Cargando…

Identification of symplasmic domains in the embryo and seed of Sedum acre L. (Crassulaceae)

Our study demonstrated that symplasmic communication between Sedum acre seed compartments and the embryo proper is not uniform. The presence of plasmodesmata (PD) constitutes the structural basis for information exchange between cells, and symplasmic communication is involved in the regulation of ce...

Descripción completa

Detalles Bibliográficos
Autores principales: Wróbel-Marek, Justyna, Kurczyńska, Ewa, Płachno, Bartosz J., Kozieradzka-Kiszkurno, Małgorzata
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5310571/
https://www.ncbi.nlm.nih.gov/pubmed/27888360
http://dx.doi.org/10.1007/s00425-016-2619-y
_version_ 1782507896006770688
author Wróbel-Marek, Justyna
Kurczyńska, Ewa
Płachno, Bartosz J.
Kozieradzka-Kiszkurno, Małgorzata
author_facet Wróbel-Marek, Justyna
Kurczyńska, Ewa
Płachno, Bartosz J.
Kozieradzka-Kiszkurno, Małgorzata
author_sort Wróbel-Marek, Justyna
collection PubMed
description Our study demonstrated that symplasmic communication between Sedum acre seed compartments and the embryo proper is not uniform. The presence of plasmodesmata (PD) constitutes the structural basis for information exchange between cells, and symplasmic communication is involved in the regulation of cell differentiation and plant development. Most recent studies concerning an analysis of symplasmic communication between seed compartments and the embryo have been predominantly performed on Arabidopsis thaliana. The results presented in this paper describe the analysis of symplasmic communication on the example of Sedum acre seeds, because the ultrastructure of the seed compartments and the embryo proper, including the PD, have already been described, and this species represents an embryonic type of development different to Arabidopsis. Moreover, in this species, an unusual electron-dense dome associated with plasmodesmata on the border between the basal cell/chalazal suspensor cells and the basal cell/the endosperm has been described. This prompted the question as to whether these plasmodesmata are functional. Thus, the aim of this study was to describe the movement of symplasmic transport fluorochromes between different Sedum seed compartments, with particular emphasis on the movement between the basal cell and the embryo proper and endosperm, to answer the following questions: (1) are seeds divided into symplasmic domains; (2) if so, are they stable or do they change with the development? The results have shown that symplasmic tracers movement: (a) from the external integument to internal integument is restricted; (b) from the basal cell to the other part of the embryo proper and from the basal cell to the endosperm is also restricted; (c) the embryo is a single symplasmic domain with respect to molecules of a molecular weight below 0.5 kDa. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00425-016-2619-y) contains supplementary material, which is available to authorized users.
format Online
Article
Text
id pubmed-5310571
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Springer Berlin Heidelberg
record_format MEDLINE/PubMed
spelling pubmed-53105712017-02-28 Identification of symplasmic domains in the embryo and seed of Sedum acre L. (Crassulaceae) Wróbel-Marek, Justyna Kurczyńska, Ewa Płachno, Bartosz J. Kozieradzka-Kiszkurno, Małgorzata Planta Original Article Our study demonstrated that symplasmic communication between Sedum acre seed compartments and the embryo proper is not uniform. The presence of plasmodesmata (PD) constitutes the structural basis for information exchange between cells, and symplasmic communication is involved in the regulation of cell differentiation and plant development. Most recent studies concerning an analysis of symplasmic communication between seed compartments and the embryo have been predominantly performed on Arabidopsis thaliana. The results presented in this paper describe the analysis of symplasmic communication on the example of Sedum acre seeds, because the ultrastructure of the seed compartments and the embryo proper, including the PD, have already been described, and this species represents an embryonic type of development different to Arabidopsis. Moreover, in this species, an unusual electron-dense dome associated with plasmodesmata on the border between the basal cell/chalazal suspensor cells and the basal cell/the endosperm has been described. This prompted the question as to whether these plasmodesmata are functional. Thus, the aim of this study was to describe the movement of symplasmic transport fluorochromes between different Sedum seed compartments, with particular emphasis on the movement between the basal cell and the embryo proper and endosperm, to answer the following questions: (1) are seeds divided into symplasmic domains; (2) if so, are they stable or do they change with the development? The results have shown that symplasmic tracers movement: (a) from the external integument to internal integument is restricted; (b) from the basal cell to the other part of the embryo proper and from the basal cell to the endosperm is also restricted; (c) the embryo is a single symplasmic domain with respect to molecules of a molecular weight below 0.5 kDa. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00425-016-2619-y) contains supplementary material, which is available to authorized users. Springer Berlin Heidelberg 2016-11-25 2017 /pmc/articles/PMC5310571/ /pubmed/27888360 http://dx.doi.org/10.1007/s00425-016-2619-y Text en © The Author(s) 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Original Article
Wróbel-Marek, Justyna
Kurczyńska, Ewa
Płachno, Bartosz J.
Kozieradzka-Kiszkurno, Małgorzata
Identification of symplasmic domains in the embryo and seed of Sedum acre L. (Crassulaceae)
title Identification of symplasmic domains in the embryo and seed of Sedum acre L. (Crassulaceae)
title_full Identification of symplasmic domains in the embryo and seed of Sedum acre L. (Crassulaceae)
title_fullStr Identification of symplasmic domains in the embryo and seed of Sedum acre L. (Crassulaceae)
title_full_unstemmed Identification of symplasmic domains in the embryo and seed of Sedum acre L. (Crassulaceae)
title_short Identification of symplasmic domains in the embryo and seed of Sedum acre L. (Crassulaceae)
title_sort identification of symplasmic domains in the embryo and seed of sedum acre l. (crassulaceae)
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5310571/
https://www.ncbi.nlm.nih.gov/pubmed/27888360
http://dx.doi.org/10.1007/s00425-016-2619-y
work_keys_str_mv AT wrobelmarekjustyna identificationofsymplasmicdomainsintheembryoandseedofsedumacrelcrassulaceae
AT kurczynskaewa identificationofsymplasmicdomainsintheembryoandseedofsedumacrelcrassulaceae
AT płachnobartoszj identificationofsymplasmicdomainsintheembryoandseedofsedumacrelcrassulaceae
AT kozieradzkakiszkurnomałgorzata identificationofsymplasmicdomainsintheembryoandseedofsedumacrelcrassulaceae