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Identification, classification, and transcription profiles of the B-type response regulator family in pear
Type-B response regulators (B-RRs) are transcription factors that function in the final step of two-component signaling systems. In model plants, B-RRs have been shown to play important roles in cytokinin signal transduction. However, the functions of B-RRs in pear have not been well studied. In thi...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5312876/ https://www.ncbi.nlm.nih.gov/pubmed/28207822 http://dx.doi.org/10.1371/journal.pone.0171523 |
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author | Ni, Junbei Bai, Songling Gao, Ling Qian, Minjie Zhong, Linbing Teng, Yuanwen |
author_facet | Ni, Junbei Bai, Songling Gao, Ling Qian, Minjie Zhong, Linbing Teng, Yuanwen |
author_sort | Ni, Junbei |
collection | PubMed |
description | Type-B response regulators (B-RRs) are transcription factors that function in the final step of two-component signaling systems. In model plants, B-RRs have been shown to play important roles in cytokinin signal transduction. However, the functions of B-RRs in pear have not been well studied. In this report, we conducted a genome-wide analysis and identified 11 putative genes encoding B-PpRR proteins based on the published genome sequence of Pyrus bretschneideri. A phylogenetic tree of the B-PpRR family was constructed, and the motif distribution, chromosome localization, and gene structure of B-PpRR family genes were determined. Gene transcript profiles, which were determined from transcriptome data, indicated that B-PpRR genes potentially function during pear fruit development, bud dormancy, and light/hormone-induced anthocyanin accumulation. Treatment of the fruitlets of ‘Cuiguan’ pear (Pyrus pyrifolia), which never accumulates anthocyanin, with the cytokinin N-(2-chloro-4-pyridyl)- N′-phenylurea (CPPU) clearly induced anthocyanin accumulation. Anthocyanins accumulated in the skin of fruitlets by 16 days after CPPU treatment, along with the significant activation of most anthocyanin biosynthetic genes. Analyses of B-PpRR transcript levels suggested that B-PpRR genes mediated this accumulation of anthocyanins. These findings enrich our understanding of the function of B-PpRR genes in the physiological processes of pear. |
format | Online Article Text |
id | pubmed-5312876 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-53128762017-03-03 Identification, classification, and transcription profiles of the B-type response regulator family in pear Ni, Junbei Bai, Songling Gao, Ling Qian, Minjie Zhong, Linbing Teng, Yuanwen PLoS One Research Article Type-B response regulators (B-RRs) are transcription factors that function in the final step of two-component signaling systems. In model plants, B-RRs have been shown to play important roles in cytokinin signal transduction. However, the functions of B-RRs in pear have not been well studied. In this report, we conducted a genome-wide analysis and identified 11 putative genes encoding B-PpRR proteins based on the published genome sequence of Pyrus bretschneideri. A phylogenetic tree of the B-PpRR family was constructed, and the motif distribution, chromosome localization, and gene structure of B-PpRR family genes were determined. Gene transcript profiles, which were determined from transcriptome data, indicated that B-PpRR genes potentially function during pear fruit development, bud dormancy, and light/hormone-induced anthocyanin accumulation. Treatment of the fruitlets of ‘Cuiguan’ pear (Pyrus pyrifolia), which never accumulates anthocyanin, with the cytokinin N-(2-chloro-4-pyridyl)- N′-phenylurea (CPPU) clearly induced anthocyanin accumulation. Anthocyanins accumulated in the skin of fruitlets by 16 days after CPPU treatment, along with the significant activation of most anthocyanin biosynthetic genes. Analyses of B-PpRR transcript levels suggested that B-PpRR genes mediated this accumulation of anthocyanins. These findings enrich our understanding of the function of B-PpRR genes in the physiological processes of pear. Public Library of Science 2017-02-16 /pmc/articles/PMC5312876/ /pubmed/28207822 http://dx.doi.org/10.1371/journal.pone.0171523 Text en © 2017 Ni et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Ni, Junbei Bai, Songling Gao, Ling Qian, Minjie Zhong, Linbing Teng, Yuanwen Identification, classification, and transcription profiles of the B-type response regulator family in pear |
title | Identification, classification, and transcription profiles of the B-type response regulator family in pear |
title_full | Identification, classification, and transcription profiles of the B-type response regulator family in pear |
title_fullStr | Identification, classification, and transcription profiles of the B-type response regulator family in pear |
title_full_unstemmed | Identification, classification, and transcription profiles of the B-type response regulator family in pear |
title_short | Identification, classification, and transcription profiles of the B-type response regulator family in pear |
title_sort | identification, classification, and transcription profiles of the b-type response regulator family in pear |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5312876/ https://www.ncbi.nlm.nih.gov/pubmed/28207822 http://dx.doi.org/10.1371/journal.pone.0171523 |
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