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Exploring fungal mega-diversity: Pseudocercospora from Brazil

Although the genus Pseudocercospora has a worldwide distribution, it is especially diverse in tropical and subtropical countries. Species of this genus are associated with a wide range of plant species, including several economically relevant hosts. Preliminary studies of cercosporoid fungi from Bra...

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Autores principales: Silva, M., Barreto, R.W., Pereira, O.L., Freitas, N.M., Groenewald, J.Z., Crous, P.W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Naturalis Biodiversity Center & Centraallbureau voor Schimmelcultures 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5315286/
https://www.ncbi.nlm.nih.gov/pubmed/28232763
http://dx.doi.org/10.3767/003158516X691078
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author Silva, M.
Barreto, R.W.
Pereira, O.L.
Freitas, N.M.
Groenewald, J.Z.
Crous, P.W.
author_facet Silva, M.
Barreto, R.W.
Pereira, O.L.
Freitas, N.M.
Groenewald, J.Z.
Crous, P.W.
author_sort Silva, M.
collection PubMed
description Although the genus Pseudocercospora has a worldwide distribution, it is especially diverse in tropical and subtropical countries. Species of this genus are associated with a wide range of plant species, including several economically relevant hosts. Preliminary studies of cercosporoid fungi from Brazil allocated most taxa to Cercospora, but with the progressive refinement of the taxonomy of cercosporoid fungi, many species were relocated to or described in Pseudocercospora. Initially, species identification relied mostly on morphological features, and thus no cultures were preserved for later phylogenetic comparisons. In this study, a total of 27 Pseudocercospora spp. were collected, cultured, and subjected to a multigene analysis. Four genomic regions (LSU, ITS, tef1 and actA) were amplified and sequenced. A multigene Bayesian analysis was performed on the combined ITS, actA and tef1 sequence alignment. Our results based on DNA phylogeny, integrated with ecology, morphology and cultural characteristics revealed a rich diversity of Pseudocercospora species in Brazil. Twelve taxa were newly described, namely P. aeschynomenicola, P. diplusodonii, P. emmotunicola, P. manihotii, P. perae, P. planaltinensis, P. pothomorphes, P. sennae-multijugae, P. solani-pseudocapsicicola, P. vassobiae, P. wulffiae and P. xylopiae. Additionally, eight epitype specimens were designated, three species newly reported, and several new host records linked to known Pseudocercospora spp.
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spelling pubmed-53152862017-03-09 Exploring fungal mega-diversity: Pseudocercospora from Brazil Silva, M. Barreto, R.W. Pereira, O.L. Freitas, N.M. Groenewald, J.Z. Crous, P.W. Persoonia Research Article Although the genus Pseudocercospora has a worldwide distribution, it is especially diverse in tropical and subtropical countries. Species of this genus are associated with a wide range of plant species, including several economically relevant hosts. Preliminary studies of cercosporoid fungi from Brazil allocated most taxa to Cercospora, but with the progressive refinement of the taxonomy of cercosporoid fungi, many species were relocated to or described in Pseudocercospora. Initially, species identification relied mostly on morphological features, and thus no cultures were preserved for later phylogenetic comparisons. In this study, a total of 27 Pseudocercospora spp. were collected, cultured, and subjected to a multigene analysis. Four genomic regions (LSU, ITS, tef1 and actA) were amplified and sequenced. A multigene Bayesian analysis was performed on the combined ITS, actA and tef1 sequence alignment. Our results based on DNA phylogeny, integrated with ecology, morphology and cultural characteristics revealed a rich diversity of Pseudocercospora species in Brazil. Twelve taxa were newly described, namely P. aeschynomenicola, P. diplusodonii, P. emmotunicola, P. manihotii, P. perae, P. planaltinensis, P. pothomorphes, P. sennae-multijugae, P. solani-pseudocapsicicola, P. vassobiae, P. wulffiae and P. xylopiae. Additionally, eight epitype specimens were designated, three species newly reported, and several new host records linked to known Pseudocercospora spp. Naturalis Biodiversity Center & Centraallbureau voor Schimmelcultures 2016-02-12 2016-12 /pmc/articles/PMC5315286/ /pubmed/28232763 http://dx.doi.org/10.3767/003158516X691078 Text en © 2015-2016 Naturalis Biodiversity Center & Centraalbureau voor Schimmelcultures http://creativecommons.org/licenses/by-nc-nd/3.0/legalcode You are free to share - to copy, distribute and transmit the work, under the following conditions: Attribution: You must attribute the work in the manner specified by the author or licensor (but not in any way that suggests that they endorse you or your use of the work). Non-commercial: You may not use this work for commercial purposes. No derivative works: You may not alter, transform, or build upon this work. For any reuse or distribution, you must make clear to others the license terms of this work, which can be found at http://creativecommons.org/licenses/by-nc-nd/3.0/legalcode. Any of the above conditions can be waived if you get permission from the copyright holder. Nothing in this license impairs or restricts the author’s moral rights.
spellingShingle Research Article
Silva, M.
Barreto, R.W.
Pereira, O.L.
Freitas, N.M.
Groenewald, J.Z.
Crous, P.W.
Exploring fungal mega-diversity: Pseudocercospora from Brazil
title Exploring fungal mega-diversity: Pseudocercospora from Brazil
title_full Exploring fungal mega-diversity: Pseudocercospora from Brazil
title_fullStr Exploring fungal mega-diversity: Pseudocercospora from Brazil
title_full_unstemmed Exploring fungal mega-diversity: Pseudocercospora from Brazil
title_short Exploring fungal mega-diversity: Pseudocercospora from Brazil
title_sort exploring fungal mega-diversity: pseudocercospora from brazil
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5315286/
https://www.ncbi.nlm.nih.gov/pubmed/28232763
http://dx.doi.org/10.3767/003158516X691078
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