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Conservation of miRNA-mediated silencing mechanisms across 600 million years of animal evolution

Our current knowledge about the mechanisms of miRNA silencing is restricted to few lineages such as vertebrates, arthropods, nematodes and land plants. miRNA-mediated silencing in bilaterian animals is dependent on the proteins of the GW182 family. Here, we dissect the function of GW182 protein in t...

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Autores principales: Mauri, Marta, Kirchner, Marieluise, Aharoni, Reuven, Ciolli Mattioli, Camilla, van den Bruck, David, Gutkovitch, Nadya, Modepalli, Vengamanaidu, Selbach, Matthias, Moran, Yehu, Chekulaeva, Marina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2017
Materias:
RNA
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5314787/
https://www.ncbi.nlm.nih.gov/pubmed/27604873
http://dx.doi.org/10.1093/nar/gkw792
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author Mauri, Marta
Kirchner, Marieluise
Aharoni, Reuven
Ciolli Mattioli, Camilla
van den Bruck, David
Gutkovitch, Nadya
Modepalli, Vengamanaidu
Selbach, Matthias
Moran, Yehu
Chekulaeva, Marina
author_facet Mauri, Marta
Kirchner, Marieluise
Aharoni, Reuven
Ciolli Mattioli, Camilla
van den Bruck, David
Gutkovitch, Nadya
Modepalli, Vengamanaidu
Selbach, Matthias
Moran, Yehu
Chekulaeva, Marina
author_sort Mauri, Marta
collection PubMed
description Our current knowledge about the mechanisms of miRNA silencing is restricted to few lineages such as vertebrates, arthropods, nematodes and land plants. miRNA-mediated silencing in bilaterian animals is dependent on the proteins of the GW182 family. Here, we dissect the function of GW182 protein in the cnidarian Nematostella, separated by 600 million years from other Metazoa. Using cultured human cells, we show that Nematostella GW182 recruits the CCR4-NOT deadenylation complexes via its tryptophan-containing motifs, thereby inhibiting translation and promoting mRNA decay. Further, similarly to bilaterians, GW182 in Nematostella is recruited to the miRNA repression complex via interaction with Argonaute proteins, and functions downstream to repress mRNA. Thus, our work suggests that this mechanism of miRNA-mediated silencing was already active in the last common ancestor of Cnidaria and Bilateria.
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spelling pubmed-53147872017-02-21 Conservation of miRNA-mediated silencing mechanisms across 600 million years of animal evolution Mauri, Marta Kirchner, Marieluise Aharoni, Reuven Ciolli Mattioli, Camilla van den Bruck, David Gutkovitch, Nadya Modepalli, Vengamanaidu Selbach, Matthias Moran, Yehu Chekulaeva, Marina Nucleic Acids Res RNA Our current knowledge about the mechanisms of miRNA silencing is restricted to few lineages such as vertebrates, arthropods, nematodes and land plants. miRNA-mediated silencing in bilaterian animals is dependent on the proteins of the GW182 family. Here, we dissect the function of GW182 protein in the cnidarian Nematostella, separated by 600 million years from other Metazoa. Using cultured human cells, we show that Nematostella GW182 recruits the CCR4-NOT deadenylation complexes via its tryptophan-containing motifs, thereby inhibiting translation and promoting mRNA decay. Further, similarly to bilaterians, GW182 in Nematostella is recruited to the miRNA repression complex via interaction with Argonaute proteins, and functions downstream to repress mRNA. Thus, our work suggests that this mechanism of miRNA-mediated silencing was already active in the last common ancestor of Cnidaria and Bilateria. Oxford University Press 2017-01-25 2016-09-06 /pmc/articles/PMC5314787/ /pubmed/27604873 http://dx.doi.org/10.1093/nar/gkw792 Text en © The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle RNA
Mauri, Marta
Kirchner, Marieluise
Aharoni, Reuven
Ciolli Mattioli, Camilla
van den Bruck, David
Gutkovitch, Nadya
Modepalli, Vengamanaidu
Selbach, Matthias
Moran, Yehu
Chekulaeva, Marina
Conservation of miRNA-mediated silencing mechanisms across 600 million years of animal evolution
title Conservation of miRNA-mediated silencing mechanisms across 600 million years of animal evolution
title_full Conservation of miRNA-mediated silencing mechanisms across 600 million years of animal evolution
title_fullStr Conservation of miRNA-mediated silencing mechanisms across 600 million years of animal evolution
title_full_unstemmed Conservation of miRNA-mediated silencing mechanisms across 600 million years of animal evolution
title_short Conservation of miRNA-mediated silencing mechanisms across 600 million years of animal evolution
title_sort conservation of mirna-mediated silencing mechanisms across 600 million years of animal evolution
topic RNA
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5314787/
https://www.ncbi.nlm.nih.gov/pubmed/27604873
http://dx.doi.org/10.1093/nar/gkw792
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