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Organic nitrogen rearranges both structure and activity of the soil-borne microbial seedbank

Use of organic amendments is a valuable strategy for crop production. However, it remains unclear how organic amendments shape both soil microbial community structure and activity, and how these changes impact nutrient mineralization rates. We evaluated the effect of various organic amendments, whic...

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Autores principales: Leite, Márcio F. A., Pan, Yao, Bloem, Jaap, Berge, Hein ten, Kuramae, Eiko E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5309777/
https://www.ncbi.nlm.nih.gov/pubmed/28198425
http://dx.doi.org/10.1038/srep42634
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author Leite, Márcio F. A.
Pan, Yao
Bloem, Jaap
Berge, Hein ten
Kuramae, Eiko E.
author_facet Leite, Márcio F. A.
Pan, Yao
Bloem, Jaap
Berge, Hein ten
Kuramae, Eiko E.
author_sort Leite, Márcio F. A.
collection PubMed
description Use of organic amendments is a valuable strategy for crop production. However, it remains unclear how organic amendments shape both soil microbial community structure and activity, and how these changes impact nutrient mineralization rates. We evaluated the effect of various organic amendments, which range in Carbon/Nitrogen (C/N) ratio and degradability, on the soil microbiome in a mesocosm study at 32, 69 and 132 days. Soil samples were collected to determine community structure (assessed by 16S and 18S rRNA gene sequences), microbial biomass (fungi and bacteria), microbial activity (leucine incorporation and active hyphal length), and carbon and nitrogen mineralization rates. We considered the microbial soil DNA as the microbial seedbank. High C/N ratio favored fungal presence, while low C/N favored dominance of bacterial populations. Our results suggest that organic amendments shape the soil microbial community structure through a feedback mechanism by which microbial activity responds to changing organic inputs and rearranges composition of the microbial seedbank. We hypothesize that the microbial seedbank composition responds to changing organic inputs according to the resistance and resilience of individual species, while changes in microbial activity may result in increases or decreases in availability of various soil nutrients that affect plant nutrient uptake.
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spelling pubmed-53097772017-02-22 Organic nitrogen rearranges both structure and activity of the soil-borne microbial seedbank Leite, Márcio F. A. Pan, Yao Bloem, Jaap Berge, Hein ten Kuramae, Eiko E. Sci Rep Article Use of organic amendments is a valuable strategy for crop production. However, it remains unclear how organic amendments shape both soil microbial community structure and activity, and how these changes impact nutrient mineralization rates. We evaluated the effect of various organic amendments, which range in Carbon/Nitrogen (C/N) ratio and degradability, on the soil microbiome in a mesocosm study at 32, 69 and 132 days. Soil samples were collected to determine community structure (assessed by 16S and 18S rRNA gene sequences), microbial biomass (fungi and bacteria), microbial activity (leucine incorporation and active hyphal length), and carbon and nitrogen mineralization rates. We considered the microbial soil DNA as the microbial seedbank. High C/N ratio favored fungal presence, while low C/N favored dominance of bacterial populations. Our results suggest that organic amendments shape the soil microbial community structure through a feedback mechanism by which microbial activity responds to changing organic inputs and rearranges composition of the microbial seedbank. We hypothesize that the microbial seedbank composition responds to changing organic inputs according to the resistance and resilience of individual species, while changes in microbial activity may result in increases or decreases in availability of various soil nutrients that affect plant nutrient uptake. Nature Publishing Group 2017-02-15 /pmc/articles/PMC5309777/ /pubmed/28198425 http://dx.doi.org/10.1038/srep42634 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Leite, Márcio F. A.
Pan, Yao
Bloem, Jaap
Berge, Hein ten
Kuramae, Eiko E.
Organic nitrogen rearranges both structure and activity of the soil-borne microbial seedbank
title Organic nitrogen rearranges both structure and activity of the soil-borne microbial seedbank
title_full Organic nitrogen rearranges both structure and activity of the soil-borne microbial seedbank
title_fullStr Organic nitrogen rearranges both structure and activity of the soil-borne microbial seedbank
title_full_unstemmed Organic nitrogen rearranges both structure and activity of the soil-borne microbial seedbank
title_short Organic nitrogen rearranges both structure and activity of the soil-borne microbial seedbank
title_sort organic nitrogen rearranges both structure and activity of the soil-borne microbial seedbank
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5309777/
https://www.ncbi.nlm.nih.gov/pubmed/28198425
http://dx.doi.org/10.1038/srep42634
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