Cargando…

Nup100 regulates Saccharomyces cerevisiae replicative life span by mediating the nuclear export of specific tRNAs

Nuclear pore complexes (NPCs), which are composed of nucleoporins (Nups) and regulate transport between the nucleus and cytoplasm, significantly impact the replicative life span (RLS) of Saccharomyces cerevisiae. We previously reported that deletion of the nonessential gene NUP100 increases RLS, alt...

Descripción completa

Detalles Bibliográficos
Autores principales: Lord, Christopher L., Ospovat, Ophir, Wente, Susan R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5311497/
https://www.ncbi.nlm.nih.gov/pubmed/27932586
http://dx.doi.org/10.1261/rna.057612.116
_version_ 1782508032834404352
author Lord, Christopher L.
Ospovat, Ophir
Wente, Susan R.
author_facet Lord, Christopher L.
Ospovat, Ophir
Wente, Susan R.
author_sort Lord, Christopher L.
collection PubMed
description Nuclear pore complexes (NPCs), which are composed of nucleoporins (Nups) and regulate transport between the nucleus and cytoplasm, significantly impact the replicative life span (RLS) of Saccharomyces cerevisiae. We previously reported that deletion of the nonessential gene NUP100 increases RLS, although the molecular basis for this effect was unknown. In this study, we find that nuclear tRNA accumulation contributes to increased longevity in nup100Δ cells. Fluorescence in situ hybridization (FISH) experiments demonstrate that several specific tRNAs accumulate in the nuclei of nup100Δ mutants. Protein levels of the transcription factor Gcn4 are increased when NUP100 is deleted, and GCN4 is required for the elevated life spans of nup100Δ mutants, similar to other previously described tRNA export and ribosomal mutants. Northern blots indicate that tRNA splicing and aminoacylation are not significantly affected in nup100Δ cells, suggesting that Nup100 is largely required for nuclear export of mature, processed tRNAs. Distinct tRNAs accumulate in the nuclei of nup100Δ and msn5Δ mutants, while Los1-GFP nucleocytoplasmic shuttling is unaffected by Nup100. Thus, we conclude that Nup100 regulates tRNA export in a manner distinct from Los1 or Msn5. Together, these experiments reveal a novel Nup100 role in the tRNA life cycle that impacts the S. cerevisiae life span.
format Online
Article
Text
id pubmed-5311497
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Cold Spring Harbor Laboratory Press
record_format MEDLINE/PubMed
spelling pubmed-53114972018-03-01 Nup100 regulates Saccharomyces cerevisiae replicative life span by mediating the nuclear export of specific tRNAs Lord, Christopher L. Ospovat, Ophir Wente, Susan R. RNA Article Nuclear pore complexes (NPCs), which are composed of nucleoporins (Nups) and regulate transport between the nucleus and cytoplasm, significantly impact the replicative life span (RLS) of Saccharomyces cerevisiae. We previously reported that deletion of the nonessential gene NUP100 increases RLS, although the molecular basis for this effect was unknown. In this study, we find that nuclear tRNA accumulation contributes to increased longevity in nup100Δ cells. Fluorescence in situ hybridization (FISH) experiments demonstrate that several specific tRNAs accumulate in the nuclei of nup100Δ mutants. Protein levels of the transcription factor Gcn4 are increased when NUP100 is deleted, and GCN4 is required for the elevated life spans of nup100Δ mutants, similar to other previously described tRNA export and ribosomal mutants. Northern blots indicate that tRNA splicing and aminoacylation are not significantly affected in nup100Δ cells, suggesting that Nup100 is largely required for nuclear export of mature, processed tRNAs. Distinct tRNAs accumulate in the nuclei of nup100Δ and msn5Δ mutants, while Los1-GFP nucleocytoplasmic shuttling is unaffected by Nup100. Thus, we conclude that Nup100 regulates tRNA export in a manner distinct from Los1 or Msn5. Together, these experiments reveal a novel Nup100 role in the tRNA life cycle that impacts the S. cerevisiae life span. Cold Spring Harbor Laboratory Press 2017-03 /pmc/articles/PMC5311497/ /pubmed/27932586 http://dx.doi.org/10.1261/rna.057612.116 Text en © 2017 Lord et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society http://creativecommons.org/licenses/by-nc/4.0/ This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.
spellingShingle Article
Lord, Christopher L.
Ospovat, Ophir
Wente, Susan R.
Nup100 regulates Saccharomyces cerevisiae replicative life span by mediating the nuclear export of specific tRNAs
title Nup100 regulates Saccharomyces cerevisiae replicative life span by mediating the nuclear export of specific tRNAs
title_full Nup100 regulates Saccharomyces cerevisiae replicative life span by mediating the nuclear export of specific tRNAs
title_fullStr Nup100 regulates Saccharomyces cerevisiae replicative life span by mediating the nuclear export of specific tRNAs
title_full_unstemmed Nup100 regulates Saccharomyces cerevisiae replicative life span by mediating the nuclear export of specific tRNAs
title_short Nup100 regulates Saccharomyces cerevisiae replicative life span by mediating the nuclear export of specific tRNAs
title_sort nup100 regulates saccharomyces cerevisiae replicative life span by mediating the nuclear export of specific trnas
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5311497/
https://www.ncbi.nlm.nih.gov/pubmed/27932586
http://dx.doi.org/10.1261/rna.057612.116
work_keys_str_mv AT lordchristopherl nup100regulatessaccharomycescerevisiaereplicativelifespanbymediatingthenuclearexportofspecifictrnas
AT ospovatophir nup100regulatessaccharomycescerevisiaereplicativelifespanbymediatingthenuclearexportofspecifictrnas
AT wentesusanr nup100regulatessaccharomycescerevisiaereplicativelifespanbymediatingthenuclearexportofspecifictrnas