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Nup100 regulates Saccharomyces cerevisiae replicative life span by mediating the nuclear export of specific tRNAs
Nuclear pore complexes (NPCs), which are composed of nucleoporins (Nups) and regulate transport between the nucleus and cytoplasm, significantly impact the replicative life span (RLS) of Saccharomyces cerevisiae. We previously reported that deletion of the nonessential gene NUP100 increases RLS, alt...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cold Spring Harbor Laboratory Press
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5311497/ https://www.ncbi.nlm.nih.gov/pubmed/27932586 http://dx.doi.org/10.1261/rna.057612.116 |
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author | Lord, Christopher L. Ospovat, Ophir Wente, Susan R. |
author_facet | Lord, Christopher L. Ospovat, Ophir Wente, Susan R. |
author_sort | Lord, Christopher L. |
collection | PubMed |
description | Nuclear pore complexes (NPCs), which are composed of nucleoporins (Nups) and regulate transport between the nucleus and cytoplasm, significantly impact the replicative life span (RLS) of Saccharomyces cerevisiae. We previously reported that deletion of the nonessential gene NUP100 increases RLS, although the molecular basis for this effect was unknown. In this study, we find that nuclear tRNA accumulation contributes to increased longevity in nup100Δ cells. Fluorescence in situ hybridization (FISH) experiments demonstrate that several specific tRNAs accumulate in the nuclei of nup100Δ mutants. Protein levels of the transcription factor Gcn4 are increased when NUP100 is deleted, and GCN4 is required for the elevated life spans of nup100Δ mutants, similar to other previously described tRNA export and ribosomal mutants. Northern blots indicate that tRNA splicing and aminoacylation are not significantly affected in nup100Δ cells, suggesting that Nup100 is largely required for nuclear export of mature, processed tRNAs. Distinct tRNAs accumulate in the nuclei of nup100Δ and msn5Δ mutants, while Los1-GFP nucleocytoplasmic shuttling is unaffected by Nup100. Thus, we conclude that Nup100 regulates tRNA export in a manner distinct from Los1 or Msn5. Together, these experiments reveal a novel Nup100 role in the tRNA life cycle that impacts the S. cerevisiae life span. |
format | Online Article Text |
id | pubmed-5311497 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Cold Spring Harbor Laboratory Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-53114972018-03-01 Nup100 regulates Saccharomyces cerevisiae replicative life span by mediating the nuclear export of specific tRNAs Lord, Christopher L. Ospovat, Ophir Wente, Susan R. RNA Article Nuclear pore complexes (NPCs), which are composed of nucleoporins (Nups) and regulate transport between the nucleus and cytoplasm, significantly impact the replicative life span (RLS) of Saccharomyces cerevisiae. We previously reported that deletion of the nonessential gene NUP100 increases RLS, although the molecular basis for this effect was unknown. In this study, we find that nuclear tRNA accumulation contributes to increased longevity in nup100Δ cells. Fluorescence in situ hybridization (FISH) experiments demonstrate that several specific tRNAs accumulate in the nuclei of nup100Δ mutants. Protein levels of the transcription factor Gcn4 are increased when NUP100 is deleted, and GCN4 is required for the elevated life spans of nup100Δ mutants, similar to other previously described tRNA export and ribosomal mutants. Northern blots indicate that tRNA splicing and aminoacylation are not significantly affected in nup100Δ cells, suggesting that Nup100 is largely required for nuclear export of mature, processed tRNAs. Distinct tRNAs accumulate in the nuclei of nup100Δ and msn5Δ mutants, while Los1-GFP nucleocytoplasmic shuttling is unaffected by Nup100. Thus, we conclude that Nup100 regulates tRNA export in a manner distinct from Los1 or Msn5. Together, these experiments reveal a novel Nup100 role in the tRNA life cycle that impacts the S. cerevisiae life span. Cold Spring Harbor Laboratory Press 2017-03 /pmc/articles/PMC5311497/ /pubmed/27932586 http://dx.doi.org/10.1261/rna.057612.116 Text en © 2017 Lord et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society http://creativecommons.org/licenses/by-nc/4.0/ This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/. |
spellingShingle | Article Lord, Christopher L. Ospovat, Ophir Wente, Susan R. Nup100 regulates Saccharomyces cerevisiae replicative life span by mediating the nuclear export of specific tRNAs |
title | Nup100 regulates Saccharomyces cerevisiae replicative life span by mediating the nuclear export of specific tRNAs |
title_full | Nup100 regulates Saccharomyces cerevisiae replicative life span by mediating the nuclear export of specific tRNAs |
title_fullStr | Nup100 regulates Saccharomyces cerevisiae replicative life span by mediating the nuclear export of specific tRNAs |
title_full_unstemmed | Nup100 regulates Saccharomyces cerevisiae replicative life span by mediating the nuclear export of specific tRNAs |
title_short | Nup100 regulates Saccharomyces cerevisiae replicative life span by mediating the nuclear export of specific tRNAs |
title_sort | nup100 regulates saccharomyces cerevisiae replicative life span by mediating the nuclear export of specific trnas |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5311497/ https://www.ncbi.nlm.nih.gov/pubmed/27932586 http://dx.doi.org/10.1261/rna.057612.116 |
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