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Methane Production in Dairy Cows Correlates with Rumen Methanogenic and Bacterial Community Structure
Methane (CH(4)) is produced as an end product from feed fermentation in the rumen. Yield of CH(4) varies between individuals despite identical feeding conditions. To get a better understanding of factors behind the individual variation, 73 dairy cows given the same feed but differing in CH(4) emissi...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2017
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5313486/ https://www.ncbi.nlm.nih.gov/pubmed/28261182 http://dx.doi.org/10.3389/fmicb.2017.00226 |
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author | Danielsson, Rebecca Dicksved, Johan Sun, Li Gonda, Horacio Müller, Bettina Schnürer, Anna Bertilsson, Jan |
author_facet | Danielsson, Rebecca Dicksved, Johan Sun, Li Gonda, Horacio Müller, Bettina Schnürer, Anna Bertilsson, Jan |
author_sort | Danielsson, Rebecca |
collection | PubMed |
description | Methane (CH(4)) is produced as an end product from feed fermentation in the rumen. Yield of CH(4) varies between individuals despite identical feeding conditions. To get a better understanding of factors behind the individual variation, 73 dairy cows given the same feed but differing in CH(4) emissions were investigated with focus on fiber digestion, fermentation end products and bacterial and archaeal composition. In total 21 cows (12 Holstein, 9 Swedish Red) identified as persistent low, medium or high CH(4) emitters over a 3 month period were furthermore chosen for analysis of microbial community structure in rumen fluid. This was assessed by sequencing the V4 region of 16S rRNA gene and by quantitative qPCR of targeted Methanobrevibacter groups. The results showed a positive correlation between low CH(4) emitters and higher abundance of Methanobrevibacter ruminantium clade. Principal coordinate analysis (PCoA) on operational taxonomic unit (OTU) level of bacteria showed two distinct clusters (P < 0.01) that were related to CH(4) production. One cluster was associated with low CH(4) production (referred to as cluster L) whereas the other cluster was associated with high CH(4) production (cluster H) and the medium emitters occurred in both clusters. The differences between clusters were primarily linked to differential abundances of certain OTUs belonging to Prevotella. Moreover, several OTUs belonging to the family Succinivibrionaceae were dominant in samples belonging to cluster L. Fermentation pattern of volatile fatty acids showed that proportion of propionate was higher in cluster L, while proportion of butyrate was higher in cluster H. No difference was found in milk production or organic matter digestibility between cows. Cows in cluster L had lower CH(4)/kg energy corrected milk (ECM) compared to cows in cluster H, 8.3 compared to 9.7 g CH(4)/kg ECM, showing that low CH(4) cows utilized the feed more efficient for milk production which might indicate a more efficient microbial population or host genetic differences that is reflected in bacterial and archaeal (or methanogens) populations. |
format | Online Article Text |
id | pubmed-5313486 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-53134862017-03-03 Methane Production in Dairy Cows Correlates with Rumen Methanogenic and Bacterial Community Structure Danielsson, Rebecca Dicksved, Johan Sun, Li Gonda, Horacio Müller, Bettina Schnürer, Anna Bertilsson, Jan Front Microbiol Microbiology Methane (CH(4)) is produced as an end product from feed fermentation in the rumen. Yield of CH(4) varies between individuals despite identical feeding conditions. To get a better understanding of factors behind the individual variation, 73 dairy cows given the same feed but differing in CH(4) emissions were investigated with focus on fiber digestion, fermentation end products and bacterial and archaeal composition. In total 21 cows (12 Holstein, 9 Swedish Red) identified as persistent low, medium or high CH(4) emitters over a 3 month period were furthermore chosen for analysis of microbial community structure in rumen fluid. This was assessed by sequencing the V4 region of 16S rRNA gene and by quantitative qPCR of targeted Methanobrevibacter groups. The results showed a positive correlation between low CH(4) emitters and higher abundance of Methanobrevibacter ruminantium clade. Principal coordinate analysis (PCoA) on operational taxonomic unit (OTU) level of bacteria showed two distinct clusters (P < 0.01) that were related to CH(4) production. One cluster was associated with low CH(4) production (referred to as cluster L) whereas the other cluster was associated with high CH(4) production (cluster H) and the medium emitters occurred in both clusters. The differences between clusters were primarily linked to differential abundances of certain OTUs belonging to Prevotella. Moreover, several OTUs belonging to the family Succinivibrionaceae were dominant in samples belonging to cluster L. Fermentation pattern of volatile fatty acids showed that proportion of propionate was higher in cluster L, while proportion of butyrate was higher in cluster H. No difference was found in milk production or organic matter digestibility between cows. Cows in cluster L had lower CH(4)/kg energy corrected milk (ECM) compared to cows in cluster H, 8.3 compared to 9.7 g CH(4)/kg ECM, showing that low CH(4) cows utilized the feed more efficient for milk production which might indicate a more efficient microbial population or host genetic differences that is reflected in bacterial and archaeal (or methanogens) populations. Frontiers Media S.A. 2017-02-17 /pmc/articles/PMC5313486/ /pubmed/28261182 http://dx.doi.org/10.3389/fmicb.2017.00226 Text en Copyright © 2017 Danielsson, Dicksved, Sun, Gonda, Müller, Schnürer and Bertilsson. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Danielsson, Rebecca Dicksved, Johan Sun, Li Gonda, Horacio Müller, Bettina Schnürer, Anna Bertilsson, Jan Methane Production in Dairy Cows Correlates with Rumen Methanogenic and Bacterial Community Structure |
title | Methane Production in Dairy Cows Correlates with Rumen Methanogenic and Bacterial Community Structure |
title_full | Methane Production in Dairy Cows Correlates with Rumen Methanogenic and Bacterial Community Structure |
title_fullStr | Methane Production in Dairy Cows Correlates with Rumen Methanogenic and Bacterial Community Structure |
title_full_unstemmed | Methane Production in Dairy Cows Correlates with Rumen Methanogenic and Bacterial Community Structure |
title_short | Methane Production in Dairy Cows Correlates with Rumen Methanogenic and Bacterial Community Structure |
title_sort | methane production in dairy cows correlates with rumen methanogenic and bacterial community structure |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5313486/ https://www.ncbi.nlm.nih.gov/pubmed/28261182 http://dx.doi.org/10.3389/fmicb.2017.00226 |
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