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CacyBP/SIP promotes the proliferation of colon cancer cells
CacyBP/SIP is a component of the ubiquitin pathway and is overexpressed in several transformed tumor tissues, including colon cancer, which is one of the most common cancers worldwide. It is unknown whether CacyBP/SIP promotes the proliferation of colon cancer cells. This study examined the expressi...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5308830/ https://www.ncbi.nlm.nih.gov/pubmed/28196083 http://dx.doi.org/10.1371/journal.pone.0169959 |
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author | Zhai, Huihong Shi, Yongquan Chen, Xiong Wang, Jun Lu, Yuanyuan Zhang, Faming Liu, Zhengxiong Lei, Ting Fan, Daiming |
author_facet | Zhai, Huihong Shi, Yongquan Chen, Xiong Wang, Jun Lu, Yuanyuan Zhang, Faming Liu, Zhengxiong Lei, Ting Fan, Daiming |
author_sort | Zhai, Huihong |
collection | PubMed |
description | CacyBP/SIP is a component of the ubiquitin pathway and is overexpressed in several transformed tumor tissues, including colon cancer, which is one of the most common cancers worldwide. It is unknown whether CacyBP/SIP promotes the proliferation of colon cancer cells. This study examined the expression level, subcellular localization, and binding activity of CacyBP/SIP in human colon cancer cells in the presence and absence of the hormone gastrin. We found that CacyBP/SIP was expressed in a high percentage of colon cancer cells, but not in normal colonic surface epithelium. CacyBP/SIP promoted the cell proliferation of colon cancer cells under both basal and gastrin stimulated conditions as shown by knockdown studies. Gastrin stimulation triggered the translocation of CacyBP/SIP to the nucleus, and enhanced interaction between CacyBP/SIP and SKP1, a key component of ubiquitination pathway which further mediated the proteasome-dependent degradation of p27(kip1) protein. The gastrin induced reduction in p27(kip1) was prevented when cells were treated with the proteasome inhibitor MG132. These results suggest that CacyBP/SIP may be promoting growth of colon cancer cells by enhancing ubiquitin-mediated degradation of p27(kip1). |
format | Online Article Text |
id | pubmed-5308830 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-53088302017-02-28 CacyBP/SIP promotes the proliferation of colon cancer cells Zhai, Huihong Shi, Yongquan Chen, Xiong Wang, Jun Lu, Yuanyuan Zhang, Faming Liu, Zhengxiong Lei, Ting Fan, Daiming PLoS One Research Article CacyBP/SIP is a component of the ubiquitin pathway and is overexpressed in several transformed tumor tissues, including colon cancer, which is one of the most common cancers worldwide. It is unknown whether CacyBP/SIP promotes the proliferation of colon cancer cells. This study examined the expression level, subcellular localization, and binding activity of CacyBP/SIP in human colon cancer cells in the presence and absence of the hormone gastrin. We found that CacyBP/SIP was expressed in a high percentage of colon cancer cells, but not in normal colonic surface epithelium. CacyBP/SIP promoted the cell proliferation of colon cancer cells under both basal and gastrin stimulated conditions as shown by knockdown studies. Gastrin stimulation triggered the translocation of CacyBP/SIP to the nucleus, and enhanced interaction between CacyBP/SIP and SKP1, a key component of ubiquitination pathway which further mediated the proteasome-dependent degradation of p27(kip1) protein. The gastrin induced reduction in p27(kip1) was prevented when cells were treated with the proteasome inhibitor MG132. These results suggest that CacyBP/SIP may be promoting growth of colon cancer cells by enhancing ubiquitin-mediated degradation of p27(kip1). Public Library of Science 2017-02-14 /pmc/articles/PMC5308830/ /pubmed/28196083 http://dx.doi.org/10.1371/journal.pone.0169959 Text en © 2017 Zhai et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Zhai, Huihong Shi, Yongquan Chen, Xiong Wang, Jun Lu, Yuanyuan Zhang, Faming Liu, Zhengxiong Lei, Ting Fan, Daiming CacyBP/SIP promotes the proliferation of colon cancer cells |
title | CacyBP/SIP promotes the proliferation of colon cancer cells |
title_full | CacyBP/SIP promotes the proliferation of colon cancer cells |
title_fullStr | CacyBP/SIP promotes the proliferation of colon cancer cells |
title_full_unstemmed | CacyBP/SIP promotes the proliferation of colon cancer cells |
title_short | CacyBP/SIP promotes the proliferation of colon cancer cells |
title_sort | cacybp/sip promotes the proliferation of colon cancer cells |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5308830/ https://www.ncbi.nlm.nih.gov/pubmed/28196083 http://dx.doi.org/10.1371/journal.pone.0169959 |
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