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Integration of microRNA and mRNA expression profiles in the skin of systemic sclerosis patients
MicroRNAs (miRNAs) play important roles in the fibrosis of systemic sclerosis (SSc). However, the underlying miRNA-mRNA regulatory network is not fully understood. A systemic investigation of the role of miRNAs would be very valuable for increasing our knowledge of the pathogenesis of SSc. Here, we...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5314349/ https://www.ncbi.nlm.nih.gov/pubmed/28211533 http://dx.doi.org/10.1038/srep42899 |
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author | Zhou, Bin Zuo, Xiao Xia Li, Yi Sha Gao, Si Ming Dai, Xiao Dan Zhu, Hong Lin Luo, Hui |
author_facet | Zhou, Bin Zuo, Xiao Xia Li, Yi Sha Gao, Si Ming Dai, Xiao Dan Zhu, Hong Lin Luo, Hui |
author_sort | Zhou, Bin |
collection | PubMed |
description | MicroRNAs (miRNAs) play important roles in the fibrosis of systemic sclerosis (SSc). However, the underlying miRNA-mRNA regulatory network is not fully understood. A systemic investigation of the role of miRNAs would be very valuable for increasing our knowledge of the pathogenesis of SSc. Here, we combined miRNA and mRNA expression profiles and bioinformatics analyses and then performed validation experiments. we identified 21 miRNAs and 2698 mRNAs that were differentially expressed in SSc. Among these, 17 miRNAs and their 33 target mRNAs (55 miRNA-mRNA pairs) were involved in Toll-like receptor, transforming growth factor β and Wnt signalling pathways. Validation experiments revealed that miR-146b, miR-130b, miR-21, miR-31 and miR-34a levels were higher whereas miR-145 levels were lower in SSc skin tissues and fibroblasts, normal fibroblasts and endothelial cells that were stimulated with SSc serum. ACVR2B, FZD2, FZD5 and SOX2 levels were increased in SSc skin fibroblasts, normal fibroblasts and endothelial cells that were stimulated with SSc serum. We did not identify any negative correlations among these miRNA-mRNA pairs. miR-21 was specifically expressed at higher levels in SSc serum. Six miRNAs and 4 mRNAs appear to play important roles in the pathogenesis of SSc are worth investigating in future functional studies. |
format | Online Article Text |
id | pubmed-5314349 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-53143492017-02-23 Integration of microRNA and mRNA expression profiles in the skin of systemic sclerosis patients Zhou, Bin Zuo, Xiao Xia Li, Yi Sha Gao, Si Ming Dai, Xiao Dan Zhu, Hong Lin Luo, Hui Sci Rep Article MicroRNAs (miRNAs) play important roles in the fibrosis of systemic sclerosis (SSc). However, the underlying miRNA-mRNA regulatory network is not fully understood. A systemic investigation of the role of miRNAs would be very valuable for increasing our knowledge of the pathogenesis of SSc. Here, we combined miRNA and mRNA expression profiles and bioinformatics analyses and then performed validation experiments. we identified 21 miRNAs and 2698 mRNAs that were differentially expressed in SSc. Among these, 17 miRNAs and their 33 target mRNAs (55 miRNA-mRNA pairs) were involved in Toll-like receptor, transforming growth factor β and Wnt signalling pathways. Validation experiments revealed that miR-146b, miR-130b, miR-21, miR-31 and miR-34a levels were higher whereas miR-145 levels were lower in SSc skin tissues and fibroblasts, normal fibroblasts and endothelial cells that were stimulated with SSc serum. ACVR2B, FZD2, FZD5 and SOX2 levels were increased in SSc skin fibroblasts, normal fibroblasts and endothelial cells that were stimulated with SSc serum. We did not identify any negative correlations among these miRNA-mRNA pairs. miR-21 was specifically expressed at higher levels in SSc serum. Six miRNAs and 4 mRNAs appear to play important roles in the pathogenesis of SSc are worth investigating in future functional studies. Nature Publishing Group 2017-02-17 /pmc/articles/PMC5314349/ /pubmed/28211533 http://dx.doi.org/10.1038/srep42899 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Zhou, Bin Zuo, Xiao Xia Li, Yi Sha Gao, Si Ming Dai, Xiao Dan Zhu, Hong Lin Luo, Hui Integration of microRNA and mRNA expression profiles in the skin of systemic sclerosis patients |
title | Integration of microRNA and mRNA expression profiles in the skin of systemic sclerosis patients |
title_full | Integration of microRNA and mRNA expression profiles in the skin of systemic sclerosis patients |
title_fullStr | Integration of microRNA and mRNA expression profiles in the skin of systemic sclerosis patients |
title_full_unstemmed | Integration of microRNA and mRNA expression profiles in the skin of systemic sclerosis patients |
title_short | Integration of microRNA and mRNA expression profiles in the skin of systemic sclerosis patients |
title_sort | integration of microrna and mrna expression profiles in the skin of systemic sclerosis patients |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5314349/ https://www.ncbi.nlm.nih.gov/pubmed/28211533 http://dx.doi.org/10.1038/srep42899 |
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