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Innovative approaches to establish and characterize primary cultures: an ex vivo 3D system and the zebrafish model

Patient-derived specimens are an invaluable resource to investigate tumor biology. However, in vivo studies on primary cultures are often limited by the small amount of material available, while conventional in vitro systems might alter the features and behavior that characterize cancer cells. We pr...

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Autores principales: Liverani, Chiara, La Manna, Federico, Groenewoud, Arwin, Mercatali, Laura, Van Der Pluijm, Gabri, Pieri, Federica, Cavaliere, Davide, De Vita, Alessandro, Spadazzi, Chiara, Miserocchi, Giacomo, Bongiovanni, Alberto, Recine, Federica, Riva, Nada, Amadori, Dino, Tasciotti, Ennio, Snaar-Jagalska, Ewa, Ibrahim, Toni
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Company of Biologists Ltd 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5312106/
https://www.ncbi.nlm.nih.gov/pubmed/27895047
http://dx.doi.org/10.1242/bio.022483
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author Liverani, Chiara
La Manna, Federico
Groenewoud, Arwin
Mercatali, Laura
Van Der Pluijm, Gabri
Pieri, Federica
Cavaliere, Davide
De Vita, Alessandro
Spadazzi, Chiara
Miserocchi, Giacomo
Bongiovanni, Alberto
Recine, Federica
Riva, Nada
Amadori, Dino
Tasciotti, Ennio
Snaar-Jagalska, Ewa
Ibrahim, Toni
author_facet Liverani, Chiara
La Manna, Federico
Groenewoud, Arwin
Mercatali, Laura
Van Der Pluijm, Gabri
Pieri, Federica
Cavaliere, Davide
De Vita, Alessandro
Spadazzi, Chiara
Miserocchi, Giacomo
Bongiovanni, Alberto
Recine, Federica
Riva, Nada
Amadori, Dino
Tasciotti, Ennio
Snaar-Jagalska, Ewa
Ibrahim, Toni
author_sort Liverani, Chiara
collection PubMed
description Patient-derived specimens are an invaluable resource to investigate tumor biology. However, in vivo studies on primary cultures are often limited by the small amount of material available, while conventional in vitro systems might alter the features and behavior that characterize cancer cells. We present our data obtained on primary dedifferentiated liposarcoma cells cultured in a 3D scaffold-based system and injected into a zebrafish model. Primary cells were characterized in vitro for their morphological features, sensitivity to drugs and biomarker expression, and in vivo for their engraftment and invasiveness abilities. The 3D culture showed a higher enrichment in cancer cells than the standard monolayer culture and a better preservation of liposarcoma-associated markers. We also successfully grafted primary cells into zebrafish, showing their local migratory and invasive abilities. Our work provides proof of concept of the ability of 3D cultures to maintain the original phenotype of ex vivo cells, and highlights the potential of the zebrafish model to provide a versatile in vivo system for studies with limited biological material. Such models could be used in translational research studies for biomolecular analyses, drug screenings and tumor aggressiveness assays.
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spelling pubmed-53121062017-03-06 Innovative approaches to establish and characterize primary cultures: an ex vivo 3D system and the zebrafish model Liverani, Chiara La Manna, Federico Groenewoud, Arwin Mercatali, Laura Van Der Pluijm, Gabri Pieri, Federica Cavaliere, Davide De Vita, Alessandro Spadazzi, Chiara Miserocchi, Giacomo Bongiovanni, Alberto Recine, Federica Riva, Nada Amadori, Dino Tasciotti, Ennio Snaar-Jagalska, Ewa Ibrahim, Toni Biol Open Research Article Patient-derived specimens are an invaluable resource to investigate tumor biology. However, in vivo studies on primary cultures are often limited by the small amount of material available, while conventional in vitro systems might alter the features and behavior that characterize cancer cells. We present our data obtained on primary dedifferentiated liposarcoma cells cultured in a 3D scaffold-based system and injected into a zebrafish model. Primary cells were characterized in vitro for their morphological features, sensitivity to drugs and biomarker expression, and in vivo for their engraftment and invasiveness abilities. The 3D culture showed a higher enrichment in cancer cells than the standard monolayer culture and a better preservation of liposarcoma-associated markers. We also successfully grafted primary cells into zebrafish, showing their local migratory and invasive abilities. Our work provides proof of concept of the ability of 3D cultures to maintain the original phenotype of ex vivo cells, and highlights the potential of the zebrafish model to provide a versatile in vivo system for studies with limited biological material. Such models could be used in translational research studies for biomolecular analyses, drug screenings and tumor aggressiveness assays. The Company of Biologists Ltd 2016-11-28 /pmc/articles/PMC5312106/ /pubmed/27895047 http://dx.doi.org/10.1242/bio.022483 Text en © 2016. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by/3.0This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
spellingShingle Research Article
Liverani, Chiara
La Manna, Federico
Groenewoud, Arwin
Mercatali, Laura
Van Der Pluijm, Gabri
Pieri, Federica
Cavaliere, Davide
De Vita, Alessandro
Spadazzi, Chiara
Miserocchi, Giacomo
Bongiovanni, Alberto
Recine, Federica
Riva, Nada
Amadori, Dino
Tasciotti, Ennio
Snaar-Jagalska, Ewa
Ibrahim, Toni
Innovative approaches to establish and characterize primary cultures: an ex vivo 3D system and the zebrafish model
title Innovative approaches to establish and characterize primary cultures: an ex vivo 3D system and the zebrafish model
title_full Innovative approaches to establish and characterize primary cultures: an ex vivo 3D system and the zebrafish model
title_fullStr Innovative approaches to establish and characterize primary cultures: an ex vivo 3D system and the zebrafish model
title_full_unstemmed Innovative approaches to establish and characterize primary cultures: an ex vivo 3D system and the zebrafish model
title_short Innovative approaches to establish and characterize primary cultures: an ex vivo 3D system and the zebrafish model
title_sort innovative approaches to establish and characterize primary cultures: an ex vivo 3d system and the zebrafish model
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5312106/
https://www.ncbi.nlm.nih.gov/pubmed/27895047
http://dx.doi.org/10.1242/bio.022483
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