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Structural insights into NusG regulating transcription elongation
NusG is an essential transcription factor that plays multiple key regulatory roles in transcription elongation, termination and coupling translation and transcription. The core role of NusG is to enhance transcription elongation and RNA polymerase processivity. Here, we present the structure of Esch...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5314768/ https://www.ncbi.nlm.nih.gov/pubmed/27899640 http://dx.doi.org/10.1093/nar/gkw1159 |
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author | Liu, Bin Steitz, Thomas A. |
author_facet | Liu, Bin Steitz, Thomas A. |
author_sort | Liu, Bin |
collection | PubMed |
description | NusG is an essential transcription factor that plays multiple key regulatory roles in transcription elongation, termination and coupling translation and transcription. The core role of NusG is to enhance transcription elongation and RNA polymerase processivity. Here, we present the structure of Escherichia coli RNA polymerase complexed with NusG. The structure shows that the NusG N-terminal domain (NGN) binds at the central cleft of RNA polymerase surrounded by the β' clamp helices, the β protrusion, and the β lobe domains to close the promoter DNA binding channel and constrain the β' clamp domain, but with an orientation that is different from the one observed in the archaeal β' clamp–Spt4/5 complex. The structure also allows us to construct a reliable model of the complete NusG-associated transcription elongation complex, suggesting that the NGN domain binds at the upstream fork junction of the transcription elongation complex, similar to σ2 in the transcription initiation complex, to stabilize the junction, and therefore enhances transcription processivity. |
format | Online Article Text |
id | pubmed-5314768 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-53147682017-02-21 Structural insights into NusG regulating transcription elongation Liu, Bin Steitz, Thomas A. Nucleic Acids Res Structural Biology NusG is an essential transcription factor that plays multiple key regulatory roles in transcription elongation, termination and coupling translation and transcription. The core role of NusG is to enhance transcription elongation and RNA polymerase processivity. Here, we present the structure of Escherichia coli RNA polymerase complexed with NusG. The structure shows that the NusG N-terminal domain (NGN) binds at the central cleft of RNA polymerase surrounded by the β' clamp helices, the β protrusion, and the β lobe domains to close the promoter DNA binding channel and constrain the β' clamp domain, but with an orientation that is different from the one observed in the archaeal β' clamp–Spt4/5 complex. The structure also allows us to construct a reliable model of the complete NusG-associated transcription elongation complex, suggesting that the NGN domain binds at the upstream fork junction of the transcription elongation complex, similar to σ2 in the transcription initiation complex, to stabilize the junction, and therefore enhances transcription processivity. Oxford University Press 2017-01-25 2016-11-28 /pmc/articles/PMC5314768/ /pubmed/27899640 http://dx.doi.org/10.1093/nar/gkw1159 Text en © The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
spellingShingle | Structural Biology Liu, Bin Steitz, Thomas A. Structural insights into NusG regulating transcription elongation |
title | Structural insights into NusG regulating transcription elongation |
title_full | Structural insights into NusG regulating transcription elongation |
title_fullStr | Structural insights into NusG regulating transcription elongation |
title_full_unstemmed | Structural insights into NusG regulating transcription elongation |
title_short | Structural insights into NusG regulating transcription elongation |
title_sort | structural insights into nusg regulating transcription elongation |
topic | Structural Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5314768/ https://www.ncbi.nlm.nih.gov/pubmed/27899640 http://dx.doi.org/10.1093/nar/gkw1159 |
work_keys_str_mv | AT liubin structuralinsightsintonusgregulatingtranscriptionelongation AT steitzthomasa structuralinsightsintonusgregulatingtranscriptionelongation |