Targeting of Apoptotic Cells Using Functionalized Fe(2)O(3) Nanoparticles

Fe(2)O(3) nanoparticles (NPs) have been synthesized and functionalized with SiO(2) and -NH(2) group, respectively. Conjugation to fluorescently-labeled poly-caspase inhibitor (SR-FLIVO) has been carried out for better cellular uptake studies of apoptosis arising from brain focal cerebral ischemia. H...

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Detalles Bibliográficos
Autores principales: Mekawy, Moataz, Saito, Atsushi, Shimizu, Hiroaki, Tominaga, Teiji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5312913/
https://www.ncbi.nlm.nih.gov/pubmed/28347041
http://dx.doi.org/10.3390/nano5020874
Descripción
Sumario:Fe(2)O(3) nanoparticles (NPs) have been synthesized and functionalized with SiO(2) and -NH(2) group, respectively. Conjugation to fluorescently-labeled poly-caspase inhibitor (SR-FLIVO) has been carried out for better cellular uptake studies of apoptosis arising from brain focal cerebral ischemia. Highest conjugation affinity to SR-FLIVO was found to be ca. 80% for Fe(2)O(3)-SiO-NH(2) functionalized nanoparticles (FNPs). Tracking of SR-FLIVO conjugated functionalized nanoparticles (SR-FLIVO-FNPs) in vivo and in vitro has been carried out and detected using microscopic techniques after histochemical staining methods. Experimental results revealed that SR-FLIVO-FNPs probe could passively cross the blood brain barrier (BBB) and accumulated within the apoptotic cell. Optimization of SR-FLIVO-FNPs probe can effectively promise to open a new era for intracellular drug delivery and brain diagnosis.

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