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Fabrication of Progesterone-Loaded Nanofibers for the Drug Delivery Applications in Bovine
Progesterone is a potent drug for synchronization of the estrus and ovulation cycles in bovine. At present, the estrus cycle of bovine is controlled by the insertion of progesterone-embedded silicone bands. The disadvantage of nondegradable polymer inserts is to require for disposal of these bands a...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer US
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5309186/ https://www.ncbi.nlm.nih.gov/pubmed/28228001 http://dx.doi.org/10.1186/s11671-016-1781-2 |
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author | Karuppannan, Chitra Sivaraj, Mehnath Kumar, J. Ganesh Seerangan, Rangasamy Balasubramanian, S. Gopal, Dhinakar Raj |
author_facet | Karuppannan, Chitra Sivaraj, Mehnath Kumar, J. Ganesh Seerangan, Rangasamy Balasubramanian, S. Gopal, Dhinakar Raj |
author_sort | Karuppannan, Chitra |
collection | PubMed |
description | Progesterone is a potent drug for synchronization of the estrus and ovulation cycles in bovine. At present, the estrus cycle of bovine is controlled by the insertion of progesterone-embedded silicone bands. The disadvantage of nondegradable polymer inserts is to require for disposal of these bands after their use. The study currently focuses on preparation of biodegradable progesterone-incorporated nanofiber for estrus synchronization. Three different concentrations (1.2, 1.9, and 2.5 g) of progesterone-impregnated nanofibers were fabricated using electrospinning. The spun membrane were characterized by scanning electron microscopy, X-ray diffraction, differential scanning calorimetry, thermogravimetric analysis, and Fourier transform infrared spectroscopy. Uniform surface morphology, narrow size distribution, and interaction between progesterone and zein were confirmed by SEM. FTIR spectroscopy indicated miscibility and interaction between zein and progesterone. X-ray analysis indicated that the size of zein crystallites increased with progesterone content in nanofibers. Significant differences in thermal behavior of progesterone-impregnated nanofiber were observed by DSC. Cell viability studies of progesterone-loaded nanofiber were examined using MTT assay. In vitro release experiment is to identify the suitable progesterone concentration for estrus synchronization. This study confirms that progesterone-impregnated nanofibers are an ideal vehicle for progesterone delivery for estrus synchronization of bovines. |
format | Online Article Text |
id | pubmed-5309186 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Springer US |
record_format | MEDLINE/PubMed |
spelling | pubmed-53091862017-02-28 Fabrication of Progesterone-Loaded Nanofibers for the Drug Delivery Applications in Bovine Karuppannan, Chitra Sivaraj, Mehnath Kumar, J. Ganesh Seerangan, Rangasamy Balasubramanian, S. Gopal, Dhinakar Raj Nanoscale Res Lett Nano Express Progesterone is a potent drug for synchronization of the estrus and ovulation cycles in bovine. At present, the estrus cycle of bovine is controlled by the insertion of progesterone-embedded silicone bands. The disadvantage of nondegradable polymer inserts is to require for disposal of these bands after their use. The study currently focuses on preparation of biodegradable progesterone-incorporated nanofiber for estrus synchronization. Three different concentrations (1.2, 1.9, and 2.5 g) of progesterone-impregnated nanofibers were fabricated using electrospinning. The spun membrane were characterized by scanning electron microscopy, X-ray diffraction, differential scanning calorimetry, thermogravimetric analysis, and Fourier transform infrared spectroscopy. Uniform surface morphology, narrow size distribution, and interaction between progesterone and zein were confirmed by SEM. FTIR spectroscopy indicated miscibility and interaction between zein and progesterone. X-ray analysis indicated that the size of zein crystallites increased with progesterone content in nanofibers. Significant differences in thermal behavior of progesterone-impregnated nanofiber were observed by DSC. Cell viability studies of progesterone-loaded nanofiber were examined using MTT assay. In vitro release experiment is to identify the suitable progesterone concentration for estrus synchronization. This study confirms that progesterone-impregnated nanofibers are an ideal vehicle for progesterone delivery for estrus synchronization of bovines. Springer US 2017-02-14 /pmc/articles/PMC5309186/ /pubmed/28228001 http://dx.doi.org/10.1186/s11671-016-1781-2 Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Nano Express Karuppannan, Chitra Sivaraj, Mehnath Kumar, J. Ganesh Seerangan, Rangasamy Balasubramanian, S. Gopal, Dhinakar Raj Fabrication of Progesterone-Loaded Nanofibers for the Drug Delivery Applications in Bovine |
title | Fabrication of Progesterone-Loaded Nanofibers for the Drug Delivery Applications in Bovine |
title_full | Fabrication of Progesterone-Loaded Nanofibers for the Drug Delivery Applications in Bovine |
title_fullStr | Fabrication of Progesterone-Loaded Nanofibers for the Drug Delivery Applications in Bovine |
title_full_unstemmed | Fabrication of Progesterone-Loaded Nanofibers for the Drug Delivery Applications in Bovine |
title_short | Fabrication of Progesterone-Loaded Nanofibers for the Drug Delivery Applications in Bovine |
title_sort | fabrication of progesterone-loaded nanofibers for the drug delivery applications in bovine |
topic | Nano Express |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5309186/ https://www.ncbi.nlm.nih.gov/pubmed/28228001 http://dx.doi.org/10.1186/s11671-016-1781-2 |
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