Method for Dual Viral Vector Mediated CRISPR-Cas9 Gene Disruption in Primary Human Endothelial Cells

Human endothelial cells (ECs) are widely used to study mechanisms of angiogenesis, inflammation, and endothelial permeability. Targeted gene disruption induced by Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-CRISPR-Associated Protein 9 (Cas9) nuclease gene editing is potentiall...

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Detalles Bibliográficos
Autores principales: Gong, Haixia, Liu, Menglin, Klomp, Jeff, Merrill, Bradley J., Rehman, Jalees, Malik, Asrar B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5309830/
https://www.ncbi.nlm.nih.gov/pubmed/28198371
http://dx.doi.org/10.1038/srep42127

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5309830/
https://www.ncbi.nlm.nih.gov/pubmed/28198371
http://dx.doi.org/10.1038/srep42127

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