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Biofilm formation of the black yeast-like fungus Exophiala dermatitidis and its susceptibility to antiinfective agents

Various fungi have the ability to colonize surfaces and to form biofilms. Fungal biofilm-associated infections are frequently refractory to targeted treatment because of resistance to antifungal drugs. One fungus that frequently colonises the respiratory tract of cystic fibrosis (CF) patients is the...

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Autores principales: Kirchhoff , Lisa, Olsowski, Maike, Zilmans, Katrin, Dittmer, Silke, Haase, Gerhard, Sedlacek, Ludwig, Steinmann, Eike, Buer, Jan, Rath, Peter-Michael, Steinmann, Joerg
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5314418/
https://www.ncbi.nlm.nih.gov/pubmed/28211475
http://dx.doi.org/10.1038/srep42886
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author Kirchhoff , Lisa
Olsowski, Maike
Zilmans, Katrin
Dittmer, Silke
Haase, Gerhard
Sedlacek, Ludwig
Steinmann, Eike
Buer, Jan
Rath, Peter-Michael
Steinmann, Joerg
author_facet Kirchhoff , Lisa
Olsowski, Maike
Zilmans, Katrin
Dittmer, Silke
Haase, Gerhard
Sedlacek, Ludwig
Steinmann, Eike
Buer, Jan
Rath, Peter-Michael
Steinmann, Joerg
author_sort Kirchhoff , Lisa
collection PubMed
description Various fungi have the ability to colonize surfaces and to form biofilms. Fungal biofilm-associated infections are frequently refractory to targeted treatment because of resistance to antifungal drugs. One fungus that frequently colonises the respiratory tract of cystic fibrosis (CF) patients is the opportunistic black yeast–like fungus Exophiala dermatitidis. We investigated the biofilm-forming ability of E. dermatitidis and its susceptibility to various antiinfective agents and natural compounds. We tested 58 E. dermatitidis isolates with a biofilm assay based on crystal violet staining. In addition, we used three isolates to examine the antibiofilm activity of voriconazole, micafungin, colistin, farnesol, and the plant derivatives 1,2,3,4,6-penta-O-galloyl-b-D-glucopyranose (PGG) and epigallocatechin-3-gallate (EGCG) with an XTT reduction assay. We analysed the effect of the agents on cell to surface adhesion, biofilm formation, and the mature biofilm. The biofilms were also investigated by confocal laser scan microscopy. We found that E. dermatitidis builds biofilm in a strain-specific manner. Invasive E. dermatitidis isolates form most biomass in biofilm. The antiinfective agents and the natural compounds exhibited poor antibiofilm activity. The greatest impact of the compounds was detected when they were added prior cell adhesion. These findings suggest that prevention may be more effective than treatment of biofilm-associated E. dermatitidis infections.
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spelling pubmed-53144182017-02-24 Biofilm formation of the black yeast-like fungus Exophiala dermatitidis and its susceptibility to antiinfective agents Kirchhoff , Lisa Olsowski, Maike Zilmans, Katrin Dittmer, Silke Haase, Gerhard Sedlacek, Ludwig Steinmann, Eike Buer, Jan Rath, Peter-Michael Steinmann, Joerg Sci Rep Article Various fungi have the ability to colonize surfaces and to form biofilms. Fungal biofilm-associated infections are frequently refractory to targeted treatment because of resistance to antifungal drugs. One fungus that frequently colonises the respiratory tract of cystic fibrosis (CF) patients is the opportunistic black yeast–like fungus Exophiala dermatitidis. We investigated the biofilm-forming ability of E. dermatitidis and its susceptibility to various antiinfective agents and natural compounds. We tested 58 E. dermatitidis isolates with a biofilm assay based on crystal violet staining. In addition, we used three isolates to examine the antibiofilm activity of voriconazole, micafungin, colistin, farnesol, and the plant derivatives 1,2,3,4,6-penta-O-galloyl-b-D-glucopyranose (PGG) and epigallocatechin-3-gallate (EGCG) with an XTT reduction assay. We analysed the effect of the agents on cell to surface adhesion, biofilm formation, and the mature biofilm. The biofilms were also investigated by confocal laser scan microscopy. We found that E. dermatitidis builds biofilm in a strain-specific manner. Invasive E. dermatitidis isolates form most biomass in biofilm. The antiinfective agents and the natural compounds exhibited poor antibiofilm activity. The greatest impact of the compounds was detected when they were added prior cell adhesion. These findings suggest that prevention may be more effective than treatment of biofilm-associated E. dermatitidis infections. Nature Publishing Group 2017-02-17 /pmc/articles/PMC5314418/ /pubmed/28211475 http://dx.doi.org/10.1038/srep42886 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Kirchhoff , Lisa
Olsowski, Maike
Zilmans, Katrin
Dittmer, Silke
Haase, Gerhard
Sedlacek, Ludwig
Steinmann, Eike
Buer, Jan
Rath, Peter-Michael
Steinmann, Joerg
Biofilm formation of the black yeast-like fungus Exophiala dermatitidis and its susceptibility to antiinfective agents
title Biofilm formation of the black yeast-like fungus Exophiala dermatitidis and its susceptibility to antiinfective agents
title_full Biofilm formation of the black yeast-like fungus Exophiala dermatitidis and its susceptibility to antiinfective agents
title_fullStr Biofilm formation of the black yeast-like fungus Exophiala dermatitidis and its susceptibility to antiinfective agents
title_full_unstemmed Biofilm formation of the black yeast-like fungus Exophiala dermatitidis and its susceptibility to antiinfective agents
title_short Biofilm formation of the black yeast-like fungus Exophiala dermatitidis and its susceptibility to antiinfective agents
title_sort biofilm formation of the black yeast-like fungus exophiala dermatitidis and its susceptibility to antiinfective agents
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5314418/
https://www.ncbi.nlm.nih.gov/pubmed/28211475
http://dx.doi.org/10.1038/srep42886
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