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Innovative approaches to establish and characterize primary cultures: an ex vivo 3D system and the zebrafish model
Patient-derived specimens are an invaluable resource to investigate tumor biology. However, in vivo studies on primary cultures are often limited by the small amount of material available, while conventional in vitro systems might alter the features and behavior that characterize cancer cells. We pr...
Autores principales: | , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Company of Biologists Ltd
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5312106/ https://www.ncbi.nlm.nih.gov/pubmed/27895047 http://dx.doi.org/10.1242/bio.022483 |
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author | Liverani, Chiara La Manna, Federico Groenewoud, Arwin Mercatali, Laura Van Der Pluijm, Gabri Pieri, Federica Cavaliere, Davide De Vita, Alessandro Spadazzi, Chiara Miserocchi, Giacomo Bongiovanni, Alberto Recine, Federica Riva, Nada Amadori, Dino Tasciotti, Ennio Snaar-Jagalska, Ewa Ibrahim, Toni |
author_facet | Liverani, Chiara La Manna, Federico Groenewoud, Arwin Mercatali, Laura Van Der Pluijm, Gabri Pieri, Federica Cavaliere, Davide De Vita, Alessandro Spadazzi, Chiara Miserocchi, Giacomo Bongiovanni, Alberto Recine, Federica Riva, Nada Amadori, Dino Tasciotti, Ennio Snaar-Jagalska, Ewa Ibrahim, Toni |
author_sort | Liverani, Chiara |
collection | PubMed |
description | Patient-derived specimens are an invaluable resource to investigate tumor biology. However, in vivo studies on primary cultures are often limited by the small amount of material available, while conventional in vitro systems might alter the features and behavior that characterize cancer cells. We present our data obtained on primary dedifferentiated liposarcoma cells cultured in a 3D scaffold-based system and injected into a zebrafish model. Primary cells were characterized in vitro for their morphological features, sensitivity to drugs and biomarker expression, and in vivo for their engraftment and invasiveness abilities. The 3D culture showed a higher enrichment in cancer cells than the standard monolayer culture and a better preservation of liposarcoma-associated markers. We also successfully grafted primary cells into zebrafish, showing their local migratory and invasive abilities. Our work provides proof of concept of the ability of 3D cultures to maintain the original phenotype of ex vivo cells, and highlights the potential of the zebrafish model to provide a versatile in vivo system for studies with limited biological material. Such models could be used in translational research studies for biomolecular analyses, drug screenings and tumor aggressiveness assays. |
format | Online Article Text |
id | pubmed-5312106 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | The Company of Biologists Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-53121062017-03-06 Innovative approaches to establish and characterize primary cultures: an ex vivo 3D system and the zebrafish model Liverani, Chiara La Manna, Federico Groenewoud, Arwin Mercatali, Laura Van Der Pluijm, Gabri Pieri, Federica Cavaliere, Davide De Vita, Alessandro Spadazzi, Chiara Miserocchi, Giacomo Bongiovanni, Alberto Recine, Federica Riva, Nada Amadori, Dino Tasciotti, Ennio Snaar-Jagalska, Ewa Ibrahim, Toni Biol Open Research Article Patient-derived specimens are an invaluable resource to investigate tumor biology. However, in vivo studies on primary cultures are often limited by the small amount of material available, while conventional in vitro systems might alter the features and behavior that characterize cancer cells. We present our data obtained on primary dedifferentiated liposarcoma cells cultured in a 3D scaffold-based system and injected into a zebrafish model. Primary cells were characterized in vitro for their morphological features, sensitivity to drugs and biomarker expression, and in vivo for their engraftment and invasiveness abilities. The 3D culture showed a higher enrichment in cancer cells than the standard monolayer culture and a better preservation of liposarcoma-associated markers. We also successfully grafted primary cells into zebrafish, showing their local migratory and invasive abilities. Our work provides proof of concept of the ability of 3D cultures to maintain the original phenotype of ex vivo cells, and highlights the potential of the zebrafish model to provide a versatile in vivo system for studies with limited biological material. Such models could be used in translational research studies for biomolecular analyses, drug screenings and tumor aggressiveness assays. The Company of Biologists Ltd 2016-11-28 /pmc/articles/PMC5312106/ /pubmed/27895047 http://dx.doi.org/10.1242/bio.022483 Text en © 2016. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by/3.0This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. |
spellingShingle | Research Article Liverani, Chiara La Manna, Federico Groenewoud, Arwin Mercatali, Laura Van Der Pluijm, Gabri Pieri, Federica Cavaliere, Davide De Vita, Alessandro Spadazzi, Chiara Miserocchi, Giacomo Bongiovanni, Alberto Recine, Federica Riva, Nada Amadori, Dino Tasciotti, Ennio Snaar-Jagalska, Ewa Ibrahim, Toni Innovative approaches to establish and characterize primary cultures: an ex vivo 3D system and the zebrafish model |
title | Innovative approaches to establish and characterize primary cultures: an ex vivo 3D system and the zebrafish model |
title_full | Innovative approaches to establish and characterize primary cultures: an ex vivo 3D system and the zebrafish model |
title_fullStr | Innovative approaches to establish and characterize primary cultures: an ex vivo 3D system and the zebrafish model |
title_full_unstemmed | Innovative approaches to establish and characterize primary cultures: an ex vivo 3D system and the zebrafish model |
title_short | Innovative approaches to establish and characterize primary cultures: an ex vivo 3D system and the zebrafish model |
title_sort | innovative approaches to establish and characterize primary cultures: an ex vivo 3d system and the zebrafish model |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5312106/ https://www.ncbi.nlm.nih.gov/pubmed/27895047 http://dx.doi.org/10.1242/bio.022483 |
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