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Physical activity prevents alterations in mitochondrial ultrastructure and glucometabolic parameters in a high-sugar diet model
Endurance exercise is a remarkable intervention for the treatment of many diseases. Mitochondrial changes on skeletal muscle are likely important for many of the benefits provided by exercise. In this study, we aimed to evaluate the effects that a regular physical activity (swimming without workload...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5310863/ https://www.ncbi.nlm.nih.gov/pubmed/28199417 http://dx.doi.org/10.1371/journal.pone.0172103 |
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author | Barbosa de Queiroz, Karina Honorato-Sampaio, Kinulpe Rossoni Júnior, Joamyr Victor Andrade Leal, Diego Pinto, Angélica Barbosa G. Kappes-Becker, Lenice Evangelista, Elisio Alberto Guerra-Sá, Renata |
author_facet | Barbosa de Queiroz, Karina Honorato-Sampaio, Kinulpe Rossoni Júnior, Joamyr Victor Andrade Leal, Diego Pinto, Angélica Barbosa G. Kappes-Becker, Lenice Evangelista, Elisio Alberto Guerra-Sá, Renata |
author_sort | Barbosa de Queiroz, Karina |
collection | PubMed |
description | Endurance exercise is a remarkable intervention for the treatment of many diseases. Mitochondrial changes on skeletal muscle are likely important for many of the benefits provided by exercise. In this study, we aimed to evaluate the effects that a regular physical activity (swimming without workload) has on mitochondrial morphological alterations and glucometabolic parameters induced by a high-sugar diet (HSD). Weaned male Wistar rats fed with a standard diet or a HSD (68% carbohydrate) were subjected to 60 minutes of regular physical activity by swimming (without workload) for four- (20 sessions) or eight-week (40 sessions) periods. After training, animals were euthanized and the sera, adipose tissues, and skeletal muscles were collected for further analysis. The HSD increased body weight after an 8-week period; it also increased the fat pads and the adipose index, resulting in glucose intolerance and insulin resistance (IR). Transmission electron microscopy showed an increase in alterations of mitochondrial ultrastructure in the gastrocnemius muscle, as well as a decrease in superoxide dismutase (SOD) activity, and an increase in protein carbonylation. Regular physical activity partially reverted these alterations in rats fed a HSD, preventing mitochondrial morphological alterations and IR. Moreover, we observed a decrease in Pgc1α expression (qPCR analysis) in STD-EXE group and a less pronounced reduction in HSD-EXE group after an 8-week period. Thus, regular physical activity (swimming without workload) in rats fed a HSD can prevent mitochondrial dysfunction and IR, highlighting the crucial role for physical activity on metabolic homeostasis. |
format | Online Article Text |
id | pubmed-5310863 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-53108632017-03-03 Physical activity prevents alterations in mitochondrial ultrastructure and glucometabolic parameters in a high-sugar diet model Barbosa de Queiroz, Karina Honorato-Sampaio, Kinulpe Rossoni Júnior, Joamyr Victor Andrade Leal, Diego Pinto, Angélica Barbosa G. Kappes-Becker, Lenice Evangelista, Elisio Alberto Guerra-Sá, Renata PLoS One Research Article Endurance exercise is a remarkable intervention for the treatment of many diseases. Mitochondrial changes on skeletal muscle are likely important for many of the benefits provided by exercise. In this study, we aimed to evaluate the effects that a regular physical activity (swimming without workload) has on mitochondrial morphological alterations and glucometabolic parameters induced by a high-sugar diet (HSD). Weaned male Wistar rats fed with a standard diet or a HSD (68% carbohydrate) were subjected to 60 minutes of regular physical activity by swimming (without workload) for four- (20 sessions) or eight-week (40 sessions) periods. After training, animals were euthanized and the sera, adipose tissues, and skeletal muscles were collected for further analysis. The HSD increased body weight after an 8-week period; it also increased the fat pads and the adipose index, resulting in glucose intolerance and insulin resistance (IR). Transmission electron microscopy showed an increase in alterations of mitochondrial ultrastructure in the gastrocnemius muscle, as well as a decrease in superoxide dismutase (SOD) activity, and an increase in protein carbonylation. Regular physical activity partially reverted these alterations in rats fed a HSD, preventing mitochondrial morphological alterations and IR. Moreover, we observed a decrease in Pgc1α expression (qPCR analysis) in STD-EXE group and a less pronounced reduction in HSD-EXE group after an 8-week period. Thus, regular physical activity (swimming without workload) in rats fed a HSD can prevent mitochondrial dysfunction and IR, highlighting the crucial role for physical activity on metabolic homeostasis. Public Library of Science 2017-02-15 /pmc/articles/PMC5310863/ /pubmed/28199417 http://dx.doi.org/10.1371/journal.pone.0172103 Text en © 2017 Barbosa de Queiroz et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Barbosa de Queiroz, Karina Honorato-Sampaio, Kinulpe Rossoni Júnior, Joamyr Victor Andrade Leal, Diego Pinto, Angélica Barbosa G. Kappes-Becker, Lenice Evangelista, Elisio Alberto Guerra-Sá, Renata Physical activity prevents alterations in mitochondrial ultrastructure and glucometabolic parameters in a high-sugar diet model |
title | Physical activity prevents alterations in mitochondrial ultrastructure and glucometabolic parameters in a high-sugar diet model |
title_full | Physical activity prevents alterations in mitochondrial ultrastructure and glucometabolic parameters in a high-sugar diet model |
title_fullStr | Physical activity prevents alterations in mitochondrial ultrastructure and glucometabolic parameters in a high-sugar diet model |
title_full_unstemmed | Physical activity prevents alterations in mitochondrial ultrastructure and glucometabolic parameters in a high-sugar diet model |
title_short | Physical activity prevents alterations in mitochondrial ultrastructure and glucometabolic parameters in a high-sugar diet model |
title_sort | physical activity prevents alterations in mitochondrial ultrastructure and glucometabolic parameters in a high-sugar diet model |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5310863/ https://www.ncbi.nlm.nih.gov/pubmed/28199417 http://dx.doi.org/10.1371/journal.pone.0172103 |
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