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Differential Site-Based Expression of Pentose Phosphate Pathway-Related Proteins among Breast Cancer Metastases
Purpose. We aimed to investigate the expression of pentose phosphate pathway- (PPP-) related proteins in metastatic breast cancer and its relationship with clinicopathologic factors. Methods. Tissue samples from 126 metastatic breast cancers were included in a tissue microarray. Expression of PPP-re...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5312075/ https://www.ncbi.nlm.nih.gov/pubmed/28260828 http://dx.doi.org/10.1155/2017/7062517 |
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author | Cha, Yoon Jin Jung, Woo Hee Koo, Ja Seung |
author_facet | Cha, Yoon Jin Jung, Woo Hee Koo, Ja Seung |
author_sort | Cha, Yoon Jin |
collection | PubMed |
description | Purpose. We aimed to investigate the expression of pentose phosphate pathway- (PPP-) related proteins in metastatic breast cancer and its relationship with clinicopathologic factors. Methods. Tissue samples from 126 metastatic breast cancers were included in a tissue microarray. Expression of PPP-related proteins [glucose-6-phosphate dehydrogenase (G6PDH), 6-phosphogluconolactonase (6PGL), 6-phosphogluconate dehydrogenase (6PGDH), and nuclear factor erythroid 2-related factor (NRF2)] was determined by immunohistochemistry. Results. G6PDH (p = 0.011) and cytoplasmic NRF2 (p = 0.001) showed the highest expression in brain metastases. Human epidermal growth factor receptor (HER-2) positivity was associated with G6PDH (p < 0.001) and cytoplasmic NRF2 (p = 0.015) positivity. A high Ki-67 labeling index (LI) was correlated with nuclear NRF2 positivity (p = 0.037), and HER-2-positive luminal B type was associated with G6PDH positivity (p = 0.001). On multivariate Cox analysis, independent risk factors of short overall survival were 6PGL positivity in bone metastasis (HR 4.180, 95% CI 1.160–15.06, p = 0.029) and low Ki-67 LI in lung metastasis (HR 11.853, 95% CI 1.841–76.30, p = 0.009). Conclusion. Differential expression of PPP-related proteins correlated with different prognoses and metastatic sites, with the highest expression in brain metastases, and could be a potential therapeutic target. |
format | Online Article Text |
id | pubmed-5312075 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-53120752017-03-05 Differential Site-Based Expression of Pentose Phosphate Pathway-Related Proteins among Breast Cancer Metastases Cha, Yoon Jin Jung, Woo Hee Koo, Ja Seung Dis Markers Research Article Purpose. We aimed to investigate the expression of pentose phosphate pathway- (PPP-) related proteins in metastatic breast cancer and its relationship with clinicopathologic factors. Methods. Tissue samples from 126 metastatic breast cancers were included in a tissue microarray. Expression of PPP-related proteins [glucose-6-phosphate dehydrogenase (G6PDH), 6-phosphogluconolactonase (6PGL), 6-phosphogluconate dehydrogenase (6PGDH), and nuclear factor erythroid 2-related factor (NRF2)] was determined by immunohistochemistry. Results. G6PDH (p = 0.011) and cytoplasmic NRF2 (p = 0.001) showed the highest expression in brain metastases. Human epidermal growth factor receptor (HER-2) positivity was associated with G6PDH (p < 0.001) and cytoplasmic NRF2 (p = 0.015) positivity. A high Ki-67 labeling index (LI) was correlated with nuclear NRF2 positivity (p = 0.037), and HER-2-positive luminal B type was associated with G6PDH positivity (p = 0.001). On multivariate Cox analysis, independent risk factors of short overall survival were 6PGL positivity in bone metastasis (HR 4.180, 95% CI 1.160–15.06, p = 0.029) and low Ki-67 LI in lung metastasis (HR 11.853, 95% CI 1.841–76.30, p = 0.009). Conclusion. Differential expression of PPP-related proteins correlated with different prognoses and metastatic sites, with the highest expression in brain metastases, and could be a potential therapeutic target. Hindawi Publishing Corporation 2017 2017-02-02 /pmc/articles/PMC5312075/ /pubmed/28260828 http://dx.doi.org/10.1155/2017/7062517 Text en Copyright © 2017 Yoon Jin Cha et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Cha, Yoon Jin Jung, Woo Hee Koo, Ja Seung Differential Site-Based Expression of Pentose Phosphate Pathway-Related Proteins among Breast Cancer Metastases |
title | Differential Site-Based Expression of Pentose Phosphate Pathway-Related Proteins among Breast Cancer Metastases |
title_full | Differential Site-Based Expression of Pentose Phosphate Pathway-Related Proteins among Breast Cancer Metastases |
title_fullStr | Differential Site-Based Expression of Pentose Phosphate Pathway-Related Proteins among Breast Cancer Metastases |
title_full_unstemmed | Differential Site-Based Expression of Pentose Phosphate Pathway-Related Proteins among Breast Cancer Metastases |
title_short | Differential Site-Based Expression of Pentose Phosphate Pathway-Related Proteins among Breast Cancer Metastases |
title_sort | differential site-based expression of pentose phosphate pathway-related proteins among breast cancer metastases |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5312075/ https://www.ncbi.nlm.nih.gov/pubmed/28260828 http://dx.doi.org/10.1155/2017/7062517 |
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