Isolation of Clostridium difficile and molecular detection of binary and A/B toxins in faeces of dogs

The aim of this study was to isolate Clostridium difficile from dogs’ faeces, and to study the frequency of its virulence genes. A total of 151 samples of dogs’ faeces were collected. The isolation of C. difficile was performed by using the bacterial culture methods followed by DNA extraction using...

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Detalles Bibliográficos
Autores principales: Ghavidel, M., Salari Sedigh, H., Razmyar, J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: School of Veterinary Medicine, University of Shiraz 2016
Materias:
Short Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5309461/
https://www.ncbi.nlm.nih.gov/pubmed/28224013
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author Ghavidel, M.
Salari Sedigh, H.
Razmyar, J.
author_facet Ghavidel, M.
Salari Sedigh, H.
Razmyar, J.
author_sort Ghavidel, M.
collection PubMed
description The aim of this study was to isolate Clostridium difficile from dogs’ faeces, and to study the frequency of its virulence genes. A total of 151 samples of dogs’ faeces were collected. The isolation of C. difficile was performed by using the bacterial culture methods followed by DNA extraction using boiling method. Multiplex PCR method was performed for identification of tcdA, tcdB, cdtA and cdtB genes and single method was carried out for detection of tcdC. Twelve samples (7.9%) were positive in bacteriological assay and based on molecular assay, 66.7% of the isolates (8 of 12 C. difficile isolated) had shown tcdA(+), tcdB(+) profile. This is the first investigation on molecular assay of C. difficile in Iran’s dog population.
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spelling pubmed-53094612017-02-21 Isolation of Clostridium difficile and molecular detection of binary and A/B toxins in faeces of dogs Ghavidel, M. Salari Sedigh, H. Razmyar, J. Iran J Vet Res Short Paper The aim of this study was to isolate Clostridium difficile from dogs’ faeces, and to study the frequency of its virulence genes. A total of 151 samples of dogs’ faeces were collected. The isolation of C. difficile was performed by using the bacterial culture methods followed by DNA extraction using boiling method. Multiplex PCR method was performed for identification of tcdA, tcdB, cdtA and cdtB genes and single method was carried out for detection of tcdC. Twelve samples (7.9%) were positive in bacteriological assay and based on molecular assay, 66.7% of the isolates (8 of 12 C. difficile isolated) had shown tcdA(+), tcdB(+) profile. This is the first investigation on molecular assay of C. difficile in Iran’s dog population. School of Veterinary Medicine, University of Shiraz 2016 /pmc/articles/PMC5309461/ /pubmed/28224013 Text en This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Paper
Ghavidel, M.
Salari Sedigh, H.
Razmyar, J.
Isolation of Clostridium difficile and molecular detection of binary and A/B toxins in faeces of dogs
title Isolation of Clostridium difficile and molecular detection of binary and A/B toxins in faeces of dogs
title_full Isolation of Clostridium difficile and molecular detection of binary and A/B toxins in faeces of dogs
title_fullStr Isolation of Clostridium difficile and molecular detection of binary and A/B toxins in faeces of dogs
title_full_unstemmed Isolation of Clostridium difficile and molecular detection of binary and A/B toxins in faeces of dogs
title_short Isolation of Clostridium difficile and molecular detection of binary and A/B toxins in faeces of dogs
title_sort isolation of clostridium difficile and molecular detection of binary and a/b toxins in faeces of dogs
topic Short Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5309461/
https://www.ncbi.nlm.nih.gov/pubmed/28224013
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