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Rabconnectin-3α is required for the morphological maturation of GnRH neurons and kisspeptin responsiveness
A few hundred hypothalamic neurons form a complex network that controls reproduction in mammals by secreting gonadotropin-releasing hormone (GnRH). Timely postnatal changes in GnRH secretion are essential for pubertal onset. During the juvenile period, GnRH neurons undergo morphological remodeling,...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5314327/ https://www.ncbi.nlm.nih.gov/pubmed/28209974 http://dx.doi.org/10.1038/srep42463 |
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author | Tata, Brooke K. Harbulot, Carole Csaba, Zsolt Peineau, Stéphane Jacquier, Sandrine de Roux, Nicolas |
author_facet | Tata, Brooke K. Harbulot, Carole Csaba, Zsolt Peineau, Stéphane Jacquier, Sandrine de Roux, Nicolas |
author_sort | Tata, Brooke K. |
collection | PubMed |
description | A few hundred hypothalamic neurons form a complex network that controls reproduction in mammals by secreting gonadotropin-releasing hormone (GnRH). Timely postnatal changes in GnRH secretion are essential for pubertal onset. During the juvenile period, GnRH neurons undergo morphological remodeling, concomitantly achieving an increased responsiveness to kisspeptin, the main secretagogue of GnRH. However, the link between GnRH neuron activity and their morphology remains unknown. Here, we show that brain expression levels of Dmxl2, which encodes the vesicular protein rabconnectin-3α, determine the capacity of GnRH neurons to be activated by kisspeptin and estradiol. We also demonstrate that Dmxl2 expression levels control the pruning of GnRH dendrites, highlighting an unexpected role for a vesicular protein in the maturation of GnRH neuronal network. This effect is mediated by rabconnectin-3α in neurons or glial cells afferent to GnRH neurons. The widespread expression of Dmxl2 in several brain areas raises the intriguing hypothesis that rabconnectin-3α could be involved in the maturation of other neuronal populations. |
format | Online Article Text |
id | pubmed-5314327 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-53143272017-02-23 Rabconnectin-3α is required for the morphological maturation of GnRH neurons and kisspeptin responsiveness Tata, Brooke K. Harbulot, Carole Csaba, Zsolt Peineau, Stéphane Jacquier, Sandrine de Roux, Nicolas Sci Rep Article A few hundred hypothalamic neurons form a complex network that controls reproduction in mammals by secreting gonadotropin-releasing hormone (GnRH). Timely postnatal changes in GnRH secretion are essential for pubertal onset. During the juvenile period, GnRH neurons undergo morphological remodeling, concomitantly achieving an increased responsiveness to kisspeptin, the main secretagogue of GnRH. However, the link between GnRH neuron activity and their morphology remains unknown. Here, we show that brain expression levels of Dmxl2, which encodes the vesicular protein rabconnectin-3α, determine the capacity of GnRH neurons to be activated by kisspeptin and estradiol. We also demonstrate that Dmxl2 expression levels control the pruning of GnRH dendrites, highlighting an unexpected role for a vesicular protein in the maturation of GnRH neuronal network. This effect is mediated by rabconnectin-3α in neurons or glial cells afferent to GnRH neurons. The widespread expression of Dmxl2 in several brain areas raises the intriguing hypothesis that rabconnectin-3α could be involved in the maturation of other neuronal populations. Nature Publishing Group 2017-02-17 /pmc/articles/PMC5314327/ /pubmed/28209974 http://dx.doi.org/10.1038/srep42463 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Tata, Brooke K. Harbulot, Carole Csaba, Zsolt Peineau, Stéphane Jacquier, Sandrine de Roux, Nicolas Rabconnectin-3α is required for the morphological maturation of GnRH neurons and kisspeptin responsiveness |
title | Rabconnectin-3α is required for the morphological maturation of GnRH neurons and kisspeptin responsiveness |
title_full | Rabconnectin-3α is required for the morphological maturation of GnRH neurons and kisspeptin responsiveness |
title_fullStr | Rabconnectin-3α is required for the morphological maturation of GnRH neurons and kisspeptin responsiveness |
title_full_unstemmed | Rabconnectin-3α is required for the morphological maturation of GnRH neurons and kisspeptin responsiveness |
title_short | Rabconnectin-3α is required for the morphological maturation of GnRH neurons and kisspeptin responsiveness |
title_sort | rabconnectin-3α is required for the morphological maturation of gnrh neurons and kisspeptin responsiveness |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5314327/ https://www.ncbi.nlm.nih.gov/pubmed/28209974 http://dx.doi.org/10.1038/srep42463 |
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