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Long-term culture-induced phenotypic difference and efficient cryopreservation of small intestinal organoids by treatment timing of Rho kinase inhibitor
AIM: To investigate a suitable long-term culture system and optimal cryopreservation of intestinal organoid to improve organoid-based therapy by acquiring large numbers of cells. METHODS: Crypts were isolated from jejunum of C57BL/6 mouse. Two hundred crypts were cultured in organoid medium with eit...
Autores principales: | Han, Sung-Hoon, Shim, Sehwan, Kim, Min-Jung, Shin, Hye-Yun, Jang, Won-Suk, Lee, Sun-Joo, Jin, Young-Woo, Lee, Seung-Sook, Lee, Seung Bum, Park, Sunhoo |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Baishideng Publishing Group Inc
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5311106/ https://www.ncbi.nlm.nih.gov/pubmed/28246470 http://dx.doi.org/10.3748/wjg.v23.i6.964 |
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