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Porphyromonas gingivalis can invade periodontal ligament stem cells

BACKGROUND: Porphyromonas gingivalis is strongly associated with the development, progression, severity and recurrence of periodontitis. Periodontal ligament stem cells (PDLSCs) play an important role in the maintenance of periodontal tissue self-renewal and repair. The purpose of this study was to...

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Autores principales: Pan, Chunling, Liu, Junchao, Wang, Hongyan, Song, Jia, Tan, Lisi, Zhao, Haijiao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5316216/
https://www.ncbi.nlm.nih.gov/pubmed/28212613
http://dx.doi.org/10.1186/s12866-017-0950-5
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author Pan, Chunling
Liu, Junchao
Wang, Hongyan
Song, Jia
Tan, Lisi
Zhao, Haijiao
author_facet Pan, Chunling
Liu, Junchao
Wang, Hongyan
Song, Jia
Tan, Lisi
Zhao, Haijiao
author_sort Pan, Chunling
collection PubMed
description BACKGROUND: Porphyromonas gingivalis is strongly associated with the development, progression, severity and recurrence of periodontitis. Periodontal ligament stem cells (PDLSCs) play an important role in the maintenance of periodontal tissue self-renewal and repair. The purpose of this study was to investigate the ability of P. gingivalis to infect PDLSCs using an in vitro monolayer model. METHODS: We separated and cultured primary PDLSCs using the tissue block with limiting dilution method. The efficiency of P. gingivalis (ATCC 33277) infection of PDLSCs was measured using agar plate culture and quantitative polymerase chain reaction (q-PCR) methods. PDLSCs infected with P. gingivalis were also observed by transmission electron microscopy. RESULTS: We assessed stem cell properties including cell morphology, clone formation, growth activity, cell surface antigens and multiple differentiation capacity. The infection rates of P. gingivalis in PDLSC at MOIs of 50, 100, 200, and 500 were 5.83%, 8.12%, 7.77% and 7.53% according to the agar plate culture method. By q-PCR, the efficiencies of P. gingivalis infection of PDLSCs at MOIs of 50, 100, 200, and 500 were 6.74%, 10.56%, 10.36% and 9.78%, respectively. Overall, the infection efficiency based on q-PCR was higher than that according to agar plate culture. Using transmission electron microscopy, we verified that P. gingivalis (ATCC 33277) could infect and invade PDLSCs after 2 h of incubation, and endocytic vacuoles were not found surrounding the internalized bacteria. CONCLUSIONS: In conclusion, our data demonstrate that P. gingivalis can invade PDLSCs. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12866-017-0950-5) contains supplementary material, which is available to authorized users.
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spelling pubmed-53162162017-02-24 Porphyromonas gingivalis can invade periodontal ligament stem cells Pan, Chunling Liu, Junchao Wang, Hongyan Song, Jia Tan, Lisi Zhao, Haijiao BMC Microbiol Research Article BACKGROUND: Porphyromonas gingivalis is strongly associated with the development, progression, severity and recurrence of periodontitis. Periodontal ligament stem cells (PDLSCs) play an important role in the maintenance of periodontal tissue self-renewal and repair. The purpose of this study was to investigate the ability of P. gingivalis to infect PDLSCs using an in vitro monolayer model. METHODS: We separated and cultured primary PDLSCs using the tissue block with limiting dilution method. The efficiency of P. gingivalis (ATCC 33277) infection of PDLSCs was measured using agar plate culture and quantitative polymerase chain reaction (q-PCR) methods. PDLSCs infected with P. gingivalis were also observed by transmission electron microscopy. RESULTS: We assessed stem cell properties including cell morphology, clone formation, growth activity, cell surface antigens and multiple differentiation capacity. The infection rates of P. gingivalis in PDLSC at MOIs of 50, 100, 200, and 500 were 5.83%, 8.12%, 7.77% and 7.53% according to the agar plate culture method. By q-PCR, the efficiencies of P. gingivalis infection of PDLSCs at MOIs of 50, 100, 200, and 500 were 6.74%, 10.56%, 10.36% and 9.78%, respectively. Overall, the infection efficiency based on q-PCR was higher than that according to agar plate culture. Using transmission electron microscopy, we verified that P. gingivalis (ATCC 33277) could infect and invade PDLSCs after 2 h of incubation, and endocytic vacuoles were not found surrounding the internalized bacteria. CONCLUSIONS: In conclusion, our data demonstrate that P. gingivalis can invade PDLSCs. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12866-017-0950-5) contains supplementary material, which is available to authorized users. BioMed Central 2017-02-17 /pmc/articles/PMC5316216/ /pubmed/28212613 http://dx.doi.org/10.1186/s12866-017-0950-5 Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Pan, Chunling
Liu, Junchao
Wang, Hongyan
Song, Jia
Tan, Lisi
Zhao, Haijiao
Porphyromonas gingivalis can invade periodontal ligament stem cells
title Porphyromonas gingivalis can invade periodontal ligament stem cells
title_full Porphyromonas gingivalis can invade periodontal ligament stem cells
title_fullStr Porphyromonas gingivalis can invade periodontal ligament stem cells
title_full_unstemmed Porphyromonas gingivalis can invade periodontal ligament stem cells
title_short Porphyromonas gingivalis can invade periodontal ligament stem cells
title_sort porphyromonas gingivalis can invade periodontal ligament stem cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5316216/
https://www.ncbi.nlm.nih.gov/pubmed/28212613
http://dx.doi.org/10.1186/s12866-017-0950-5
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