Optimization of the production of knock-in alleles by CRISPR/Cas9 microinjection into the mouse zygote

Microinjection of the CRISPR/Cas9 system in zygotes is an efficient and comparatively fast method to generate genetically modified mice. So far, only few knock-in mice have been generated using this approach, and because no systematic study has been performed, parameters controlling the efficacy of...

Descripción completa

Detalles Bibliográficos
Autores principales: Raveux, Aurélien, Vandormael-Pournin, Sandrine, Cohen-Tannoudji, Michel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5314402/
https://www.ncbi.nlm.nih.gov/pubmed/28209967
http://dx.doi.org/10.1038/srep42661

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5314402/
https://www.ncbi.nlm.nih.gov/pubmed/28209967
http://dx.doi.org/10.1038/srep42661

Ejemplares similares