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Blood clot detection using magnetic nanoparticles

Deep vein thrombosis, the development of blood clots in the peripheral veins, is a very serious, life threatening condition that is prevalent in the elderly. To deliver proper treatment that enhances the survival rate, it is very important to detect thrombi early and at the point of care. We explore...

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Autores principales: Khurshid, Hafsa, Friedman, Bruce, Berwin, Brent, Shi, Yipeng, Ness, Dylan B., Weaver, John B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: AIP Publishing LLC 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5315662/
https://www.ncbi.nlm.nih.gov/pubmed/28289550
http://dx.doi.org/10.1063/1.4977073
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author Khurshid, Hafsa
Friedman, Bruce
Berwin, Brent
Shi, Yipeng
Ness, Dylan B.
Weaver, John B.
author_facet Khurshid, Hafsa
Friedman, Bruce
Berwin, Brent
Shi, Yipeng
Ness, Dylan B.
Weaver, John B.
author_sort Khurshid, Hafsa
collection PubMed
description Deep vein thrombosis, the development of blood clots in the peripheral veins, is a very serious, life threatening condition that is prevalent in the elderly. To deliver proper treatment that enhances the survival rate, it is very important to detect thrombi early and at the point of care. We explored the ability of magnetic particle spectroscopy (MSB) to detect thrombus via specific binding of aptamer functionalized magnetic nanoparticles with the blood clot. MSB uses the harmonics produced by nanoparticles in an alternating magnetic field to measure the rotational freedom and, therefore, the bound state of the nanoparticles. The nanoparticles’ relaxation time for Brownian rotation increases when bound [A.M. Rauwerdink and J. B. Weaver, Appl. Phys. Lett. 96, 1 (2010)]. The relaxation time can therefore be used to characterize the nanoparticle binding to thrombin in the blood clot. For longer relaxation times, the approach to saturation is more gradual reducing the higher harmonics and the harmonic ratio. The harmonic ratios of nanoparticles conjugated with anti-thrombin aptamers (ATP) decrease significantly over time with blood clot present in the sample medium, compared with nanoparticles without ATP. Moreover, the blood clot removed from the sample medium produced a significant MSB signal, indicating the nanoparticles are immobilized on the clot. Our results show that MSB could be a very useful non-invasive, quick tool to detect blood clots at the point of care so proper treatment can be used to reduce the risks inherent in deep vein thrombosis.
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spelling pubmed-53156622017-03-13 Blood clot detection using magnetic nanoparticles Khurshid, Hafsa Friedman, Bruce Berwin, Brent Shi, Yipeng Ness, Dylan B. Weaver, John B. AIP Adv THE 61ST MMM CONFERENCE PAPERS Deep vein thrombosis, the development of blood clots in the peripheral veins, is a very serious, life threatening condition that is prevalent in the elderly. To deliver proper treatment that enhances the survival rate, it is very important to detect thrombi early and at the point of care. We explored the ability of magnetic particle spectroscopy (MSB) to detect thrombus via specific binding of aptamer functionalized magnetic nanoparticles with the blood clot. MSB uses the harmonics produced by nanoparticles in an alternating magnetic field to measure the rotational freedom and, therefore, the bound state of the nanoparticles. The nanoparticles’ relaxation time for Brownian rotation increases when bound [A.M. Rauwerdink and J. B. Weaver, Appl. Phys. Lett. 96, 1 (2010)]. The relaxation time can therefore be used to characterize the nanoparticle binding to thrombin in the blood clot. For longer relaxation times, the approach to saturation is more gradual reducing the higher harmonics and the harmonic ratio. The harmonic ratios of nanoparticles conjugated with anti-thrombin aptamers (ATP) decrease significantly over time with blood clot present in the sample medium, compared with nanoparticles without ATP. Moreover, the blood clot removed from the sample medium produced a significant MSB signal, indicating the nanoparticles are immobilized on the clot. Our results show that MSB could be a very useful non-invasive, quick tool to detect blood clots at the point of care so proper treatment can be used to reduce the risks inherent in deep vein thrombosis. AIP Publishing LLC 2017-02-16 /pmc/articles/PMC5315662/ /pubmed/28289550 http://dx.doi.org/10.1063/1.4977073 Text en © 2017 Author(s). 2158-3226/2017/7(5)/056723/5/$0.00 All article content, except where otherwise noted, is licensed under a Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle THE 61ST MMM CONFERENCE PAPERS
Khurshid, Hafsa
Friedman, Bruce
Berwin, Brent
Shi, Yipeng
Ness, Dylan B.
Weaver, John B.
Blood clot detection using magnetic nanoparticles
title Blood clot detection using magnetic nanoparticles
title_full Blood clot detection using magnetic nanoparticles
title_fullStr Blood clot detection using magnetic nanoparticles
title_full_unstemmed Blood clot detection using magnetic nanoparticles
title_short Blood clot detection using magnetic nanoparticles
title_sort blood clot detection using magnetic nanoparticles
topic THE 61ST MMM CONFERENCE PAPERS
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5315662/
https://www.ncbi.nlm.nih.gov/pubmed/28289550
http://dx.doi.org/10.1063/1.4977073
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