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Lipidomic approach for stratification of acute myeloid leukemia patients

The pathogenesis and progression of many tumors, including hematologic malignancies is highly dependent on enhanced lipogenesis. De novo fatty-acid synthesis permits accelerated proliferation of tumor cells by providing membrane components but these may also alter physicochemical properties of lipid...

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Autores principales: Stefanko, Adam, Thiede, Christian, Ehninger, Gerhard, Simons, Kai, Grzybek, Michal
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5313223/
https://www.ncbi.nlm.nih.gov/pubmed/28207743
http://dx.doi.org/10.1371/journal.pone.0168781
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author Stefanko, Adam
Thiede, Christian
Ehninger, Gerhard
Simons, Kai
Grzybek, Michal
author_facet Stefanko, Adam
Thiede, Christian
Ehninger, Gerhard
Simons, Kai
Grzybek, Michal
author_sort Stefanko, Adam
collection PubMed
description The pathogenesis and progression of many tumors, including hematologic malignancies is highly dependent on enhanced lipogenesis. De novo fatty-acid synthesis permits accelerated proliferation of tumor cells by providing membrane components but these may also alter physicochemical properties of lipid bilayers, which can impact signaling or even increase drug resistance in cancer cells. Cancer type-specific lipid profiles would permit us to monitor and interpret actual effects of lipid changes, potential fingerprints of individual tumors to be explored as diagnostic markers. We have used the shotgun MS approach to identify lipid patterns in different types of acute myeloid leukemia (AML) patients that either show no karyotype change or belong to t(8;21) or inv16 types. Differences in lipidomes of t(8;21) and inv(16) patients, as compared to AML patients without karyotype change, presented mostly as substantial modulation of ceramide/sphingolipid synthesis. Furthermore, between the t(8;21) and all other patients we observed significant changes in physicochemical membrane properties. These were related to a marked alteration in lipid saturation levels. The discovered differences in lipid profiles of various AML types improve our understanding of the pathobiochemical pathways involved and may serve in the development of diagnostic tools.
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spelling pubmed-53132232017-03-03 Lipidomic approach for stratification of acute myeloid leukemia patients Stefanko, Adam Thiede, Christian Ehninger, Gerhard Simons, Kai Grzybek, Michal PLoS One Research Article The pathogenesis and progression of many tumors, including hematologic malignancies is highly dependent on enhanced lipogenesis. De novo fatty-acid synthesis permits accelerated proliferation of tumor cells by providing membrane components but these may also alter physicochemical properties of lipid bilayers, which can impact signaling or even increase drug resistance in cancer cells. Cancer type-specific lipid profiles would permit us to monitor and interpret actual effects of lipid changes, potential fingerprints of individual tumors to be explored as diagnostic markers. We have used the shotgun MS approach to identify lipid patterns in different types of acute myeloid leukemia (AML) patients that either show no karyotype change or belong to t(8;21) or inv16 types. Differences in lipidomes of t(8;21) and inv(16) patients, as compared to AML patients without karyotype change, presented mostly as substantial modulation of ceramide/sphingolipid synthesis. Furthermore, between the t(8;21) and all other patients we observed significant changes in physicochemical membrane properties. These were related to a marked alteration in lipid saturation levels. The discovered differences in lipid profiles of various AML types improve our understanding of the pathobiochemical pathways involved and may serve in the development of diagnostic tools. Public Library of Science 2017-02-16 /pmc/articles/PMC5313223/ /pubmed/28207743 http://dx.doi.org/10.1371/journal.pone.0168781 Text en © 2017 Stefanko et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Stefanko, Adam
Thiede, Christian
Ehninger, Gerhard
Simons, Kai
Grzybek, Michal
Lipidomic approach for stratification of acute myeloid leukemia patients
title Lipidomic approach for stratification of acute myeloid leukemia patients
title_full Lipidomic approach for stratification of acute myeloid leukemia patients
title_fullStr Lipidomic approach for stratification of acute myeloid leukemia patients
title_full_unstemmed Lipidomic approach for stratification of acute myeloid leukemia patients
title_short Lipidomic approach for stratification of acute myeloid leukemia patients
title_sort lipidomic approach for stratification of acute myeloid leukemia patients
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5313223/
https://www.ncbi.nlm.nih.gov/pubmed/28207743
http://dx.doi.org/10.1371/journal.pone.0168781
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