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Optimization of the production of knock-in alleles by CRISPR/Cas9 microinjection into the mouse zygote

Microinjection of the CRISPR/Cas9 system in zygotes is an efficient and comparatively fast method to generate genetically modified mice. So far, only few knock-in mice have been generated using this approach, and because no systematic study has been performed, parameters controlling the efficacy of...

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Detalles Bibliográficos
Autores principales:	Raveux, Aurélien, Vandormael-Pournin, Sandrine, Cohen-Tannoudji, Michel
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group 2017
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5314402/ https://www.ncbi.nlm.nih.gov/pubmed/28209967 http://dx.doi.org/10.1038/srep42661

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