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Lipidomic approach for stratification of acute myeloid leukemia patients
The pathogenesis and progression of many tumors, including hematologic malignancies is highly dependent on enhanced lipogenesis. De novo fatty-acid synthesis permits accelerated proliferation of tumor cells by providing membrane components but these may also alter physicochemical properties of lipid...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5313223/ https://www.ncbi.nlm.nih.gov/pubmed/28207743 http://dx.doi.org/10.1371/journal.pone.0168781 |
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author | Stefanko, Adam Thiede, Christian Ehninger, Gerhard Simons, Kai Grzybek, Michal |
author_facet | Stefanko, Adam Thiede, Christian Ehninger, Gerhard Simons, Kai Grzybek, Michal |
author_sort | Stefanko, Adam |
collection | PubMed |
description | The pathogenesis and progression of many tumors, including hematologic malignancies is highly dependent on enhanced lipogenesis. De novo fatty-acid synthesis permits accelerated proliferation of tumor cells by providing membrane components but these may also alter physicochemical properties of lipid bilayers, which can impact signaling or even increase drug resistance in cancer cells. Cancer type-specific lipid profiles would permit us to monitor and interpret actual effects of lipid changes, potential fingerprints of individual tumors to be explored as diagnostic markers. We have used the shotgun MS approach to identify lipid patterns in different types of acute myeloid leukemia (AML) patients that either show no karyotype change or belong to t(8;21) or inv16 types. Differences in lipidomes of t(8;21) and inv(16) patients, as compared to AML patients without karyotype change, presented mostly as substantial modulation of ceramide/sphingolipid synthesis. Furthermore, between the t(8;21) and all other patients we observed significant changes in physicochemical membrane properties. These were related to a marked alteration in lipid saturation levels. The discovered differences in lipid profiles of various AML types improve our understanding of the pathobiochemical pathways involved and may serve in the development of diagnostic tools. |
format | Online Article Text |
id | pubmed-5313223 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-53132232017-03-03 Lipidomic approach for stratification of acute myeloid leukemia patients Stefanko, Adam Thiede, Christian Ehninger, Gerhard Simons, Kai Grzybek, Michal PLoS One Research Article The pathogenesis and progression of many tumors, including hematologic malignancies is highly dependent on enhanced lipogenesis. De novo fatty-acid synthesis permits accelerated proliferation of tumor cells by providing membrane components but these may also alter physicochemical properties of lipid bilayers, which can impact signaling or even increase drug resistance in cancer cells. Cancer type-specific lipid profiles would permit us to monitor and interpret actual effects of lipid changes, potential fingerprints of individual tumors to be explored as diagnostic markers. We have used the shotgun MS approach to identify lipid patterns in different types of acute myeloid leukemia (AML) patients that either show no karyotype change or belong to t(8;21) or inv16 types. Differences in lipidomes of t(8;21) and inv(16) patients, as compared to AML patients without karyotype change, presented mostly as substantial modulation of ceramide/sphingolipid synthesis. Furthermore, between the t(8;21) and all other patients we observed significant changes in physicochemical membrane properties. These were related to a marked alteration in lipid saturation levels. The discovered differences in lipid profiles of various AML types improve our understanding of the pathobiochemical pathways involved and may serve in the development of diagnostic tools. Public Library of Science 2017-02-16 /pmc/articles/PMC5313223/ /pubmed/28207743 http://dx.doi.org/10.1371/journal.pone.0168781 Text en © 2017 Stefanko et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Stefanko, Adam Thiede, Christian Ehninger, Gerhard Simons, Kai Grzybek, Michal Lipidomic approach for stratification of acute myeloid leukemia patients |
title | Lipidomic approach for stratification of acute myeloid leukemia patients |
title_full | Lipidomic approach for stratification of acute myeloid leukemia patients |
title_fullStr | Lipidomic approach for stratification of acute myeloid leukemia patients |
title_full_unstemmed | Lipidomic approach for stratification of acute myeloid leukemia patients |
title_short | Lipidomic approach for stratification of acute myeloid leukemia patients |
title_sort | lipidomic approach for stratification of acute myeloid leukemia patients |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5313223/ https://www.ncbi.nlm.nih.gov/pubmed/28207743 http://dx.doi.org/10.1371/journal.pone.0168781 |
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