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Batf3 selectively determines acquisition of CD8+ dendritic cell phenotype and function

Batf3 is a transcription factor that impacts the development of CD103+ tissue-resident dendritic cells (DCs). However, whether Batf3 is absolutely required for the development of CD8+ DCs remains controversial. Id2 is required for CD8+ DC development. Here we show that bone marrow chimeric mice with...

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Autores principales: Chandra, Janin, Kuo, Paula TY, Hahn, Anne M., Belz, Gabrielle T., Frazer, Ian H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5309136/
https://www.ncbi.nlm.nih.gov/pubmed/27897162
http://dx.doi.org/10.1038/icb.2016.83
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author Chandra, Janin
Kuo, Paula TY
Hahn, Anne M.
Belz, Gabrielle T.
Frazer, Ian H.
author_facet Chandra, Janin
Kuo, Paula TY
Hahn, Anne M.
Belz, Gabrielle T.
Frazer, Ian H.
author_sort Chandra, Janin
collection PubMed
description Batf3 is a transcription factor that impacts the development of CD103+ tissue-resident dendritic cells (DCs). However, whether Batf3 is absolutely required for the development of CD8+ DCs remains controversial. Id2 is required for CD8+ DC development. Here we show that bone marrow chimeric mice with a deletion of Id2 in the CD11c compartment lose the ability to reject a skin graft expressing a non-self protein antigen or mount a delayed hypersensitivity response. In contrast, Batf3−/− mice remained competent for skin graft rejection and delayed hypersensitivity, and retained a CD8+ DC population with markers characteristic of the CD11b+ DC lineage including CD11b, CD4 and CD172α as well as the key regulator transcription factor IRF4, but lacked IRF8 expression. CD8+ DCs in Batf3−/− mice took up and cleaved protein antigen and larger particles but were unable to phagocytose dying cells, a characteristic feature to the CD8+ DC lineage. These data clarify a requirement for CD8+ lineage DCs for inducing effectors of neo-antigen driven skin graft rejection, and improve our understanding of DC subtype commitment by demonstrating that in the absence of Batf3, CD8+ DCs can change their fate and become CD11b+ DCs.
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spelling pubmed-53091362017-05-29 Batf3 selectively determines acquisition of CD8+ dendritic cell phenotype and function Chandra, Janin Kuo, Paula TY Hahn, Anne M. Belz, Gabrielle T. Frazer, Ian H. Immunol Cell Biol Article Batf3 is a transcription factor that impacts the development of CD103+ tissue-resident dendritic cells (DCs). However, whether Batf3 is absolutely required for the development of CD8+ DCs remains controversial. Id2 is required for CD8+ DC development. Here we show that bone marrow chimeric mice with a deletion of Id2 in the CD11c compartment lose the ability to reject a skin graft expressing a non-self protein antigen or mount a delayed hypersensitivity response. In contrast, Batf3−/− mice remained competent for skin graft rejection and delayed hypersensitivity, and retained a CD8+ DC population with markers characteristic of the CD11b+ DC lineage including CD11b, CD4 and CD172α as well as the key regulator transcription factor IRF4, but lacked IRF8 expression. CD8+ DCs in Batf3−/− mice took up and cleaved protein antigen and larger particles but were unable to phagocytose dying cells, a characteristic feature to the CD8+ DC lineage. These data clarify a requirement for CD8+ lineage DCs for inducing effectors of neo-antigen driven skin graft rejection, and improve our understanding of DC subtype commitment by demonstrating that in the absence of Batf3, CD8+ DCs can change their fate and become CD11b+ DCs. 2016-11-29 2017-02 /pmc/articles/PMC5309136/ /pubmed/27897162 http://dx.doi.org/10.1038/icb.2016.83 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Chandra, Janin
Kuo, Paula TY
Hahn, Anne M.
Belz, Gabrielle T.
Frazer, Ian H.
Batf3 selectively determines acquisition of CD8+ dendritic cell phenotype and function
title Batf3 selectively determines acquisition of CD8+ dendritic cell phenotype and function
title_full Batf3 selectively determines acquisition of CD8+ dendritic cell phenotype and function
title_fullStr Batf3 selectively determines acquisition of CD8+ dendritic cell phenotype and function
title_full_unstemmed Batf3 selectively determines acquisition of CD8+ dendritic cell phenotype and function
title_short Batf3 selectively determines acquisition of CD8+ dendritic cell phenotype and function
title_sort batf3 selectively determines acquisition of cd8+ dendritic cell phenotype and function
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5309136/
https://www.ncbi.nlm.nih.gov/pubmed/27897162
http://dx.doi.org/10.1038/icb.2016.83
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