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Phosphorothioate DNA Stabilized Fluorescent Gold and Silver Nanoclusters

Unmodified single-stranded DNA has recently gained popularity for the templated synthesis of fluorescent noble metal nanoclusters (NCs). Bright, stable, and biocompatible clusters have been developed primarily through optimization of DNA sequence. However, DNA backbone modifications have not yet bee...

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Detalles Bibliográficos
Autores principales: Weadick, Daniel S., Liu, Juewen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5312886/
https://www.ncbi.nlm.nih.gov/pubmed/28347036
http://dx.doi.org/10.3390/nano5020804
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author Weadick, Daniel S.
Liu, Juewen
author_facet Weadick, Daniel S.
Liu, Juewen
author_sort Weadick, Daniel S.
collection PubMed
description Unmodified single-stranded DNA has recently gained popularity for the templated synthesis of fluorescent noble metal nanoclusters (NCs). Bright, stable, and biocompatible clusters have been developed primarily through optimization of DNA sequence. However, DNA backbone modifications have not yet been investigated. In this work, phosphorothioate (PS) DNAs are evaluated in the synthesis of Au and Ag nanoclusters, and are employed to successfully template a novel emitter using T(15) DNA at neutral pH. Mechanistic studies indicate a distinct UV-dependent formation mechanism that does not occur through the previously reported thymine N3. The positions of PS substitution have been optimized. This is the first reported use of a T(15) template at physiological pH for AgNCs.
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spelling pubmed-53128862017-03-21 Phosphorothioate DNA Stabilized Fluorescent Gold and Silver Nanoclusters Weadick, Daniel S. Liu, Juewen Nanomaterials (Basel) Article Unmodified single-stranded DNA has recently gained popularity for the templated synthesis of fluorescent noble metal nanoclusters (NCs). Bright, stable, and biocompatible clusters have been developed primarily through optimization of DNA sequence. However, DNA backbone modifications have not yet been investigated. In this work, phosphorothioate (PS) DNAs are evaluated in the synthesis of Au and Ag nanoclusters, and are employed to successfully template a novel emitter using T(15) DNA at neutral pH. Mechanistic studies indicate a distinct UV-dependent formation mechanism that does not occur through the previously reported thymine N3. The positions of PS substitution have been optimized. This is the first reported use of a T(15) template at physiological pH for AgNCs. MDPI 2015-05-19 /pmc/articles/PMC5312886/ /pubmed/28347036 http://dx.doi.org/10.3390/nano5020804 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Weadick, Daniel S.
Liu, Juewen
Phosphorothioate DNA Stabilized Fluorescent Gold and Silver Nanoclusters
title Phosphorothioate DNA Stabilized Fluorescent Gold and Silver Nanoclusters
title_full Phosphorothioate DNA Stabilized Fluorescent Gold and Silver Nanoclusters
title_fullStr Phosphorothioate DNA Stabilized Fluorescent Gold and Silver Nanoclusters
title_full_unstemmed Phosphorothioate DNA Stabilized Fluorescent Gold and Silver Nanoclusters
title_short Phosphorothioate DNA Stabilized Fluorescent Gold and Silver Nanoclusters
title_sort phosphorothioate dna stabilized fluorescent gold and silver nanoclusters
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5312886/
https://www.ncbi.nlm.nih.gov/pubmed/28347036
http://dx.doi.org/10.3390/nano5020804
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