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The use of urinary proteomics in the assessment of suitability of mouse models for ageing
Ageing is a complex process characterised by a systemic and progressive deterioration of biological functions. As ageing is associated with an increased prevalence of age-related chronic disorders, understanding its underlying molecular mechanisms can pave the way for therapeutic interventions and m...
Autores principales: | , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5310860/ https://www.ncbi.nlm.nih.gov/pubmed/28199320 http://dx.doi.org/10.1371/journal.pone.0166875 |
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author | Nkuipou-Kenfack, Esther Schanstra, Joost P. Bajwa, Seerat Pejchinovski, Martin Vinel, Claire Dray, Cédric Valet, Philippe Bascands, Jean-Loup Vlahou, Antonia Koeck, Thomas Borries, Melanie Busch, Hauke Bechtel-Walz, Wibke Huber, Tobias B. Rudolph, Karl L. Pich, Andreas Mischak, Harald Zürbig, Petra |
author_facet | Nkuipou-Kenfack, Esther Schanstra, Joost P. Bajwa, Seerat Pejchinovski, Martin Vinel, Claire Dray, Cédric Valet, Philippe Bascands, Jean-Loup Vlahou, Antonia Koeck, Thomas Borries, Melanie Busch, Hauke Bechtel-Walz, Wibke Huber, Tobias B. Rudolph, Karl L. Pich, Andreas Mischak, Harald Zürbig, Petra |
author_sort | Nkuipou-Kenfack, Esther |
collection | PubMed |
description | Ageing is a complex process characterised by a systemic and progressive deterioration of biological functions. As ageing is associated with an increased prevalence of age-related chronic disorders, understanding its underlying molecular mechanisms can pave the way for therapeutic interventions and managing complications. Animal models such as mice are commonly used in ageing research as they have a shorter lifespan in comparison to humans and are also genetically close to humans. To assess the translatability of mouse ageing to human ageing, the urinary proteome in 89 wild-type (C57BL/6) mice aged between 8–96 weeks was investigated using capillary electrophoresis coupled to mass spectrometry (CE-MS). Using age as a continuous variable, 295 peptides significantly correlated with age in mice were identified. To investigate the relevance of using mouse models in human ageing studies, a comparison was performed with a previous correlation analysis using 1227 healthy subjects. In mice and humans, a decrease in urinary excretion of fibrillar collagens and an increase of uromodulin fragments was observed with advanced age. Of the 295 peptides correlating with age, 49 had a strong homology to the respective human age-related peptides. These ortholog peptides including several collagen (N = 44) and uromodulin (N = 5) fragments were used to generate an ageing classifier that was able to discriminate the age among both wild-type mice and healthy subjects. Additionally, the ageing classifier depicted that telomerase knock-out mice were older than their chronological age. Hence, with a focus on ortholog urinary peptides mouse ageing can be translated to human ageing. |
format | Online Article Text |
id | pubmed-5310860 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-53108602017-03-03 The use of urinary proteomics in the assessment of suitability of mouse models for ageing Nkuipou-Kenfack, Esther Schanstra, Joost P. Bajwa, Seerat Pejchinovski, Martin Vinel, Claire Dray, Cédric Valet, Philippe Bascands, Jean-Loup Vlahou, Antonia Koeck, Thomas Borries, Melanie Busch, Hauke Bechtel-Walz, Wibke Huber, Tobias B. Rudolph, Karl L. Pich, Andreas Mischak, Harald Zürbig, Petra PLoS One Research Article Ageing is a complex process characterised by a systemic and progressive deterioration of biological functions. As ageing is associated with an increased prevalence of age-related chronic disorders, understanding its underlying molecular mechanisms can pave the way for therapeutic interventions and managing complications. Animal models such as mice are commonly used in ageing research as they have a shorter lifespan in comparison to humans and are also genetically close to humans. To assess the translatability of mouse ageing to human ageing, the urinary proteome in 89 wild-type (C57BL/6) mice aged between 8–96 weeks was investigated using capillary electrophoresis coupled to mass spectrometry (CE-MS). Using age as a continuous variable, 295 peptides significantly correlated with age in mice were identified. To investigate the relevance of using mouse models in human ageing studies, a comparison was performed with a previous correlation analysis using 1227 healthy subjects. In mice and humans, a decrease in urinary excretion of fibrillar collagens and an increase of uromodulin fragments was observed with advanced age. Of the 295 peptides correlating with age, 49 had a strong homology to the respective human age-related peptides. These ortholog peptides including several collagen (N = 44) and uromodulin (N = 5) fragments were used to generate an ageing classifier that was able to discriminate the age among both wild-type mice and healthy subjects. Additionally, the ageing classifier depicted that telomerase knock-out mice were older than their chronological age. Hence, with a focus on ortholog urinary peptides mouse ageing can be translated to human ageing. Public Library of Science 2017-02-15 /pmc/articles/PMC5310860/ /pubmed/28199320 http://dx.doi.org/10.1371/journal.pone.0166875 Text en © 2017 Nkuipou-Kenfack et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Nkuipou-Kenfack, Esther Schanstra, Joost P. Bajwa, Seerat Pejchinovski, Martin Vinel, Claire Dray, Cédric Valet, Philippe Bascands, Jean-Loup Vlahou, Antonia Koeck, Thomas Borries, Melanie Busch, Hauke Bechtel-Walz, Wibke Huber, Tobias B. Rudolph, Karl L. Pich, Andreas Mischak, Harald Zürbig, Petra The use of urinary proteomics in the assessment of suitability of mouse models for ageing |
title | The use of urinary proteomics in the assessment of suitability of mouse models for ageing |
title_full | The use of urinary proteomics in the assessment of suitability of mouse models for ageing |
title_fullStr | The use of urinary proteomics in the assessment of suitability of mouse models for ageing |
title_full_unstemmed | The use of urinary proteomics in the assessment of suitability of mouse models for ageing |
title_short | The use of urinary proteomics in the assessment of suitability of mouse models for ageing |
title_sort | use of urinary proteomics in the assessment of suitability of mouse models for ageing |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5310860/ https://www.ncbi.nlm.nih.gov/pubmed/28199320 http://dx.doi.org/10.1371/journal.pone.0166875 |
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