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Examination of Single-Walled Carbon Nanotubes Uptake and Toxicity from Dietary Exposure: Tracking Movement and Impacts in the Gastrointestinal System

Previous studies indicate that exposure of fish to pristine single-walled carbon nanotubes (SWCNTs) by oral gavage, causes no overt toxicity, and no appreciable absorption has been observed. However, in the environment, SWCNTs are likely to be present in dietary sources, which may result in differen...

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Autores principales: Bisesi, Joseph H., Ngo, Thuy, Ponnavolu, Satvika, Liu, Keira, Lavelle, Candice M., Afrooz, A.R.M. Nabiul, Saleh, Navid B., Ferguson, P. Lee, Denslow, Nancy D., Sabo-Attwood, Tara
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5312889/
https://www.ncbi.nlm.nih.gov/pubmed/28347052
http://dx.doi.org/10.3390/nano5021066
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author Bisesi, Joseph H.
Ngo, Thuy
Ponnavolu, Satvika
Liu, Keira
Lavelle, Candice M.
Afrooz, A.R.M. Nabiul
Saleh, Navid B.
Ferguson, P. Lee
Denslow, Nancy D.
Sabo-Attwood, Tara
author_facet Bisesi, Joseph H.
Ngo, Thuy
Ponnavolu, Satvika
Liu, Keira
Lavelle, Candice M.
Afrooz, A.R.M. Nabiul
Saleh, Navid B.
Ferguson, P. Lee
Denslow, Nancy D.
Sabo-Attwood, Tara
author_sort Bisesi, Joseph H.
collection PubMed
description Previous studies indicate that exposure of fish to pristine single-walled carbon nanotubes (SWCNTs) by oral gavage, causes no overt toxicity, and no appreciable absorption has been observed. However, in the environment, SWCNTs are likely to be present in dietary sources, which may result in differential impacts on uptake and biological effects. Additionally, the potential of these materials to sorb nutrients (proteins, carbohydrates, and lipids) while present in the gastrointestinal (GI) tract may lead to nutrient depletion conditions that impact processes such as growth and reproduction. To test this phenomenon, fathead minnows were fed a commercial diet either with or without SWCNTs for 96 h. Tracking and quantification of SWCNTs using near-infrared fluorescence (NIRF) imaging during feeding studies showed the presence of food does not facilitate transport of SWCNTs across the intestinal epithelia. Targeting genes shown to be responsive to nutrient depletion (peptide transporters, peptide hormones, and lipases) indicated that pept2, a peptide transporter, and cck, a peptide hormone, showed differential mRNA expression by 96 h, a response that may be indicative of nutrient limitation. The results of the current study increase our understanding of the movement of SWCNTs through the GI tract, while the changes in nutrient processing genes highlight a novel mechanism of sublethal toxicity in aquatic organisms.
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spelling pubmed-53128892017-03-21 Examination of Single-Walled Carbon Nanotubes Uptake and Toxicity from Dietary Exposure: Tracking Movement and Impacts in the Gastrointestinal System Bisesi, Joseph H. Ngo, Thuy Ponnavolu, Satvika Liu, Keira Lavelle, Candice M. Afrooz, A.R.M. Nabiul Saleh, Navid B. Ferguson, P. Lee Denslow, Nancy D. Sabo-Attwood, Tara Nanomaterials (Basel) Article Previous studies indicate that exposure of fish to pristine single-walled carbon nanotubes (SWCNTs) by oral gavage, causes no overt toxicity, and no appreciable absorption has been observed. However, in the environment, SWCNTs are likely to be present in dietary sources, which may result in differential impacts on uptake and biological effects. Additionally, the potential of these materials to sorb nutrients (proteins, carbohydrates, and lipids) while present in the gastrointestinal (GI) tract may lead to nutrient depletion conditions that impact processes such as growth and reproduction. To test this phenomenon, fathead minnows were fed a commercial diet either with or without SWCNTs for 96 h. Tracking and quantification of SWCNTs using near-infrared fluorescence (NIRF) imaging during feeding studies showed the presence of food does not facilitate transport of SWCNTs across the intestinal epithelia. Targeting genes shown to be responsive to nutrient depletion (peptide transporters, peptide hormones, and lipases) indicated that pept2, a peptide transporter, and cck, a peptide hormone, showed differential mRNA expression by 96 h, a response that may be indicative of nutrient limitation. The results of the current study increase our understanding of the movement of SWCNTs through the GI tract, while the changes in nutrient processing genes highlight a novel mechanism of sublethal toxicity in aquatic organisms. MDPI 2015-06-12 /pmc/articles/PMC5312889/ /pubmed/28347052 http://dx.doi.org/10.3390/nano5021066 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bisesi, Joseph H.
Ngo, Thuy
Ponnavolu, Satvika
Liu, Keira
Lavelle, Candice M.
Afrooz, A.R.M. Nabiul
Saleh, Navid B.
Ferguson, P. Lee
Denslow, Nancy D.
Sabo-Attwood, Tara
Examination of Single-Walled Carbon Nanotubes Uptake and Toxicity from Dietary Exposure: Tracking Movement and Impacts in the Gastrointestinal System
title Examination of Single-Walled Carbon Nanotubes Uptake and Toxicity from Dietary Exposure: Tracking Movement and Impacts in the Gastrointestinal System
title_full Examination of Single-Walled Carbon Nanotubes Uptake and Toxicity from Dietary Exposure: Tracking Movement and Impacts in the Gastrointestinal System
title_fullStr Examination of Single-Walled Carbon Nanotubes Uptake and Toxicity from Dietary Exposure: Tracking Movement and Impacts in the Gastrointestinal System
title_full_unstemmed Examination of Single-Walled Carbon Nanotubes Uptake and Toxicity from Dietary Exposure: Tracking Movement and Impacts in the Gastrointestinal System
title_short Examination of Single-Walled Carbon Nanotubes Uptake and Toxicity from Dietary Exposure: Tracking Movement and Impacts in the Gastrointestinal System
title_sort examination of single-walled carbon nanotubes uptake and toxicity from dietary exposure: tracking movement and impacts in the gastrointestinal system
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5312889/
https://www.ncbi.nlm.nih.gov/pubmed/28347052
http://dx.doi.org/10.3390/nano5021066
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