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Ribonuclease selection for ribosome profiling
Ribosome profiling has emerged as a powerful method to assess global gene translation, but methodological and analytical challenges often lead to inconsistencies across labs and model organisms. A critical issue in ribosome profiling is nuclease treatment of ribosome–mRNA complexes, as it is importa...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5314788/ https://www.ncbi.nlm.nih.gov/pubmed/27638886 http://dx.doi.org/10.1093/nar/gkw822 |