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Phosphorothioate DNA Stabilized Fluorescent Gold and Silver Nanoclusters
Unmodified single-stranded DNA has recently gained popularity for the templated synthesis of fluorescent noble metal nanoclusters (NCs). Bright, stable, and biocompatible clusters have been developed primarily through optimization of DNA sequence. However, DNA backbone modifications have not yet bee...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5312886/ https://www.ncbi.nlm.nih.gov/pubmed/28347036 http://dx.doi.org/10.3390/nano5020804 |
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author | Weadick, Daniel S. Liu, Juewen |
author_facet | Weadick, Daniel S. Liu, Juewen |
author_sort | Weadick, Daniel S. |
collection | PubMed |
description | Unmodified single-stranded DNA has recently gained popularity for the templated synthesis of fluorescent noble metal nanoclusters (NCs). Bright, stable, and biocompatible clusters have been developed primarily through optimization of DNA sequence. However, DNA backbone modifications have not yet been investigated. In this work, phosphorothioate (PS) DNAs are evaluated in the synthesis of Au and Ag nanoclusters, and are employed to successfully template a novel emitter using T(15) DNA at neutral pH. Mechanistic studies indicate a distinct UV-dependent formation mechanism that does not occur through the previously reported thymine N3. The positions of PS substitution have been optimized. This is the first reported use of a T(15) template at physiological pH for AgNCs. |
format | Online Article Text |
id | pubmed-5312886 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-53128862017-03-21 Phosphorothioate DNA Stabilized Fluorescent Gold and Silver Nanoclusters Weadick, Daniel S. Liu, Juewen Nanomaterials (Basel) Article Unmodified single-stranded DNA has recently gained popularity for the templated synthesis of fluorescent noble metal nanoclusters (NCs). Bright, stable, and biocompatible clusters have been developed primarily through optimization of DNA sequence. However, DNA backbone modifications have not yet been investigated. In this work, phosphorothioate (PS) DNAs are evaluated in the synthesis of Au and Ag nanoclusters, and are employed to successfully template a novel emitter using T(15) DNA at neutral pH. Mechanistic studies indicate a distinct UV-dependent formation mechanism that does not occur through the previously reported thymine N3. The positions of PS substitution have been optimized. This is the first reported use of a T(15) template at physiological pH for AgNCs. MDPI 2015-05-19 /pmc/articles/PMC5312886/ /pubmed/28347036 http://dx.doi.org/10.3390/nano5020804 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Weadick, Daniel S. Liu, Juewen Phosphorothioate DNA Stabilized Fluorescent Gold and Silver Nanoclusters |
title | Phosphorothioate DNA Stabilized Fluorescent Gold and Silver Nanoclusters |
title_full | Phosphorothioate DNA Stabilized Fluorescent Gold and Silver Nanoclusters |
title_fullStr | Phosphorothioate DNA Stabilized Fluorescent Gold and Silver Nanoclusters |
title_full_unstemmed | Phosphorothioate DNA Stabilized Fluorescent Gold and Silver Nanoclusters |
title_short | Phosphorothioate DNA Stabilized Fluorescent Gold and Silver Nanoclusters |
title_sort | phosphorothioate dna stabilized fluorescent gold and silver nanoclusters |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5312886/ https://www.ncbi.nlm.nih.gov/pubmed/28347036 http://dx.doi.org/10.3390/nano5020804 |
work_keys_str_mv | AT weadickdaniels phosphorothioatednastabilizedfluorescentgoldandsilvernanoclusters AT liujuewen phosphorothioatednastabilizedfluorescentgoldandsilvernanoclusters |