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Dynamic analysis of the mesenchymal-epithelial transition of blood-brain barrier forming glia in Drosophila

During development, many epithelia are formed by a mesenchymal-epithelial transition (MET). Here, we examine the major stages and underlying mechanisms of MET during blood-brain barrier formation in Drosophila. We show that contact with the basal lamina is essential for the growth of the barrier-for...

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Autores principales: Schwabe, Tina, Li, Xiaoling, Gaul, Ulrike
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Company of Biologists Ltd 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5312092/
https://www.ncbi.nlm.nih.gov/pubmed/28108476
http://dx.doi.org/10.1242/bio.020669
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author Schwabe, Tina
Li, Xiaoling
Gaul, Ulrike
author_facet Schwabe, Tina
Li, Xiaoling
Gaul, Ulrike
author_sort Schwabe, Tina
collection PubMed
description During development, many epithelia are formed by a mesenchymal-epithelial transition (MET). Here, we examine the major stages and underlying mechanisms of MET during blood-brain barrier formation in Drosophila. We show that contact with the basal lamina is essential for the growth of the barrier-forming subperineurial glia (SPG). Septate junctions (SJs), which provide insulation of the paracellular space, are not required for MET, but are necessary for the establishment of polarized SPG membrane compartments. In vivo time-lapse imaging reveals that the Moody GPCR signaling pathway regulates SPG cell growth and shape, with different levels of signaling causing distinct phenotypes. Timely, well-coordinated SPG growth is essential for the uniform insertion of SJs and thus the insulating function of the barrier. To our knowledge, this is the first dynamic in vivo analysis of all stages in the formation of a secondary epithelium, and of the key role trimeric G protein signaling plays in this important morphogenetic process.
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spelling pubmed-53120922017-03-06 Dynamic analysis of the mesenchymal-epithelial transition of blood-brain barrier forming glia in Drosophila Schwabe, Tina Li, Xiaoling Gaul, Ulrike Biol Open Research Article During development, many epithelia are formed by a mesenchymal-epithelial transition (MET). Here, we examine the major stages and underlying mechanisms of MET during blood-brain barrier formation in Drosophila. We show that contact with the basal lamina is essential for the growth of the barrier-forming subperineurial glia (SPG). Septate junctions (SJs), which provide insulation of the paracellular space, are not required for MET, but are necessary for the establishment of polarized SPG membrane compartments. In vivo time-lapse imaging reveals that the Moody GPCR signaling pathway regulates SPG cell growth and shape, with different levels of signaling causing distinct phenotypes. Timely, well-coordinated SPG growth is essential for the uniform insertion of SJs and thus the insulating function of the barrier. To our knowledge, this is the first dynamic in vivo analysis of all stages in the formation of a secondary epithelium, and of the key role trimeric G protein signaling plays in this important morphogenetic process. The Company of Biologists Ltd 2017-01-20 /pmc/articles/PMC5312092/ /pubmed/28108476 http://dx.doi.org/10.1242/bio.020669 Text en © 2017. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by/3.0This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
spellingShingle Research Article
Schwabe, Tina
Li, Xiaoling
Gaul, Ulrike
Dynamic analysis of the mesenchymal-epithelial transition of blood-brain barrier forming glia in Drosophila
title Dynamic analysis of the mesenchymal-epithelial transition of blood-brain barrier forming glia in Drosophila
title_full Dynamic analysis of the mesenchymal-epithelial transition of blood-brain barrier forming glia in Drosophila
title_fullStr Dynamic analysis of the mesenchymal-epithelial transition of blood-brain barrier forming glia in Drosophila
title_full_unstemmed Dynamic analysis of the mesenchymal-epithelial transition of blood-brain barrier forming glia in Drosophila
title_short Dynamic analysis of the mesenchymal-epithelial transition of blood-brain barrier forming glia in Drosophila
title_sort dynamic analysis of the mesenchymal-epithelial transition of blood-brain barrier forming glia in drosophila
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5312092/
https://www.ncbi.nlm.nih.gov/pubmed/28108476
http://dx.doi.org/10.1242/bio.020669
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