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Efficient CRISPR/Cas9-assisted gene targeting enables rapid and precise genetic manipulation of mammalian neural stem cells

Mammalian neural stem cell (NSC) lines provide a tractable model for discovery across stem cell and developmental biology, regenerative medicine and neuroscience. They can be derived from foetal or adult germinal tissues and continuously propagated in vitro as adherent monolayers. NSCs are clonally...

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Detalles Bibliográficos
Autores principales:	Bressan, Raul Bardini, Dewari, Pooran Singh, Kalantzaki, Maria, Gangoso, Ester, Matjusaitis, Mantas, Garcia-Diaz, Claudia, Blin, Carla, Grant, Vivien, Bulstrode, Harry, Gogolok, Sabine, Skarnes, William C., Pollard, Steven M.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	The Company of Biologists Ltd 2017
Materias:	Stem Cells and Regeneration
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5312033/ https://www.ncbi.nlm.nih.gov/pubmed/28096221 http://dx.doi.org/10.1242/dev.140855

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