These results lead to a hypothesis that flavonoid monoglycoside structure might be crucial to exhibit the activity. The structure–effect relationship of these compounds with flavonoid monoglycoside structure in mouse primary calvarial osteoblasts needs to be explored in further research. SUMMARY: Eight compounds were identified by comparing their retention time with correspondent standard substances. Icariside I and icariside II significantly stimulated cell proliferation and osteoblasts differentiation. Flavonoid monoglycoside structure might be crucial to exhibit the osteoblasts proliferation and differentiation activity. Effects of the main components of Epimedii Folium on osteoblasts proliferation after treating 48 h. Abbreviations used: HPLC: High performance liquid chromatography, MTT: Methylthiazolyldiphenyl - tetrazolium bromide, ALP: Alkaline phosphatase
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spelling pubmed-53079212017-02-17 Osteoblasts Proliferation and Differentiation Stimulating Activities of the Main Components of Epimedii folium Liu, Mingming Xu, Haiyan Ma, Yong Cheng, Jian Hua, Zhen Huang, Guicheng Pharmacogn Mag Original Article BACKGROUND: Osteoporosis is a disease of bones that leads to an increased risk of fracture. Epimedii Folium is commonly used for treating bone fractures and joint diseases for thousands of years in China. METHODS: This study was aimed to screen active components, which might have the potency to stimulate osteoblasts proliferation and differentiation in Epimedii Folium. An HPLC method was established to analyze the main components in Epimedii Folium. The MTT and ALP methods were utilized for the assay of osteoblasts proliferation and differentiation activity. Bavachin, a flavonoid compound was treated as the positive control. RESULTS: Totally eight compounds have been identified by comparing their retention time with correspondent standard substances. Icariside I and icariside II significantly stimulated cell proliferation and osteoblasts differentiation. All these compounds were found with a characterized flavonoid structure in each of their molecule backbones. CONCLUSION: These results lead to a hypothesis that flavonoid monoglycoside structure might be crucial to exhibit the activity. The structure–effect relationship of these compounds with flavonoid monoglycoside structure in mouse primary calvarial osteoblasts needs to be explored in further research. SUMMARY: Eight compounds were identified by comparing their retention time with correspondent standard substances. Icariside I and icariside II significantly stimulated cell proliferation and osteoblasts differentiation. Flavonoid monoglycoside structure might be crucial to exhibit the osteoblasts proliferation and differentiation activity. Effects of the main components of Epimedii Folium on osteoblasts proliferation after treating 48 h. Abbreviations used: HPLC: High performance liquid chromatography, MTT: Methylthiazolyldiphenyl - tetrazolium bromide, ALP: Alkaline phosphatase Medknow Publications & Media Pvt Ltd 2017 /pmc/articles/PMC5307921/ /pubmed/28216889 http://dx.doi.org/10.4103/0973-1296.197654 Text en Copyright: © 2017 Pharmacognosy Magazine http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.
spellingShingle Original Article
Liu, Mingming
Xu, Haiyan
Ma, Yong
Cheng, Jian
Hua, Zhen
Huang, Guicheng
Osteoblasts Proliferation and Differentiation Stimulating Activities of the Main Components of Epimedii folium
title Osteoblasts Proliferation and Differentiation Stimulating Activities of the Main Components of Epimedii folium
title_full Osteoblasts Proliferation and Differentiation Stimulating Activities of the Main Components of Epimedii folium
title_fullStr Osteoblasts Proliferation and Differentiation Stimulating Activities of the Main Components of Epimedii folium
title_full_unstemmed Osteoblasts Proliferation and Differentiation Stimulating Activities of the Main Components of Epimedii folium
title_short Osteoblasts Proliferation and Differentiation Stimulating Activities of the Main Components of Epimedii folium
title_sort osteoblasts proliferation and differentiation stimulating activities of the main components of epimedii folium
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5307921/
https://www.ncbi.nlm.nih.gov/pubmed/28216889
http://dx.doi.org/10.4103/0973-1296.197654
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