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The use of urinary proteomics in the assessment of suitability of mouse models for ageing

Ageing is a complex process characterised by a systemic and progressive deterioration of biological functions. As ageing is associated with an increased prevalence of age-related chronic disorders, understanding its underlying molecular mechanisms can pave the way for therapeutic interventions and m...

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Autores principales: Nkuipou-Kenfack, Esther, Schanstra, Joost P., Bajwa, Seerat, Pejchinovski, Martin, Vinel, Claire, Dray, Cédric, Valet, Philippe, Bascands, Jean-Loup, Vlahou, Antonia, Koeck, Thomas, Borries, Melanie, Busch, Hauke, Bechtel-Walz, Wibke, Huber, Tobias B., Rudolph, Karl L., Pich, Andreas, Mischak, Harald, Zürbig, Petra
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5310860/
https://www.ncbi.nlm.nih.gov/pubmed/28199320
http://dx.doi.org/10.1371/journal.pone.0166875
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author Nkuipou-Kenfack, Esther
Schanstra, Joost P.
Bajwa, Seerat
Pejchinovski, Martin
Vinel, Claire
Dray, Cédric
Valet, Philippe
Bascands, Jean-Loup
Vlahou, Antonia
Koeck, Thomas
Borries, Melanie
Busch, Hauke
Bechtel-Walz, Wibke
Huber, Tobias B.
Rudolph, Karl L.
Pich, Andreas
Mischak, Harald
Zürbig, Petra
author_facet Nkuipou-Kenfack, Esther
Schanstra, Joost P.
Bajwa, Seerat
Pejchinovski, Martin
Vinel, Claire
Dray, Cédric
Valet, Philippe
Bascands, Jean-Loup
Vlahou, Antonia
Koeck, Thomas
Borries, Melanie
Busch, Hauke
Bechtel-Walz, Wibke
Huber, Tobias B.
Rudolph, Karl L.
Pich, Andreas
Mischak, Harald
Zürbig, Petra
author_sort Nkuipou-Kenfack, Esther
collection PubMed
description Ageing is a complex process characterised by a systemic and progressive deterioration of biological functions. As ageing is associated with an increased prevalence of age-related chronic disorders, understanding its underlying molecular mechanisms can pave the way for therapeutic interventions and managing complications. Animal models such as mice are commonly used in ageing research as they have a shorter lifespan in comparison to humans and are also genetically close to humans. To assess the translatability of mouse ageing to human ageing, the urinary proteome in 89 wild-type (C57BL/6) mice aged between 8–96 weeks was investigated using capillary electrophoresis coupled to mass spectrometry (CE-MS). Using age as a continuous variable, 295 peptides significantly correlated with age in mice were identified. To investigate the relevance of using mouse models in human ageing studies, a comparison was performed with a previous correlation analysis using 1227 healthy subjects. In mice and humans, a decrease in urinary excretion of fibrillar collagens and an increase of uromodulin fragments was observed with advanced age. Of the 295 peptides correlating with age, 49 had a strong homology to the respective human age-related peptides. These ortholog peptides including several collagen (N = 44) and uromodulin (N = 5) fragments were used to generate an ageing classifier that was able to discriminate the age among both wild-type mice and healthy subjects. Additionally, the ageing classifier depicted that telomerase knock-out mice were older than their chronological age. Hence, with a focus on ortholog urinary peptides mouse ageing can be translated to human ageing.
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spelling pubmed-53108602017-03-03 The use of urinary proteomics in the assessment of suitability of mouse models for ageing Nkuipou-Kenfack, Esther Schanstra, Joost P. Bajwa, Seerat Pejchinovski, Martin Vinel, Claire Dray, Cédric Valet, Philippe Bascands, Jean-Loup Vlahou, Antonia Koeck, Thomas Borries, Melanie Busch, Hauke Bechtel-Walz, Wibke Huber, Tobias B. Rudolph, Karl L. Pich, Andreas Mischak, Harald Zürbig, Petra PLoS One Research Article Ageing is a complex process characterised by a systemic and progressive deterioration of biological functions. As ageing is associated with an increased prevalence of age-related chronic disorders, understanding its underlying molecular mechanisms can pave the way for therapeutic interventions and managing complications. Animal models such as mice are commonly used in ageing research as they have a shorter lifespan in comparison to humans and are also genetically close to humans. To assess the translatability of mouse ageing to human ageing, the urinary proteome in 89 wild-type (C57BL/6) mice aged between 8–96 weeks was investigated using capillary electrophoresis coupled to mass spectrometry (CE-MS). Using age as a continuous variable, 295 peptides significantly correlated with age in mice were identified. To investigate the relevance of using mouse models in human ageing studies, a comparison was performed with a previous correlation analysis using 1227 healthy subjects. In mice and humans, a decrease in urinary excretion of fibrillar collagens and an increase of uromodulin fragments was observed with advanced age. Of the 295 peptides correlating with age, 49 had a strong homology to the respective human age-related peptides. These ortholog peptides including several collagen (N = 44) and uromodulin (N = 5) fragments were used to generate an ageing classifier that was able to discriminate the age among both wild-type mice and healthy subjects. Additionally, the ageing classifier depicted that telomerase knock-out mice were older than their chronological age. Hence, with a focus on ortholog urinary peptides mouse ageing can be translated to human ageing. Public Library of Science 2017-02-15 /pmc/articles/PMC5310860/ /pubmed/28199320 http://dx.doi.org/10.1371/journal.pone.0166875 Text en © 2017 Nkuipou-Kenfack et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Nkuipou-Kenfack, Esther
Schanstra, Joost P.
Bajwa, Seerat
Pejchinovski, Martin
Vinel, Claire
Dray, Cédric
Valet, Philippe
Bascands, Jean-Loup
Vlahou, Antonia
Koeck, Thomas
Borries, Melanie
Busch, Hauke
Bechtel-Walz, Wibke
Huber, Tobias B.
Rudolph, Karl L.
Pich, Andreas
Mischak, Harald
Zürbig, Petra
The use of urinary proteomics in the assessment of suitability of mouse models for ageing
title The use of urinary proteomics in the assessment of suitability of mouse models for ageing
title_full The use of urinary proteomics in the assessment of suitability of mouse models for ageing
title_fullStr The use of urinary proteomics in the assessment of suitability of mouse models for ageing
title_full_unstemmed The use of urinary proteomics in the assessment of suitability of mouse models for ageing
title_short The use of urinary proteomics in the assessment of suitability of mouse models for ageing
title_sort use of urinary proteomics in the assessment of suitability of mouse models for ageing
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5310860/
https://www.ncbi.nlm.nih.gov/pubmed/28199320
http://dx.doi.org/10.1371/journal.pone.0166875
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